Caracterização macroscópica das gônadas durante a reprodução induzida em cativeiro do surubim-do-paraíba

Abstract

Resumo -O objetivo deste trabalho foi descrever a morfologia das gônadas do surubim-do-paraíba (F1) durante a reprodução induzida em cativeiro. Trinta fêmeas foram induzidas, tendo-se utilizado extrato hipofisário de carpa e gonadotrofina coriônica humana. Vinte e oito fêmeas responderam à indução, tendo liberado 238,80 g de ovócitos (taxa de fertilização: 24,33±8,35%). Após a extrusão dos gametas, os animais foram eutanasiados para análise morfológica das gônadas. Os testículos não apresentaram alterações morfológicas após a indução. Entre os ovários, 63,31% apresentaram má formação (desenvolvimento ímpar, não uniforme ou "afunilamento"), e o número de ovócitos liberados foi abaixo do obtido em reprodutores selvagens desta espécie.Termos para indexação: Steindachneridion parahybae, ovários, Rio Paraíba do Sul, testículos. Macroscopic characterization of gonads during induced breeding in captivity of surubim-do-paraíbaAbstract -The objective of this work was to describe the morphology of the gonads of surubim-do-paraíba (F1) during induced breeding in captivity. Thirty females were induced to reproduction using carp pituitary extract and human chorionic gonadotropin. Twenty-eight females responded to the induction, releasing 238.80 g of oocytes (fertilization rate: 24.33±8.35%). After stripping of gametes, the animals were euthanized for the morphological analysis of the gonads. Testicles did not present morphological alteration after induction. Among ovaries, 63.31% showed malformation (uneven, not uniform development or "narrowing"), and the number of released oocytes was below the obtained in wild broodstock of this species.

Mobilization of energetic substrates in the endangered catfish Steindachneridion parahybae (Siluriformes: Pimelodidae): changes in annual reproductive cycle in captivity

“…Then, they were randomly divided into two ponds (200 m 2 ) (duplicate) with concrete walls and a sandy bottom. During the experiment, the broodstocks were fed with commercial extruded feed for carnivorous fish containing 40% of crude protein at a rate of 2% biomass/day, offered twice a day (according to CESP fish farm routine), at 08:00 and 16:00h, following previously studies (Honji, 2011;Honji et al, 2012Honji et al, , 2013Honji et al, , 2016. During the experiment, the water temperature was 21.10 ± 0.14 ºC and the dissolved oxygen was 7.58 ± 0.36 mg/L, without significant variation between tanks.…”

“…It is a Neotropical catfish (gonochoristic, medium-sized), endemic to the Paraíba do Sul River Basin, critically endangered in the Brazilian red list, and considered regionally extinct in the state of São Paulo (Garavello, 2005;Caneppele et al, 2009;Honji et al, 2009Honji et al, , 2012Honji et al, , 2013Honji et al, , 2015Honji et al, , 2016Honji et al, , 2017. Besides this critical situation, the maintenance of S. parahybae female broodstock in fish farms is not entirely successful, as females do not reproduce when reared in captivity, i.e, females fail to ovulate or spawn naturally (Honji et al, 2015), despite their continuous feeding.…”

“…Since 2003, the energy company Companhia Energética de São Paulo (CESP) has funded studies on the biology of S. parahybae. These studies include the maintenance and management of broodstocks in captivity, egg and sperm quality, and successful larval rearing (Honji et al, 2009(Honji et al, , 2013(Honji et al, , 2015(Honji et al, , 2016(Honji et al, , 2017Sanches et al, 2013Sanches et al, , 2014Sanches et al, , 2015. They aim at ensuring successful and controlled breeding programs, involving conservation actions and a fish restocking program.…”

“…In the last few years, our research group and collaborators have advanced the knowledge on fundamental aspects of the reproductive biology of S. parahybae in captivity (Caneppele et al, 2009Honji et al, 2012Honji et al, , 2013Honji et al, , 2015Honji et al, , 2016Honji et al, , 2017Sanches et al, 2013Sanches et al, , 2014Sanches et al, , 2015Lopes et al, 2015). Knowledge of mobilization and deposition of energetic substrates on different tissues linked to their function in the reproductive cycle is required to understand the energetic requirement of S. parahybae, support further studies on broodstock nutrition, and contribute to the conservation of the species.…”

A case of intersex occurrence in Steindachneridion parahybae (Steindachner, 1877) (Siluriformes: Pimelodidae) under captivity condition: a cytogenetic and morphological study

“…A priori the macroscopic identification of the gonads was classified by the naked eye, using the Honji et al (2013) macroscopic characterization of adult S. parahybae gonads. After this procedure, gonads and liver were removed and weighed on analytical balance, accurate to the nearest thousandth of a gram (g), for calculate the gonadosomatic and hepatosomatic index and then, the middle third of the gonads were fixed in Bouin's solution for 24 h to confirm the sex for histological analysis.…”