Capturing excited-state structural snapshots of evolutionary green-to-red photochromic fluorescent proteins
Taylor D. Krueger,
J. Nathan Henderson,
Isabella L. Breen
et al.
Abstract:Photochromic fluorescent proteins (FPs) have proved to be indispensable luminous probes for sophisticated and advanced bioimaging techniques. Among them, an interplay between photoswitching and photoconversion has only been observed in a limited subset of Kaede-like FPs that show potential for discovering the key mechanistic steps during green-to-red photoconversion. Various spectroscopic techniques including femtosecond stimulated Raman spectroscopy (FSRS), X-ray crystallography, and femtosecond transient abs… Show more
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