Caractérisation enzymatique deTrichogramma evanescens et deT. Maidis [Hym.: Trichogrammatidae]; Étude des hybrides

Pintureau, Bernard; Babault, Manuel

doi:10.1007/bf02371830

Cited by 36 publications

(14 citation statements)

References 9 publications

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“…Thelytoky in T. cacoeciae could have originated from interspecific hybridisation (Pinto and Stouthamer, 1994), which has been described for T. vogelei Â T. brassicae (Pintureau and Babault, 1981) and T. exiguum Â T. californicum (Nagarkatti, 1970). Hybridisation-induced parthenogenesis is likely caused by epistatic interactions between parental genomes.…”

Section: Discussionmentioning

confidence: 99%

Cytogenetic mechanism and genetic consequences of thelytoky in the wasp Trichogramma cacoeciae

2004

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In Hymenoptera, complete parthenogenesis, that is thelytoky, is a common phenomenon where virgin females produce only daughters. Thelytoky is often induced by bacteria of the genus Wolbachia, but can also be genetically determined by the insect itself, as in the genus Trichogramma where both forms exist. In order to compare these two forms of thelytoky, chromosome behaviour analysis in young eggs and genetic analysis of microsatellite markers were carried out in the wasp Trichogramma cacoeciae, where thelytoky is genetically determined. Microscopic studies revealed that during female gamete formation meiotic cells undergo only a single equational division followed by the expulsion of a single polar body. This absence of meiotic recombination and reduction corresponds well with the high levels of heterozygosity observed in females collected from the field and a nonsegregation pattern in the offspring of heterozygous females. We therefore concluded that diploidy in T. cacoeciae is maintained through an apomictic cloning mechanism and that the incidence of thelytoky under genetic control of the wasp differs entirely from the mechanism induced by Wolbachia infection, where thelytoky is restored through gamete duplication.

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Section: Discussionmentioning

confidence: 99%

Cytogenetic mechanism and genetic consequences of thelytoky in the wasp Trichogramma cacoeciae

2004

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“…That offspring from inter-specific crosses which display morphological characteristics specific to both parents should possess DNA from each is not surprising. Hybridization has been reported in the gregarious Trichogramma (Nagarkatti and Fazaluddin, 1973;Pintureau and Babault, 1981), although these studies were similarly limited to the laboratory and parental species identity concerns were raised. Genetic studies have revealed hybridization is more common than first thought in the social Hymenoptera, such as ants, where sperm parasitism and genetic caste determination have been reported (see Nonacs, 2006), and honeybees, where africanization and introgression of feral European honeybee colonies is expanding from the American tropics into the United States (Pinto et al, 2005).…”

Section: Hybridizationmentioning

confidence: 99%

Parental genetic traits in offspring from inter-specific crosses between introduced and indigenous Diadegma Foerster (Hymenoptera: Ichneumonidae): Possible effects on conservation genetics

Davies

Takashino

Watanabe

et al. 2009

Appl. Entomol. Zool.

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The effects of natural enemy releases on conservation genetics within ecosystems are rarely considered. Diadegma semiclausum (Hellen) (Hymenoptera: Ichneumonidae) was introduced and continues to be released for biological control of diamondback moth in Japan. Diadegma semiclausum and indigenous Diadegma fenestrale (Holmgren) (Hymenoptera: Ichneumonidae) share geographic ranges and hosts, and produce offspring when mated under laboratory conditions. We used DNA to examine whether offspring from inter-specific one-way parental crosses (D. semiclausum / and D. fenestrale ?) were hybrid, as some Hymenoptera (e.g. Aphidius colemani Viereck (Hymenoptera: Braconidae)) exhibit thelytokous reproduction by gynogenesis. Molecular analyses revealed offspring mtDNA (COI) is maternally inherited, as expected, but rRNA (ITS-2) originates from both parents. Should similar hybridization occur in the field beyond the F1 generation, genetic mixing is a possible consequence that may influence biological control efficacy or pollute native population genetics.

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“…To simplify the Trichogramma species identification, several methods have been proposed: (1) Allozymic analysis (Pintureau and Babault, 1980, 1981, 1982Pintureau and Keita, 1989;Kazmer, 1991;Pinto et al, 1992Pinto et al, , 1993Pintureau, 1993). Esterase electrophoresis has provided consistent results for differentiation of some Trichogramma species (Pinto et al, 1992Silva et al, 1999).…”

Section: Introductionmentioning

confidence: 99%

ITS-2 sequences-based identification of Trichogramma species in South America

Almeida

Stouthamer²

2015

Braz. J. Biol.

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ITS2 (Internal transcribed spacer 2) sequences have been used in systematic studies and proved to be useful in providing a reliable identification of Trichogramma species. DNAr sequences ranged in size from 379 to 632 bp. In eleven T. pretiosum lines Wolbachia-induced parthenogenesis was found for the first time. These thelytokous lines were collected in Peru (9), Colombia (1) and USA (1). A dichotomous key for species identification was built based on the size of the ITS2 PCR product and restriction analysis using three endonucleases (EcoRI, MseI and MaeI). This molecular technique was successfully used to distinguish among seventeen native/introduced Trichogramma species collected in South America.Keywords: molecular markers, ITS-2 sequence, Hymenoptera, egg parasitoid. Identificação molecular de espécies de Trichogramma na América do Sul via sequenciamento da região ITS-2 ResumoSequências do Espaço Transcrito Interno 2 (ITS2) têm sido utilizadas em estudos taxonômicos e sua utilidade constatada pela confiabilidade que o método confere à identificação das espécies de Trichogramma. Esta técnica molecular foi bem sucedida em distinguir dezessete espécies nativas e introduzidas de Trichogramma, coletadas na América do Sul. As sequências do DNAr variaram de 379 a 632 pb. Em 11 linhagens de T. pretiosum estudadas, o endosinbionte Wolbachia foi detectado pela primeira vez. Estas linhagens telítocas foram encontradas no Peru (9), Colômbia (1) e Estados Unidos (1). Uma chave dicotômica para identificação de espécies foi construída baseada no tamanho do produto da PCR do ITS2 e em análises de restrição utilizando-se três endonucleases (EcoRI, MseI and MaeI).Palavras-chave: marcadores moleculares, sequências ITS-2, Hymenoptera, parasitóide de ovos.

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Caractérisation enzymatique deTrichogramma evanescens et deT. Maidis [Hym.: Trichogrammatidae]; Étude des hybrides

Cited by 36 publications

References 9 publications

Cytogenetic mechanism and genetic consequences of thelytoky in the wasp Trichogramma cacoeciae

Cytogenetic mechanism and genetic consequences of thelytoky in the wasp Trichogramma cacoeciae

Parental genetic traits in offspring from inter-specific crosses between introduced and indigenous Diadegma Foerster (Hymenoptera: Ichneumonidae): Possible effects on conservation genetics

ITS-2 sequences-based identification of Trichogramma species in South America

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