The study of the vertical stratification of the forest evidences the dominance of certain species in relation to the ecological behavior. Additionally, it can also support in the definition of climbing methods for harvesting fruits and seeds in the tree canopy. The present work aimed to analyze the vertical structure based on the individuals that compose the different botanical families in the Seed Collection Area (SCA) belong Parakanã Indigenous Land (PIL), Pará state. The height of 10,383 individuals present at ACS of T.I. Parakanã was determined by the visual estimation method. Subsequently, five height classes (superior class 1, superior 2, middle 1, middle 2 and inferior) were defined for the individuals' distribution. By the multivariate analysis the families were grouped by similarity equal to 70%. The results showed that the families Anacardiaceae, Fabaceae, Lauraceae, Lecythidaceae and Moraceae belong to the SCA canopy. The different botanical families formed six groups considering similarity equal to 70%. Group 1 and 2, with 29 families, constitute the superior stratum of the forest, however, also have individuals in the inferior classes; groups 3 and 4, with 14 families, constitute the intermediate canopy and sub-forest; group 5, equivalent to 7 families, is located 100% in the intermediate canopy, and, in relation to group 6, formed only by 2 families, the individuals are in the total sub-forest. The different strata observed in the SCA of PIL evidences the need to use different climbing methods to harvest fruits and seeds.