Caracterização de um bacteriófago ativo contra &gt;i&lt;Xanthomonas campestris&gt;/i&lt; (Pammel) Dowson e &gt;i&lt;Xanthomonas vesicatoria&gt;/i&lt; (Doidge) Dowson e seu emprego no controle desses patógenos

Filho, Armando Bergamin

doi:10.11606/t.11.2019.tde-20191220-105322

Cited by 2 publications

(3 citation statements)

References 12 publications

(13 reference statements)

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“…The isolation of bacteriophages was carried out for both ltrates, from tomato leaves and soil, using the double-layer method of Adams' culture medium [12]. For this, 100 µL aliquots of the ltrate were added to 250 µL of bacterial suspension of the same isolate used in the enrichment process (1x10 8 CFU mL − 1 ) and incubated for 2 h at 28ºC.…”

Section: Sample Collectionmentioning

confidence: 99%

“…From this dilution, another 100 µL were taken and transferred to a new microtube containing 900 µL of SM-buffer solution as well. The Adams method [12] was used to create ten serial dilutions (1:10) to obtain Petri dishes with lysis plaques. This process was repeated three more times to obtain pure phage cultures.…”

Section: Sample Collectionmentioning

confidence: 99%

“…Then, chloroform was added, followed by centrifugation and ltration, as previously described. To concentrate the phages for use in isolation, serial dilutions of the last ltrate obtained were carried out, and the process was repeated, as described above [12].…”

Section: Sample Collectionmentioning

confidence: 99%

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Isolation, characterization, and evaluation of putative new bacteriophages for controlling bacterial spot on tomato in Brazil

de Sousa,

Janssen,

Rosa

et al. 2023

Arch Virol

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Bacterial spot is a highly damaging tomato disease caused by several species of the genus Xanthomonas. Bacteriophages have been studied for their potential use as a biological control method for bacterial diseases. Parte superior do formulário In the present study, bacteriophages were obtained from soil and tomato leaves in commercial elds in Brazil with the aim of obtaining biological control agents against bacterial spot. Phage isolation was carried out through co-cultivation with isolates of Xanthomonas euvesicatoria pv. perforans (Xp), which was prevalent in the collected areas. In the host range evaluation, no phage was able to induce a lytic cycle in all bacterial isolates. Then, in the in vivo tests, when Xpisolates were sensitive to the used phages, we observed that the prior application of phage suspension led to a reduction in the severity of the disease. The disease control level provided by phage application was equal to or greater than that obtained by the use of copper hydroxide. Electron microscopy analysis showed that all phages had similar morphology, with head-tails structure, similar to those viruses of the order Caudovirales. The observed short, non-contractile tubular tails strongly indicated that phages belong to the family Autographiviridae. Phylogenetic analysis con rmed it, and revealed that the phages belong to the genus Pradovirus. The phages are closely related to each other and potentially belong a new species of the genus. These phages will be evaluated in future studies against other tomato Xanthomonads strains to assess their potential as biological control agents.

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Section: Sample Collectionmentioning

confidence: 99%

Section: Sample Collectionmentioning

confidence: 99%

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Isolation, characterization, and evaluation of putative new bacteriophages for controlling bacterial spot on tomato in Brazil

de Sousa,

Janssen,

Rosa

et al. 2023

Arch Virol

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show abstract

Isolation, characterization and evaluation of putative new bacteriophage species to control bacterial spot on tomato in Brazil

Sousa

Janssen

Rosa

et al. 2023

Preprint

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Bacterial spot is a highly damaging tomato disease caused by several species of the genus Xanthomonas. Bacteriophages have been studied for their potential use as a biological control method for bacterial diseases. Parte superior do formulário In the present study, bacteriophages were obtained from soil and tomato leaves in commercial fields in Brazil with the aim of obtaining biological control agents against bacterial spot. Phage isolation was carried out through co-cultivation with isolates of Xanthomonas euvesicatoria pv. perforans (Xp), which was prevalent in the collected areas. In the host range evaluation, no phage was able to induce a lytic cycle in all bacterial isolates. Then, in the in vivo tests, when Xpisolates were sensitive to the used phages, we observed that the prior application of phage suspension led to a reduction in the severity of the disease. The disease control level provided by phage application was equal to or greater than that obtained by the use of copper hydroxide. Electron microscopy analysis showed that all phages had similar morphology, with head-tails structure, similar to those viruses of the order Caudovirales. The observed short, non-contractile tubular tails strongly indicated that phages belong to the family Autographiviridae. Phylogenetic analysis confirmed it, and revealed that the phages belong to the genus Pradovirus. The phages are closely related to each other and potentially belong a new species of the genus. These phages will be evaluated in future studies against other tomato Xanthomonads strains to assess their potential as biological control agents.

show abstract

Caracterização de um bacteriófago ativo contra >i<Xanthomonas campestris>/i< (Pammel) Dowson e >i<Xanthomonas vesicatoria>/i< (Doidge) Dowson e seu emprego no controle desses patógenos

Cited by 2 publications

References 12 publications

Isolation, characterization, and evaluation of putative new bacteriophages for controlling bacterial spot on tomato in Brazil

Isolation, characterization, and evaluation of putative new bacteriophages for controlling bacterial spot on tomato in Brazil

Isolation, characterization and evaluation of putative new bacteriophage species to control bacterial spot on tomato in Brazil

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