Características mamográficas do câncer de mama associadas aos polimorfismos GSTM1 e GSTT1

Morais, Lívia Martins Tavares Scianni; Filho, Cássio Cardoso; Lourenço, Gustavo Jacob; Shinzato, Júlia Yoriko; Zeferino, Luíz Carlos; Lima, Carmen Sílvia Passos; Gurgel, Maria Salete Costa

doi:10.1590/s0104-42302008000100022

Cited by 10 publications

(8 citation statements)

References 18 publications

(47 reference statements)

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“…Hatagima et al (2000) observed frequencies of 48.6 and 46.4%, respectively, for the GSTM1-null genotype in a population from Brasilia and Rio de Janeiro. Morais et al (2008) found that 44.4% of the individuals analyzed in Campinas (São Paulo) carried the GSTM1-null genotype. In a study carried out in Bahia, they found that the GSTM1-null polymorphism was less prevalent (43.9%) in that population.…”

Section: Discussionmentioning

confidence: 99%

“…Epidemiological studies have suggest that individuals with homozygous deletion of GSTM1 and GSTT1 have a higher risk of developing several types of neoplasias (Morais et al, 2008). Variability in the phenotype distribution of null GSTM1 and GSTT1 genotypes has been reported in different populations, particularly in well-defined ethnic groups (Gattás et al, 2004).…”

Section: Introductionmentioning

confidence: 99%

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Polymorphisms of GSTM1, GSTT1, and p53 in Goiânia, Goiás

Rodrigues¹,

Costa²,

Martins³

et al. 2015

Genet. Mol. Res.

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ABSTRACT. Genetic polymorphisms are defined as changes within the DNA sequences of genes that have frequencies in the population higher than 1%. The glutathione S-transferases play an important role in the cellular detoxification systems involved in oxidative stress that can lead to accumulation of reactive oxygen species. Epidemiological studies have suggested that individuals with homozygous deletion of glutathione S-transferase mu 1 (GSTM1) and glutathione S-transferase theta 1 (GSTT1) are at higher risk of developing several types of neoplasias. The p53 protein is highly expressed in tumors and transformed cells, and the p53 is a classical tumor suppressor gene involved in regulating cell growth and development. In this study, we investigated the prevalence of polymorphisms in the p53, GSTM1, and GSTT1 genes in a population from Goiânia. We evaluated the polymorphisms of these genes in peripheral blood samples. The null or present polymorphism of GSTM1 and GSTT1 17359Polymorphisms of GSTM1, GSTT1, and p53 in Goiânia, Goiás ©FUNPEC-RP www.funpecrp.com.br Genetics and Molecular Research 14 (4): 17358-17365 (2015) genes and Arg/Pro of the p53 gene were analyzed. Our results revealed a higher frequency of the GSTM1-null polymorphism (72.4%) than the GSTM1-present genotype (27.6%). For GSTT1, we observed higher frequency for the null genotype (65.5%) compared to the present genotype (34.5%). Analysis of p53 gene polymorphisms showed a higher frequency for the genotype Arg/Pro (66%) and a lower frequency for the Arg/Arg (23%) and Pro/Pro (11%) genotypes. It is essential to understand polymorphism frequencies in different populations and to evaluate the role of genetic polymorphisms and their effects on health.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Polymorphisms of GSTM1, GSTT1, and p53 in Goiânia, Goiás

Rodrigues¹,

Costa²,

Martins³

et al. 2015

Genet. Mol. Res.

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“…GSTs are a family of intracellular enzymes of detoxification phase II that catalyze diverse electrophilic compounds conjunction to GSH, blocking DNA mutations of the cells [9]. …”

Section: Introductionmentioning

confidence: 99%

Expression of glutathione, glutathione peroxidase and glutathione S-transferase pi in canine mammary tumors

et al. 2014

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BackgroundGlutathione (GSH) is one of the most important agents of the antioxidant defense system of the cell because, in conjunction with the enzymes glutathione peroxidase (GSH-Px) and glutathione S transferase pi (GSTpi), it plays a central role in the detoxification and biotransformation of chemotherapeutic drugs. This study evaluated the expression of GSH and the GSH-Px and GSTpi enzymes by immunohistochemistry in 30 canine mammary tumors, relating the clinicopathological parameters, clinical outcome and survival of the bitches. In an in vitro study, the expression of the genes glutamate cysteine ligase (GCLC) and glutathione synthetase (GSS) that synthesize GSH and GSH-Px gene were verified by qPCR and subjected to treatment with doxorubicin, to check the resistance of cancer cells to chemotherapy.ResultsThe immunohistochemical expression of GSH, GSH-Px and GSTpi was compared with the clinical and pathological characteristics and the clinical outcome in the bitches, including metastasis and death.The results showed that high immunoexpression of GSH was correlated to the absence of tumor ulceration and was present in dogs without metastasis (P < 0.05). There was significant correlation of survival with the increase of GSH (P < 0.05). The expression of the GSH-Px and GSTpi enzymes showed no statistically significant correlation with the analyzed variables (p > 0.05). The analysis of the relative expression of genes responsible for the synthesis of GSH (GCLC and GSS) and GSH-Px by quantitative PCR was done with cultured cells of 10 tumor fragments from dogs with mammary tumors.The culture cells showed a decrease in GCLC and GSS expression when compared with no treated cells (P < 0.05). High GSH immunoexpression was associated with better clinical outcomes.ConclusionTherefore, high expression of the GSH seems to play an important role in the clinical outcome of patients with mammary tumors and suggest its use as prognostic marker. The in vitro doxorubicin treatment significantly reduces the expression of GCLC and GSS genes so we can consider them to be candidates for predictive markers of therapeutic response in mammary cancer.

