Caracterisation of Puccinia hemerocallidis causing the first outbreak of daylily rust in Europe

Diploid Nuclei Occur throughout the Life Cycles of Pucciniales Fungi

“…); Rs — Raphanus sativus , 1.11 pg DNA/2C ( 28 ) (used for Coleosporium tussilaginis f. sp. tussilaginis , Puccinia difformis , P. obscura and P. poarum ); Ra — Rhamnus alaternus , 0.680 pg DNA/2C ( 29 ) (used for Puccinia galactitis , P. mesnieriana , P. phragmitis and P. verruca ); Sl — Solanum lycopersicum , 1.96 pg DNA/2C ( 28 ) (used for Gymnosporangium sp., Miyagia pseudosphaeria and Puccinia smyrnii ); Vf — Vicia faba , 26.65 pg DNA/2C ( 28 ) (used for Hemerocallis sp.). d Unpublished values available at the Plant DNA C-values Database, Royal Botanic Gardens, Kew and attributed to: Band, S. R. (personal.…”

“…Fungal ploidy level and nuclear DNA content were determined by flow cytometry using the protocols by Tavares et al ( 5 ) for Pucciniales fungi, by Sabatinos and Forsburg ( 25 ) for yeasts and by Bourne et al ( 26 ) adapted by Pires et al ( 27 ) for cultivable filamentous fungi. For DNA content estimation, plant and fungal standards used were, respectively, S olanum lycopersicum ( 28 ), Raphanus sativus ( 28 ) and Rhamnus alaternus ( 29 ), and Inonotus hispidus , Colletotrichum acutatum and Cenococcum geophilum ( 30 ) ( Table 1 ). Analyses were conducted in a CyFlow Space flow cytometer (Sysmex, Norderstedt, Germany) equipped with a 30 mW green solid-state laser emitting at 532 nm for optimal propidium iodide excitation and emission at 617 nm (532/617 nm), as previously described ( 31 ).…”