Caractéristiques biochimiques des lithiases vésiculaires par spectrométrie infrarouge à Abidjan

P, Djessou; Monde, Alexandra; Tiahou, G; Koffi, Gnagne-Agnero; Camara, Moussa Kantara; Djohan, F; Yapo, E; Kassayou, S; Peuchant, E.; Sess, D.; Monnet, D.

doi:10.1684/abc.2010.0400

Cited by 3 publications

(2 citation statements)

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“…Control spectrums were acquired from standard substances, which were of high quality (99% pure) and purchased from Sigma Chemical Company (St. Louis, MO, USA). Through comparisons of the spectra between gallbladder stones and standard controls, gallbladder stones were grouped as cholesterol stones, pigment stones or mixed stones [25], [26].…”

Section: Methodsmentioning

confidence: 99%

Cholecystolithiasis Is Associated with Clonorchis sinensis Infection

Qiao

Luo

et al. 2012

PLoS ONE

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BackgroundThe objective of this study was to analyze gallbladder stones for direct evidence of a relationship between Clonorchis sinensis infection and gallbladder stones formation.MethodologyWe investigated one hundred eighty-three gallbladder stones for the presence of Clonorchis sinensis eggs using microscopy, and analyzed their composition using Fourier transform infrared spectroscopy. We confirmed the presence of Clonorchis sinensis eggs in the gallbladder stones using real-time fluorescent PCR and scanning electron microscopy.Principal Findings Clonorchis sinensis eggs were detected in 122 of 183 gallbladder stones based on morphologic characteristics and results from real-time fluorescent PCR. The proportion of pigment stones, cholesterol stones and mixed gallstones in the egg-positive stones was 79.5% (97/122), 3.3% (4/122) and 17.2% (21/122), respectively, while 29.5% (18/61), 31.1% (19/61) and 39.3% (24/61) in the egg-negative stones. The proportion of pigment stone in the Clonorchis sinensis egg-positive stones was higher than in egg-negative stones (P<0.0001). In the 30 egg-positive stones examined by scanning electron microscopy, dozens or even hundreds of Clonorchis sinensis eggs were visible (×400) showing a distinct morphology. Many eggs were wrapped with surrounding particles, and in some, muskmelon wrinkles was seen on the surface of the eggs. Also visible were pieces of texture shed from some of the eggs. Some eggs were depressed or without operculum while most eggs were adhered to or wrapped with amorphous particles or mucoid matter (×3000).Conclusion Clonorchis sinensis eggs were detected in the gallbladder stones which suggests an association between Clonorchis sinensis infection and gallbladder stones formation, especially pigment stones.

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Section: Methodsmentioning

confidence: 99%

Cholecystolithiasis Is Associated with Clonorchis sinensis Infection

Qiao

Luo

et al. 2012

PLoS ONE

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“…Gallbladder stones can be divided into four groups according to their location: intracavitary, cystic duct, intramural, and mucosal stones (small stones adhering to the mucosa) [5]. Based on their main component, gallbladder stones are divided into cholesterol stones, bile pigment stones, mixed stones, and other types [6-9] (including calcium carbonate stones, calcium phosphate stones, fatty acid stones, and cystine stones); this classification is mainly based on the infrared spectrum of the stones. Compared with other types of stones, cystine stones are rare in the gallbladder, although they are common in the urinary system.…”

Section: Introductionmentioning

confidence: 99%