Caracterização clínica e histopatológica e tipagem do papilomavírus humano das verrugas vulgares nos receptores de transplante renal

Abstract

Abstract:The prevalence of skin lesions caused by the human papillomavirus (HPV) is high in kidney transplant patients. Twenty recipients of kidney transplants with a diagnosis of common warts were evaluated. HPV detection was performed by polymerase chain reaction (PCR) using the MY09/MY11 and RK91 primers. HPV typing was performed by restriction fragment length polymorphism analysis and direct sequencing. The presence of HPV was identified in 10 patients (50%) and the types identified were HPV-2, 27, 29, 34 and 57. ©2010 by Anais Brasileiros de DermatologiaKidney transplant recipients have a 15-50% likelihood of developing common warts associated with the human papillomavirus (HPV) in the first year following the transplant and a 77-95% likelihood within five years. 1 With respect to the detection and typing of HPV in common warts, few publications are available of studies conducted in the Brazilian population, the only study having been carried out in an immunocompetent population infected by the human immunodeficiency virus (HIV) and in patients with epidermodysplasia verruciformis. 3 Therefore, the objective of the present study was to evaluate the clinical and histopathological characteristics of common warts in kidney transplant recipients and identify the types of HPV present in the lesions.Twenty kidney transplant recipients with a clinical and histopathological diagnosis of common warts were evaluated and submitted to HPV detection (using polymerase chain reaction [PCR] with the MY09/11 and RK91 primers) and typing (using restriction fragment length polymorphism analysis [RFLP] and/or direct sequencing).The mean age of the patients was 47.9 years. There was no difference in the incidence of the condi-

Hyperproliferative action of bovine papillomavirus: genetic and histopathological aspects

“…It has also been described in canine (Fernandes et al, 2009), rabbit (Rashad andEvans, 1967) and human papillomas (Martelli-Marzagão et al, 2010). Keratohyalin granules are rich in sulfur amino acids, which are associated with the keratinization process, resulting in hyperkeratosis, which was observed in all analyzed papilloma samples.…”

“…Parakeratotic hyperkeratosis is indicative of tissue hyper-differentiation, and is a common finding associated with papillomavirus infection (Martelli-Marzagão et al, 2010). Hyperkeratosis is a result of mitogenic stimulation induced by BPV oncoproteins, which is associated with the absence of phosphatases and oxidases.…”

Efficacy and safety of local candida immunotherapy in recalcitrant warts in pediatric kidney transplantation: A case report

“…The most common immunosuppressive regimen in these patients was tacrolimus and prednisone, in combination with either azathioprine or mycophenolate mofetil (MMF). The most commonly seen human papillomavirus (HPV) strains responsible for common warts are HPV-2, 27, 29, 34 and 57[ 5 ]. Painful warts can impair the quality of life and cause significant morbidity[ 6 ].…”

Isolamento e identificação do papilomavírus bovino em grupo experimental de bovinos para obtenção de um banco de vírus.

“…A coilocitose resulta da cooperação entre as oncoproteínas E5 e E6, sendo frequentemente observada nas camadas mais diferenciadas, onde é encontrada uma grande quantidade de partículas virais formadas (Krawczy et al, 2008). Embora as vias envolvidas na vacuolização perinuclear sejam desconhecidas, acredita-se que ela possa contribuir para a fragilidade dos queratinócitos, facilitando a liberação de partículas virais para o meio ambiente (Krawczy et al, 2008 (Cheville, Olson, 1964;Sousa et al, 2011), sendo a principal hiperqueratose observada em infecções decorrentes de PVs (Fernandes et al, 2009;Martelli-Marzagão et al, 2010;Velazquez et al, 2012 Matoltsy, 1970). A hipergranulose se caracteriza por uma abundante quantidade de grânulos de cerato-hialina na camada granulosa, onde a presença de vírions já foi observada através de microscopia eletrônica de transmissão (Rashad, Evans, 1967), tendo sido observada, também, em papilomas de cães (Fernades et al, 2009), coelhos (Rashad, Evans, 1967) e humanos (Martelli-Marzagão et al, 2010).…”