Caracterização biológica do muco epidérmico da arraia de água doce >i<Paratrygon aiereba>/i<

Abstract

TURÍBIO, Thompson de Oliveira. Biological characterization of the epidermic mucus of freshwater stringray Paratrygon aiereba. 2018. 85p. Tese (Doutorado em tecnologia nuclear) -Instituto de Pesquisas Energéticas e Nucleares -IPEN-CNEN/SP. São PauloThe epidemiology of the diseases by venomous fish is little reported, despite high morbidity and relevance to health. In the central region of Brazil, the Tocantins and Araguaia River Basins are widely used for recreational activities, especially between June and August. Despite the difficulty of identifying the animal causing the accident by the injured, most of the works affirms that the genus Potamotrygon is the most related to these diseases. On the other hand, stingrays of the genus Paratrygon, although presenting a stinger smaller than stingrays of other genera, its causes enough accidents by camouflaging themselves easily due to their light color, confusing with the ground of the rivers and lakes. The objective was to identify biological activities of the Paratrygon aiereba stingray´s mucus. The effects of the edemaciant activity of stingray´s mucus were evaluated, through a sample design composed of groups of six female Swiss mice (25-30 g) were inoculated with 50 μL in the hind paws, receiving 25, 50 and 100 μg of mucus in the right paw, and in the left paw PBS solution (negative control). The dynamics of the alteration in the microcirculatory system was determined using intravital microscopy, by transillumination of the cremaster muscle after topical application of the Paratrygon aiereba mucus. Administration of 20 μl of sterile PBS was used as a control. Groups of 6 male Swiss mice (25-30 g) were inoculated with 30 μL containing the amounts of 12.5 μg, 25 μg, 50 μg, 100 μg and 200 μg of mucus. Female Swiss mice (25-30 g) (n = 30) were placed individually under inverted glass funnels located on a reflecting surface to facilitate observation. To verify cell damage, the animals chosen for the experiment were the female Swiss mice (n = 30 animals). These were divided into 6 treatments each one with five animals, one control group and five test groups (12.5 μg / 50 μl, 25 μg / 50 μl, 50 μg / 50 μl, 100 μg / 50 μl and 200 μg / 50 μl). In relation to poisoning by stingray accident, the effects of mucus on necrotizing activity were studied. Groups of 6 female Swiss mice (25-30 g) were inoculated with 50 μL of the mucus samples (200 μg) and PBS (negative control) on the dorsum. Analyzing the behavior of the edemaciant activity in relation to the time, it is noticed that the effect of the edema is more accentuated in the first hours of the accident; however, it is found that after 4h of the accident this action tends to decrease. With regard to nociceptive activity, it can be observed that the test concentrations showed a significant difference in relation to the PBS control (p <0.05). At the end of the 30 minutes of the experiment, it was noted that there was a concentration of 25 μg of mucus, approximately 4-fold increase over baseline time. Cellular lesion levels wi...