Abstract:LesA, from the citrus canker pathogen Xanthomonas axonopodis pv. citri (Xac), is an α/β hydrolase fold protein with lipase/esterase function encoded by the gene XAC0501. In silico analysis revealed that LesA gene encodes a lipase/esterase orthologous to the LipA enzyme, which is present in several species of Xanthomonas. In this study, we aimed to biochemically characterize LesA, and to determine the importance of this protein in the virulence of Xac. A LesA mutant was generated, and lipase and esterase activi… Show more
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