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“…A GST é uma família de enzimas intracelulares localizadas no citosol que modulam a ação de toxinas endógenas e exógenas sobre as células, evitando danos ao DNA (MANNERVIK et al, 1985;MORAIS et al, 2008; CASTRO e LIMA, 2013). Este grupo de enzimas tem sido amplamente estudado, pois estão envolvidas no metabolismo de muitos carcinógenos, poluentes ambientais e drogas anticancerígenas.…”

Section: Polimorfismo Gstm1 No Gene Da Gstunclassified

“…A GST faz parte de uma família de enzimas intracelulares que modullam a ação de toxinas endógenas e exógenas sobre as células, evitando danos ao DNA (MANNERVICK, 1985). Esta família de enzimas está envolvida no metabolismo de muitos carcinógenos, poluentes ambientais e drogas anticancerígenas, por isso a inexistência de isoenzimas específicas pode gerar efeito significativo na tolerância do organismo ao estresse oxidativo (ZHENG et al, 2002;MORAIS et al, 2008). O gene da classe mu (GSTM1), abordado nesta pesquisa, é polimórfico em humanos e estão ausentes ou deletados de forma homozigótica em 40 a 50% em diferentes populações étnicas (NELSON et al 1995;HATAGIMA et al, 2000;GISNBERG et al, 2009).…”

Section: Polimorfismo Gstm1unclassified

Status de selênio de uma população residente em área de risco de contaminação por mercúrio. Influência de polimorfismos e ação sobre o estresse oxidativo

Rocha¹

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por serem apoio, companhia e ajuda nos trabalhos realizados no cotidiano. Especialmente, À Bruna Reis (Rabin) e Isabela Saraiva (Isa nojentinha do meu coração), que me proporcionaram uma amizade de cunho profissional e pessoal, tornando esta jornada mais viva, leve e muito alegre! Por todos os braços e abraços servidos nos momentos de ajuda sem horário para acabar. Por me fazerem lembrar desse trio com um enorme sorriso no rosto! À Kaluce Almondes (Kaká), querida companheira de análises que pareciam intermináveis (risos), obrigada pela parceria, conversas filosóficas e gargalhadas constantes, inclusive em momentos de aflição. À Liliane Viana Pires (Lili medalhinha), luz em todos os momentos (desde 2006). Mesmo com a distância geográfica existente entre nós, você é sempre presente! Agradeço ainda, por despertar em mim a vontade de ser sempre melhor. Às florzinhas da Liliane Pires, Leila Hashimoto (Leilinha), Luciane Alencar (Lu) e Verônica Bandeira (Vê), pela ajuda durante as análises de enzimas, mesmo com tantos afazeres e por me tratarem sempre com muito carinho e atenção. À Bárbara Cardoso, pelo abraço apertado que diz muito em cada encontro! Às Fernandas, Santana e Shinagawa, agradeço a ajuda, a companhia, a diversão e a amizade construída, principalmente, nos momentos extras de trabalho, já que nem sempre conseguíamos acabar no horário do expediente em virtude do número de amostras (risos). À Lilian Mendes, apoio e alicerce fundamentais nos últimos meses, que através do seu coração, profissionalismo e vivência de mundo, ensinou-me a aceitar e conviver com o meu eu interior e com a minha alma! Ao meu irmão de coração, Guilber Diniz Barros, pela força e energia enviada, por todas as conversas na tentativa de me distrair ou esquecer algo, por me ajudar à distância, e principalmente, por me ensinar a levantar sem lágrimas nos olhos e de cabeça erguida. À Fabiana Yasuhara, da Assessoria Científica Life Technologies, pelo suporte fundamental realizado durante as análises do polimorfismo de deleção GSTM1. À funcionária Maria de Lurdes Pedrosa (Lurdinha), pelo otimismo, carinho e pelas palavras "no fim tudo dá certo".

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Características mamográficas do câncer de mama associadas aos polimorfismos GSTM1 e GSTT1

Cited by 10 publications

References 18 publications

Polymorphisms of GSTM1, GSTT1, and p53 in Goiânia, Goiás

Polymorphisms of GSTM1, GSTT1, and p53 in Goiânia, Goiás

Expression of glutathione, glutathione peroxidase and glutathione S-transferase pi in canine mammary tumors

Status de selênio de uma população residente em área de risco de contaminação por mercúrio. Influência de polimorfismos e ação sobre o estresse oxidativo

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