Capturing and recovering of Cryptosporidium parvum oocysts with polymeric micro-fabricated filter

Recovery ofCryptosporidiumandGiardiaorganisms from surface water by counter-flow refining microfiltration

“…This water-particle refining ability of the CFMR may help to lower the turbidity level for the final concentrates, which is crucial to improve the recovery. [24,30] Multiple μC units were utilized for the refining filter in an effort to extend the effective filtration area for the counterflow concentrator. Thus, the design allows filtration of large water volumes at high flow rates, which would be rather complicated with the use of a single μC.…”

“…[18,19] In particular, promising filtration solutions based on silicon microfabrication technology for microbial cell concentration have been proposed during the last two decades, including microfabricated membranes and cross-flow microfilters. [20][21][22][23] Warkiani et al [24] developed a silicon-based microfilter to concentrate C. parvum oocysts, reporting higher recovery efficiency compared with conventional concentration techniques. High water flux, large effective filtration area and low clogging ratio were also demonstrated for the microfabrication filter systems.…”

Fabrication of multi-layer polymeric micro-sieve having narrow slot pores with conventional ultraviolet-lithography and micro-fabrication techniques

“…The pore density of polymeric microfabricated membrane is much higher than the commercial filter like polymeric track-etched membrane with CV and average pore density of around 20% and 10 7 pores/cm 2 , respectively. 20,22 The histogram of analyzed samples is depicted schematically in Figure 5. Figure 6 illustrates the filtrate collection volume data of the micro-fabricated multi-layer membrane and a track-etched polycarbonate membrane (Millipore, pore size of 3 lm, Cat.…”

“…Unique features of the multi-layer polymeric micro-sieve like the smooth surface and uniform pore-size greatly reduce the oocyst adhesion to the filter surface and enable us to achieve a very high recovery rate in comparison to the available commercial filters for this purpose. 23,24 D. Reusability and other applications Commercial micro-filters are not designed to be reusable, and they can hardly retain their initial status after the filtration and recovery process 20 (i.e., many cells trap inside their tortuous pore path) while the polymeric multi-layer micro-sieve can easily retain its original condition with a simple back-flushing or lateral shaking. SU-8 also has good chemical properties and can be cleaned using appropriate buffers or chemical like weak acids.…”

Three-dimensional hydraulic modeling of particle deposition on the patterned isopore membrane in crossflow microfiltration

“…Recently, studies to reduce pore defects have been carried out by applying self-assembly, anodization, and microelectochemical system (MEMS) to isopore membrane fabrication processes [17,18]. The increased selectivity by ordered pore structure enabled enhanced separation of specific microorganism [19,20].…”