Caracterisation isotypique des anticorps specifiques dans la distomatose humaine a Fasciola hepatica

Pailler, S.; Puygauthier-Toubas, D.; Bonnin, A.; Marx-Chemla, C.; Camerlynck, P.; Thoannes, H.; Pinon, J. M.

doi:10.1016/s0399-077x(05)80188-x

Cited by 5 publications

(1 citation statement)

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“…They include the enzyme-linked immunofiltration assay (ELIFA) (Pailler et al 1990), the enzyme-linked immuno-electrotransfer blot (EITB) or western blot (Santiago and Hillyer, 1986; Hillyer and Soler De Galanes, 1988; Hillyer et al 1992; Silva et al 1993; Shaker et al 1994; Hammami et al 1997; Marcos et al 2008; Trifina et al 2011; Agnamey et al 2012) and the Falcon™ assay screening test-enzyme linked immunosorbent assay (FAST-ELISA) (Hillyer and Soler De Galanes, 1988, 1991; Hillyer et al 1992; Bjorland et al 1995; Rahimi et al 2011; Elsibaei et al 2013).…”

Section: Diagnosis With Blood Samplesmentioning

confidence: 99%

Diagnosis of human fascioliasis by stool and blood techniques: update for the present global scenario

2014

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Before the 1990s, human fascioliasis diagnosis focused on individual patients in hospitals or health centres. Case reports were mainly from developed countries and usually concerned isolated human infection in animal endemic areas. From the mid-1990s onwards, due to the progressive description of human endemic areas and human infection reports in developing countries, but also new knowledge on clinical manifestations and pathology, new situations, hitherto neglected, entered in the global scenario. Human fascioliasis has proved to be pronouncedly more heterogeneous than previously thought, including different transmission patterns and epidemiological situations. Stool and blood techniques, the main tools for diagnosis in humans, have been improved for both patient and survey diagnosis. Present availabilities for human diagnosis are reviewed focusing on advantages and weaknesses, sample management, egg differentiation, qualitative and quantitative diagnosis, antibody and antigen detection, post-treatment monitoring and post-control surveillance. Main conclusions refer to the pronounced difficulties of diagnosing fascioliasis in humans given the different infection phases and parasite migration capacities, clinical heterogeneity, immunological complexity, different epidemiological situations and transmission patterns, the lack of a diagnostic technique covering all needs and situations, and the advisability for a combined use of different techniques, at least including a stool technique and a blood technique.

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