Caput Epididymidal Mouse Sperm Support Full Development

Zhou, Dongjie; Suzuki, Toru; Asami, Maki; Perry, Anthony C.F.

doi:10.1016/j.devcel.2019.05.012

Cited by 38 publications

(33 citation statements)

References 11 publications

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“…In this context, the findings of Conine et al (2018) suggest that the ability of sperm to support full-term embryo development after ICSI is acquired in the testis, then lost in the caput epididymis, and re-acquired during epididymal transit prior to arriving at the cauda. However, this hypothesis contradicts the results reported by others showing that ICSI produces normal offspring using caput sperm (Suganuma et al, 2005;Zhou et al, 2019).…”

Section: Introductioncontrasting

confidence: 79%

“…There are several methodological differences between Conine et al (2018) and previous studies (Suganuma et al, 2005;Zhou et al, 2019) which could potentially account for the discrepancy about the ability of caput-derived embryos to implant. In our experiments we used similar protocols to Conine et al (2018) with the exception of the mouse strain background (we use B6D2 hybrids and inbred B6, and Conine et al (2018) use inbred FVB).…”

Section: Discussionmentioning

confidence: 95%

“…Further, another two procedures designed to separate the sperm head from tail have been used to produce normal offspring from caput sperm (Suganuma et al, 2005;Zhou et al, 2019). The sperm head can be separated from the midpiece and tail by applying one or a few piezo pulses (Suganuma et al, 2005) or through sonication or the use of detergents like Triton X-100 or 3-[(3-cholamidopropyl) dimethylammonio]-1propanesulfonate (Zhou et al, 2019).…”

Section: Discussionmentioning

confidence: 99%

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Successful ICSI in Mice Using Caput Epididymal Spermatozoa

Fernández‐González

Laguna

Ramos‐Ibeas

et al. 2019

Front. Cell Dev. Biol.

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Spermatozoa undergo their last phase of spermiogenesis, known as maturation, as they pass through the epididymis. A recent report indicates that mouse immature spermatozoa retrieved from the caput epididymis for intracytoplasmic sperm injection (ICSI) give rise to embryos with multiple developmental defects. Further, these embryos were unable to develop to term after their transfer to surrogate mothers. Herein, we examined the potential of mouse caput spermatozoa to produce normal embryos by comparing the use of caput vs. cauda epididymal spermatozoa for in vitro fertilization (IVF) or ICSI. Two methods for the separation of sperm heads prior to ICSI were also compared: freezing/thawing or drawing through a syringe. We found that in contrast to caudal spermatozoa, caput spermatozoa failed to produce embryos via IVF, confirming their immature state. However, regardless of the method employed for the separation of sperm heads, similar efficiencies of blastocyst production in vitro and development to term after transfer to surrogate mothers were observed following ICSI using both caput and cauda epididymal spermatozoa. It therefore seems that mice spermatozoa recovered from the caput epididymis are as valid as caudal spermatozoa for the production of embryos and offspring by ICSI.

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Section: Introductioncontrasting

confidence: 79%

Section: Discussionmentioning

confidence: 95%

Section: Discussionmentioning

confidence: 99%

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Successful ICSI in Mice Using Caput Epididymal Spermatozoa

Fernández‐González

Laguna

Ramos‐Ibeas

et al. 2019

Front. Cell Dev. Biol.

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“…These results were attributed to non-coding, small RNA (sRNA) payloads in caput sperm, as these payloads undergo significant remodeling during epididymal transit [43][44][45]. However, some data from other groups are in disagreement with these results, where authors successfully generated ICSI offspring using caput sperm [46,47]. Even considering inconsistencies in results, both historical and recent data suggest sperm competency to support normal embryogenesis may be acquired during epididymal transit.…”

Section: Sperm Maturation and Reproductionmentioning

confidence: 99%

“…Contrasting observations have been made, however, where offspring could be successfully generated using ICSI with caput sperm [46,47]. Multiple explanations for these conflicting results have been proposed, including minor differences in methods and the use of different mouse strains [43].…”

Section: Support Of Embryogenesismentioning

confidence: 99%

The Role of the Epididymis and the Contribution of Epididymosomes to Mammalian Reproduction

James

Carrell

Aston

et al. 2020

IJMS

172

103

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It is well-established that testicular spermatozoa are immature and acquire motility and fertilization capabilities during transit throughout the epididymis. The epididymis is a duct-like organ that connects the testis to the vas deferens and is comprised of four anatomical regions: the initial segment, caput, corpus, and cauda. Sperm maturation occurs during epididymal transit by the interaction of sperm cells with the unique luminal environment of each epididymal region. In this review we discuss the epididymis as an essential reproductive organ responsible for sperm concentration, maturation (including sperm motility acquisition and fertilizing ability), protection and storage. Importantly, we also discuss specific characteristics and roles of epididymal-derived exosomes (epididymosomes) in establishing sperm competency within the intricate process of reproduction. This review suggests that an increasing body of evidence is working to develop a complete picture of the role of the epididymis in male reproduction, offspring health, and disease susceptibility.

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Inheritance and maintenance of small RNA‐mediated epigenetic effects

et al. 2022

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Heritable traits are predominantly encoded within genomic DNA, but it is now appreciated that epigenetic information is also inherited through DNA methylation, histone modifications, and small RNAs. Several examples of transgenerational epigenetic inheritance of traits have been documented in plants and animals. These include even the inheritance of traits acquired through the soma during the life of an organism, implicating the transfer of epigenetic information via the germline to the next generation. Small RNAs appear to play a significant role in carrying epigenetic information across generations. This review focuses on how epigenetic information in the form of small RNAs is transmitted from the germline to the embryos through the gametes. We also consider how inherited epigenetic information is maintained across generations in a small RNA-dependent and independent manner. Finally, we discuss how epigenetic traits acquired from the soma can be inherited through small RNAs.

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Caput Epididymidal Mouse Sperm Support Full Development

Cited by 38 publications

References 11 publications

Successful ICSI in Mice Using Caput Epididymal Spermatozoa

Successful ICSI in Mice Using Caput Epididymal Spermatozoa

The Role of the Epididymis and the Contribution of Epididymosomes to Mammalian Reproduction

Inheritance and maintenance of small RNA‐mediated epigenetic effects

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