Capturing biodiversity: linking a cyanobacteria culture collection to the “scratchpads” virtual research environment enhances biodiversity knowledge

Gkelis, Spyros; Panou, Manthos

doi:10.3897/bdj.4.e7965

Cited by 30 publications

(16 citation statements)

References 15 publications

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“…Monospecific cultures were isolated and further purified by successive transfers and using antibiotics (such as cycloheximide and ampicillin) as described in Rippka [ 33 ]. The isolates were deposited in Aristotle University of Thessaloniki Microalgae and Cyanobacteria Collection (TAU-MAC) [ 34 ] and can be accessed at http://cyanobacteria.myspecies.info/ .…”

Section: Methodsmentioning

confidence: 99%

Sponges-Cyanobacteria associations: Global diversity overview and new data from the Eastern Mediterranean

et al. 2018

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Sponge-cyanobacteria associations have attracted research interest from an ecological, evolutionary and biotechnological perspective. Current knowledge is, in its majority, “hidden” in metagenomics research studying the entire microbial communities of sponges, while knowledge on these associations is totally missing for certain geographic areas. In this study, we (a) investigated the occurrence of cyanobacteria in 18 sponge species, several of which are studied for the first time for their cyanobionts, from a previously unexplored eastern Mediterranean ecoregion, the Aegean Sea, (b) isolated sponge-associated cyanobacteria, and characterized them based on a polyphasic (morphological-morphometric and molecular phylogenetic analysis) approach, and (c) conducted a meta-analysis on the global diversity of sponge species hosting cyanobacteria, as well as the diversity of cyanobacterial symbionts. Our research provided new records for nine sponge species, previously unknown for this association, while the isolated cyanobacteria were found to form novel clades within Synechococcus, Leptolyngbyaceae, Pseudanabaenaceae, and Schizotrichaceae, whose taxonomic status requires further investigation; this is the first report of a Schizotrichaceae cyanobacterium associated with sponges. The extensive evaluation of the literature along with the new data from the Aegean Sea raised the number of sponge species known for hosting cyanobacteria to 320 and showed that the cyanobacterial diversity reported from sponges is yet underestimated.

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Section: Methodsmentioning

confidence: 99%

Sponges-Cyanobacteria associations: Global diversity overview and new data from the Eastern Mediterranean

et al. 2018

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“…The recent research on cyanobacterial diversity in Greece comprises polyphasic or multidisciplinary studies of cyanobacteria strains (Gkelis et al 2005b, Lamprinou et al 2011, Lamprinou et al 2013a, Lamprinou et al 2013b, Gkelis et al 2015b, Gkelis and Panou 2016, Bravakos et al 2016), natural populations (Moustaka-Gouni et al 2009, Moustaka-Gouni et al 2010, Papadimitriou et al 2013, Gkelis and Zaoutsos 2014, Gkelis et al 2014, Gkelis et al 2015a), as well as ecological works (e.g. Lamprinou et al 2009, Katsiapi et al 2011, Katsiapi et al 2012).…”

Section: Introductionmentioning

confidence: 99%

Cyanobacteria of Greece: an annotated checklist

Gkelis¹,

Ourailidis²,

Panou³

et al. 2016

BDJ

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BackgroundThe checklist of Greek Cyanobacteria was created in the framework of the Greek Taxon Information System (GTIS), an initiative of the LifeWatchGreece Research Infrastructure (ESFRI) that has resumed efforts to compile a complete checklist of species reported from Greece. This list was created from exhaustive search of the scientific literature of the last 60 years. All records of taxa known to occur in Greece were taxonomically updated.New informationThe checklist of Greek Cyanobacteria comprises 543 species, classified in 130 genera, 41 families, and 8 orders. The orders Synechococcales and Oscillatoriales have the highest number of species (158 and 153 species, respectively), whereas these two orders along with Nostocales and Chroococcales cover 93% of the known Greek cyanobacteria species. It is worth mentioning that 18 species have been initially described from Greek habitats. The marine epilithic Ammatoidea aegea described from Saronikos Gulf is considered endemic to this area. Our bibliographic review shows that Greece hosts a high diversity of cyanobacteria, suggesting that the Mediterranean area is also a hot spot for microbes.

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“…A strain of the TAU-MAC culture collection (Gkelis and Panou, 2016), Microcystis flos-aquae TAU-MAC 1510, isolated from Lake Pamvotis, Greece (Gkelis et al, 2015a), was used for experimental purposes. Microcystis flos-aquae TAU-MAC 1510 has been found to be toxic (Gkelis et al, 2015a), producing a range of microcystins, including MC-YR, MC-LR, [D-Asp 3 ] MC-LR and MC-HilR (Gkelis et al, 2019).…”

Section: Methodsmentioning

confidence: 99%

The effects of microcystin-LR inOryza sativaroot cells: F-actin as a new target of cyanobacterial toxicity

Pappas

Gkelis

Panteris

2020

Preprint

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ABSTRACTMicrocystins are toxins produced by cyanobacteria, notorious for negatively affecting a wide range of living organisms, among which several plant species. Although microtubules are a well-established target of microcystin toxicity, its effect on filamentous actin (F-actin) in plant cells has not been studied yet.The effects of microcystin-LR (MC-LR) and the extract of a microcystin-producing freshwater cyanobacterial strain (Microcystis flos-aquae TAU-MAC 1510) on the cytoskeleton (F-actin and microtubules) of Oryza sativa (rice) root cells, were studied by light, confocal, and transmission electron microscopy. Considering the role of F-actin in endomembrane system distribution, the endoplasmic reticulum and the Golgi apparatus in extract-treated cells were also examined.F-actin in both MC-LR- and extract-treated meristematic and differentiating root cells exhibited time-dependent alterations, ranging from disorientation and bundling to the formation of ring-like structures, eventually resulting to a collapse of the F-actin network at longer treatments. Disorganization and eventual depolymerization of microtubules, as well as abnormal chromatin condensation were observed following treatment with the extract, effects which could be attributed to microcystins and other bioactive compounds. Moreover, cell cycle progression was inhibited in extract-treated roots, specifically affecting the mitotic events. As a consequence of F-actin network disorganization, endoplasmic reticulum elements appeared stacked and diminished, while Golgi dictyosomes appeared aggregated.These results support that F-actin is a prominent target of MC-LR, both in pure form and as an extract ingredient. Endomembrane system alterations can also be attributed to the effects of cyanobacterial bioactive compounds (including microcystins) on F-actin cytoskeleton.

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Capturing biodiversity: linking a cyanobacteria culture collection to the “scratchpads” virtual research environment enhances biodiversity knowledge

Cited by 30 publications

References 15 publications

Sponges-Cyanobacteria associations: Global diversity overview and new data from the Eastern Mediterranean

Sponges-Cyanobacteria associations: Global diversity overview and new data from the Eastern Mediterranean

Cyanobacteria of Greece: an annotated checklist

The effects of microcystin-LR inOryza sativaroot cells: F-actin as a new target of cyanobacterial toxicity

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