Capturing Single Molecules of Immunoglobulin and Ricin with an Aptamer-Encoded Glass Nanopore

Ding, Shu Li; Gao, Changlu; Gu, Li-Qun

doi:10.1021/ac9006705

Cited by 142 publications

(125 citation statements)

References 73 publications

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“…The glass surface can also be modified with analyte-specific binding molecules (e.g. an antibody or a DNA aptamer) to selectively capture and detect analyte molecules with high selectivity and sensitivity [35,36] and with chemical functionalities that respond to external stimuli (e.g. pH or light) [37,38].…”

Section: (B) Surface Modificationmentioning

confidence: 99%

Resistive-pulse and rectification sensing with glass and carbon nanopipettes

Wang

Mirkin

2017

Proc. R. Soc. A.

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Along with more prevalent solid-state nanopores, glass or quartz nanopipettes have found applications in resistive-pulse and rectification sensing. Their advantages include the ease of fabrication, small physical size and needle-like geometry, rendering them useful for local measurements in small spaces and delivery of nanoparticles/biomolecules. Carbon nanopipettes fabricated by depositing a thin carbon layer on the inner wall of a quartz pipette provide additional means for detecting electroactive species and fine-tuning the current rectification properties. In this paper, we discuss the fundamentals of resistivepulse sensing with nanopipettes and our recent studies of current rectification in carbon pipettes.

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Section: (B) Surface Modificationmentioning

confidence: 99%

Resistive-pulse and rectification sensing with glass and carbon nanopipettes

Wang

Mirkin

2017

Proc. R. Soc. A.

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“…Other surfaces, such as silicon oxide 9 or alumina 23 may also be drilled using this technique, however the number of thin films amenable to this technique is limited to those that can be manufactured so that they are free-standing, thin and free of holes. To overcome this issue, chemical coating of the nanopore may be used 23,[38][39][40] . Another trade off of the single nanopore technique is that it can only look at one molecule at a time.…”

Section: Representative Resultsmentioning

confidence: 99%

Monitoring Protein Adsorption with Solid-state Nanopores

Niedzwiecki

Movileanu

2011

JoVE

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Solid-state nanopores have been used to perform measurements at the single-molecule level to examine the local structure and flexibility of nucleic acids [1][2][3][4][5][6] , the unfolding of proteins 7 , and binding affinity of different ligands 8 . By coupling these nanopores to the resistive-pulse technique [9][10][11][12] , such measurements can be done under a wide variety of conditions and without the need for labeling 3 . In the resistive-pulse technique, an ionic salt solution is introduced on both sides of the nanopore. Therefore, ions are driven from one side of the chamber to the other by an applied transmembrane potential, resulting in a steady current. The partitioning of an analyte into the nanopore causes a well-defined deflection in this current, which can be analyzed to extract single-molecule information. Using this technique, the adsorption of single proteins to the nanopore walls can be monitored under a wide range of conditions 13 . Protein adsorption is growing in importance, because as microfluidic devices shrink in size, the interaction of these systems with single proteins becomes a concern. This protocol describes a rapid assay for protein binding to nitride films, which can readily be extended to other thin films amenable to nanopore drilling, or to functionalized nitride surfaces. A variety of proteins may be explored under a wide range of solutions and denaturing conditions. Additionally, this protocol may be used to explore more basic problems using nanopore spectroscopy. Video LinkThe video component of this article can be found at http://www.jove.com/video/3560/ Protocol 1. Manufacture of solid-state nanopores in silicon nitride membranes 1. Bring the FEI Tecnai F20 S/TEM to an acceleration voltage of 200 kV. If using a different S/TEM, the acceleration voltage should be greater than or equal to 200 kV 9 2. Load a 20 nm thick SPI silicon nitride window grid into the TEM sample holder and clean with Oxygen plasma for 30 seconds to remove any contaminants from the holder. 3. Load the sample into the S/TEM and allow for the vacuum to pump down. Once the S/TEM has pumped down to vacuum, find the nitride window in bright-field TEM mode by looking for a bright square on the Ronchigram. Make sure the TEM is aligned properly, then align and focus the sample. 4. Switch the S/TEM into STEM mode. Use the HAADF (or equivalent) detector to image the sample and make sure it is properly aligned. 5. Set the Monochromator to a low value. A lower value of the Monochromator allows for a higher current of electrons available for drilling. If the S/TEM does not have a Monochromator, ignore this step. 6. Select an appropriate spot size for drilling the nanopore. This corresponds to the diameter of the electron probe. A 3 nm probe works well for drilling 5 nm diameter pores. A larger probe (5 nm) will drill more quickly and create a larger pore. A smaller probe (1 nm) will take longer to drill and create a smaller pore. 7. Adjust the STEM alignments, if necessary. 8. Focus the nitride membrane a...

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“…Relevant references that are related to our approach are those of synthetic nanopore membranes, which detect biomolecules such as proteins [107][108][109][110] and DNA [111][112][113][114] by plugging the nanotube with the analyte, and that of mesoporous silica with a polyamine derivative as a molecular gate, which selectively recognizes ions such as ATP 4-and blocks the release of an optical marker. 115,116 The versatility of the approaches, including ours, suggest that robust channel systems are promissing for creating sensitive biosensing methods.…”

Section: ·3 Solid-phase Fluorometric Immunoassaymentioning

confidence: 99%

Pore-forming Compounds as Signal Transduction Elements for Highly Sensitive Biosensing

2014

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119 IntroductionTranslocation of ions and small molecules across barriers of cellular membrane, lipid bilayers and solid supports is elicited by biological and artificial pore-forming compounds, which comprise protein, peptide and artificial molecules. Application of nanopores to biosensing holds much promise in developing highly sensitive methods because of their inherent ability of signal transduction and amplification.Nanopore-forming compounds, such as receptor ion-channels, channel-forming peptides and synthetic channels embedded in spherical and planar lipid bilayers ( Fig. 1), have been exploited as sensing elements for the development of biosensors and their uses for biosensing have been reviewed extensively. 1-13Receptor ionchannels, such as glutamate receptor ion channels, possess a 2014 © The Japan Society for Analytical Chemistry Pharmacy and Life Science, 1432-1 Horinouchi, Hachioji, Tokyo 192-0392, Japan Pore-forming compounds are attracting much attention due to the signal transduction ability for the development of highly sensitive biosensing. In this review, we describe an overview of the recent advances made by our group in the design of molecular sensing interfaces of spherical and planar lipid bilayers and natural bilayers. The potential uses of poreforming compounds, such as gramicidin and MCM-41, in lipid bilayers and natural glutamate receptor channels in biomembrane are presented.

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Capturing Single Molecules of Immunoglobulin and Ricin with an Aptamer-Encoded Glass Nanopore

Cited by 142 publications

References 73 publications

Resistive-pulse and rectification sensing with glass and carbon nanopipettes

Resistive-pulse and rectification sensing with glass and carbon nanopipettes

Monitoring Protein Adsorption with Solid-state Nanopores

Pore-forming Compounds as Signal Transduction Elements for Highly Sensitive Biosensing

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