Abstract:O presente trabalho caracteriza a região 3'-terminal do genoma de um isolado do Southern bean mosaic virus encontrado no Estado de São Paulo (SBMV-SP). O RNA foi extraído de partículas virais purificadas e submetido a RT-PCR usando oligonucleotídeos desenhados para amplificar 972 nt da região 3'-terminal do RNA viral. Foi obtido fragmento de tamanho esperado que inclui o gene da proteína capsidial e a região 3'-terminal não codificadora. O gene da proteína capsidial (ORF4) contém 801 nucleotídeos, incluindo-se… Show more
“…The sequence that starts at position 2173 is GGGGTCC, not GGGAGTCC, and the one starting at position 2217 is GAA instead of TAA. Together, the ORF2 sequence reported here and those previously described from the 5′ end (Espinha et al., 2004) and the 3′ end (Espinha et al., 2005) show that the SBMV SP genome is 4132 nt long (GenBank ). The 5′ end starts with an untranslated region with 92 nt and the first open reading frame (ORF1), starting at the first AUG at position 93 and ending at a UGA stop codon at position 536.…”
Section: Resultssupporting
confidence: 74%
“…In Brazil, SBMV was first detected in bean plants in the Federal District region (Cupertino et al., 1982), and later in the States of São Paulo (Costa, 1987) and Paraná (Gasparin et al., 2005). Some molecular properties of the São Paulo isolate (SBMV SP ) were determined (Moreira and Gaspar, 2002), and the 5′ and 3′ termini were sequenced (Espinha et al., 2004, 2005). The SBMV SP coat protein sequence showed identity of nucleotides and amino acids of 91 and 93%, respectively, with the Arkansas isolate (SBMV ARK ) described in the USA (Lee and Anderson, 1998).…”
The sequence of the central part (ORF2) of a Brazilian isolate of Southern bean mosaic virus (SBMV SP ) is described. This ORF is 2888 nt long and together with the previously-sequenced 5¢ and 3¢ ends provides the complete nucleotide sequence of this virus isolate. The SBMV SP RNA encodes four overlapping open reading frames (ORF1, ORF2a, ORF2b, ORF4) and has a genome organization similar to that of the Cocksfoot mottle sobemovirus.
“…The sequence that starts at position 2173 is GGGGTCC, not GGGAGTCC, and the one starting at position 2217 is GAA instead of TAA. Together, the ORF2 sequence reported here and those previously described from the 5′ end (Espinha et al., 2004) and the 3′ end (Espinha et al., 2005) show that the SBMV SP genome is 4132 nt long (GenBank ). The 5′ end starts with an untranslated region with 92 nt and the first open reading frame (ORF1), starting at the first AUG at position 93 and ending at a UGA stop codon at position 536.…”
Section: Resultssupporting
confidence: 74%
“…In Brazil, SBMV was first detected in bean plants in the Federal District region (Cupertino et al., 1982), and later in the States of São Paulo (Costa, 1987) and Paraná (Gasparin et al., 2005). Some molecular properties of the São Paulo isolate (SBMV SP ) were determined (Moreira and Gaspar, 2002), and the 5′ and 3′ termini were sequenced (Espinha et al., 2004, 2005). The SBMV SP coat protein sequence showed identity of nucleotides and amino acids of 91 and 93%, respectively, with the Arkansas isolate (SBMV ARK ) described in the USA (Lee and Anderson, 1998).…”
The sequence of the central part (ORF2) of a Brazilian isolate of Southern bean mosaic virus (SBMV SP ) is described. This ORF is 2888 nt long and together with the previously-sequenced 5¢ and 3¢ ends provides the complete nucleotide sequence of this virus isolate. The SBMV SP RNA encodes four overlapping open reading frames (ORF1, ORF2a, ORF2b, ORF4) and has a genome organization similar to that of the Cocksfoot mottle sobemovirus.
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