Molecular characterization and genetic relationships among most common identified morphotypes of critically endangered rare Moroccan species Argania spinosa (Sapotaceae) using RAPD and SSR markers

Abstract

. Molecular characterization and genetic relationships among most common identified morphotypes of critically endangered rare Moroccan species Argania spinosa (Sapotaceae) using RAPD and SSR markers. Annals of Forest Science, Springer Verlag/EDP Sciences, 2008, 65 (8), pp.1. <hal-00883463>Ann. For. Sci. 65 (2008) Abstract• The objective of this work is the molecular characterization of most common identified morphotypes of critically endangered rare Moroccan species Argania spinosa.• Eighteen RAPD markers and twenty SSR markers have been assayed in 38 argan tree accessions from the three most commonly identified morphotypes: oval, spherical and spindle fruit types.• A total of 140 polymorphic RAPD bands were detected out of 146 bands. The number of presumed alleles revealed by the SSR analysis ranged from 1 to 5 alleles per locus with a total number of 32 alleles identified. Results demonstrated an extensive genetic variability within the tested argan accessions. RAPDs presented a high level of polymorphism and greater information content than SSRs.• Our results could indicate that Vitellaria paradoxa is genetically closer to argan than Manilkara huberi. In addition, the correlation between the clustering based on RAPD and SSR markers were in general low. The observed clustering could be better explained according to geographic proximity than morphotype. For this reason the traditional morphological characterization of the argan accessions in morphotypes (according to fruit phenotype) seem not to be in correlation to the real genetic background (genotype) of this specie. The implications of these results in the creation of effective germplasm core collection in argan have been also discussed. Mots-clés :arganier / Argania / collection noyau / relations genetiques / RAPD / Sapotaceae / SSR / taxonomie / transférabilité Résumé -Caractérisation moléculaire et relation génétique entre les morphotypes les plus connus d'une espèce marocaine rare et menacée, Argania spinosa (Sapotaceae) à l'aide de marqueurs RAPD et SSR.• L'objectif de ce travail consiste en la caractérisation moléculaire et l'étude des relations génétiques entre des morphotypes identifiés chez une espèce marocaine rare et menacée, l'arganier (Argania spinosa).• Pour cela, dix-neuf marqueurs RAPD et vingt marqueurs SSR on été testés dans 38 accessions d'arganier des trois morphotypes du fruit identifiés : ovale, sphérique et broche.• En utilisant la technique d'amplification au hasard de l'ADN (RAPD), 140 fragments polymorphes ont été détectés parmi les 146 amplifiés. Le nombre d'allèle révélé par l'analyse des marqueurs microsatelittes (SSRs) varie de un à cinq allèles par locus avec un total de 32 allèles identifiés. Les résultats démontrent une grande variabilité génétique au sein des accessions d'arganier testées. Les marqueurs RAPD présentent un taux de polymorphisme et une hétérozygotie attendue supérieurs aux SSR.• Les résultats indiquent également que Vitellaria paradoxa est plus proche génétiquement de l'arganier que Manilkara huberi. La co...

Assessment of Genetic Diversity of Argania spinosa L. Growing in Arid and Semi-arid Areas of Morocco as Revealed by Inter-Simple Sequence Repeats

“…The authors' results showed that the genetic diversity of argan tree is relatively higher (PB% = 98.8% and H = 0.172) contrary to what has been reported by other studies on the same species (allozymes analysis [18] and RAPD [15,19]…”

“…Although the genetic diversity of argan tree has been studied using agro-morphological [13][14][15][16] and biochemical markers [17,18], only few studies used molecular markers. Thus, Bani-Aameur and Benlahbil [15] used random amplified polymorphic DNA (RAPD) markers, Majourhat et al [19] utilized simple sequence repeats (SSR) markers to characterize the most common morphotypes and El-Mousadik and Petit [17] identified the haplotypes of this endangered species using PCR-restriction fragment length polymorphism (RFLP) of chloroplast DNA.…”

Study of genetic diversity and differentiation of argan tree population (Argania spinosa L.) using AFLP markers

“…To date, most of A. spinosa L. studies have been mainly focused on its fruit and oil (Alaoui et al, 1998;Bani-Aameur et al, 1999;Charrouf and Guillaume, 1999;Charrouf et al, 2002;Sparg et al, 2004;Harhar et al, 2014). However, there are few investigations that show basic information regarding the genetic variation within/among populations A. spinosa L. (El Mousadik and Petit, 1996;Majourhat et al, 2008), and AFLP markers have, to the best of our knowledge, never been used to analyze the genetic diversity of A. spinosa L.…”

Methodology Analysis of genetic diversity in Larix gmelinii (Pinaceae) with RAPD and ISSR markers

“…This slight variation may be caused by primer detection mechanisms: 1) the loci detected by RAPD and ISSR were different; for RAPD, the inverted repeats and internal site were amplified, whereas ISSR amplified inter-segment repeats, and 2) RAPD primers used random sequences of 10 bases, and ISSR primers were based on anchored primer repeats of 15-24 bases; therefore, polymorphism would be different, and the number of polymorphic fragments would increase with the increased number of primers and could also cause the change in genetic distance. Classification results were different by using different technology, as has been published in several studies (Messaoud et al, 2007;Majourhat et al, 2008).…”