Capture of cervical exfoliative cells on a glass slide coated by 3-glycidyloxypropyl trimethoxysilane and poly-L-lysine

Xing, Gaowa; Xiang, Sen; Xue, Wei; Gao-wa, Aodeng; Liu, Yan; Zhang, Jinghua; Lin, Jin‐Ming

doi:10.1016/j.jpha.2012.02.008

Cited by 9 publications

(10 citation statements)

References 30 publications

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“…Of note, the roughness of the SiC surface (rms: 1.66 nm) increased after silanization (rms: 2.52 nm). Xing et al . reported that the liquid deposition of GOPTS on glass did indeed increase the roughness of the substrate but likewise also the number of adhesive cells grown on top of this monolayer; our results are aligned with this finding.…”

Section: Resultssupporting

confidence: 91%

Functional Microarray Platform with Self-Assembled Monolayers on 3C-Silicon Carbide

et al. 2020

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Currently available bio-platforms such as microarrays and surface plasmon resonators are unable to combine high throughput multiplexing with label-free detection. As such, emerging Micro-Electro-Mechanical Systems (MEMS) and microplasmonics platforms offer the potential for high-resolution, high-throughput label-free sensing of biological and chemical analytes. Therefore, the search for materials capable of combining multiplexing and label free quantitation is of great significance. Recently, interest in silicon carbide (SiC) as a suitable material in numerous biomedical applications has increased due to its well explored chemical inertness, mechanical strength, bio-and hemo-compatibility and the presence of carbon that enables transfer free growth of graphene. SiC is also multifunctional as both a wide band gap semiconductor and an efficient low-loss plasmonics material, and thus is ideal for augmenting current biotransducers in biosensors. Additionally, the cubic variant, 3C-SiC, is an extremely promising material for MEMS, being a suitable platform for the easy micromachining of microcantilevers, it is capable of realizing the potential of real time miniaturized multiplexed assays. However, to realize the full potential of SiC for label free, multiplexed quantitation of biological interactions the generation of an appropriately functionalized and versatile organic monolayer suitable for the immobilization of biomolecules is critical. Herein, we address the use of various silane self-assembled monolayers (SAMs) for covalent functionalization of monocrystalline 3C-SiC films as a novel platform for the generation of functionalized microarray surfaces using high-throughput glycan arrays as the model system. We also demonstrate the ability to robotically print high throughput arrays on free standing SiC microstructures. The implementation of a SiC-based label free glycan array will provide proof-of-principle that could be extended to the immobilization of other biomolecules in a similar SiC-based array format, thus making potentially significant advances to the way biological interactions are studied.

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Section: Resultssupporting

confidence: 91%

Functional Microarray Platform with Self-Assembled Monolayers on 3C-Silicon Carbide

et al. 2020

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“…This work provides an efficient method for the preparation of organic mixed ionic–electronic conductor films stable in aqueous environments that does not compromise the electronic properties. While this work focused on PEDOT:PSS on various substrates (glass, silicon wafer, gold), the same functionalization method could be extended to other organic semiconductors containing nucleophilic substituents that can react with epoxides, such as carboxyl, and amine , functional groups, to reduce substrate delamination and increase the mechanical stability of the device for bioelectronics applications in aqueous environments.…”

Section: Introductionmentioning

confidence: 99%

“…Instead of blending GOPS with PEDOT:PSS, only the surface of the substrate is functionalized with GOPS to introduce a silane monolayer with epoxy groups before spin-coating PEDOT:PSS (Figure 1c). GOPS is commonly utilized in biomedical applications as an adhesive monolayer for the immobilization of proteins, 38 DNA, 39 and cervical exfoliative cells, 40 but these monolayers had surprisingly never been explored for the deposition of PEDOT:PSS. Films obtained by surface functionalization were compared to those obtained by blending and showed similar operational stability in water under passive conditions and electrochemical cycling.…”

Section: ■ Introductionmentioning

confidence: 99%

Surface Functionalization with (3-Glycidyloxypropyl)trimethoxysilane (GOPS) as an Alternative to Blending for Enhancing the Aqueous Stability and Electronic Performance of PEDOT:PSS Thin Films

Osazuwa,

Lo,

Feng

et al. 2023

ACS Appl. Mater. Interfaces

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Organic mixed ionic−electronic conductors, such as poly (3,4ethylenedioxythiophene):poly(styrenesulfonate) (PEDOT:PSS), are essential materials for the fabrication of bioelectronic devices due to their unique ability to couple and transport ionic and electronic charges. The growing interest in bioelectronic devices has led to the development of organic electrochemical transistors (OECTs) that can operate in aqueous solutions and transduce ionic signals of biological origin into measurable electronic signals. A common challenge with OECTs is maintaining the stability and performance of the PEDOT:PSS films operating under aqueous conditions. Although the conventional approach of blending the PEDOT:PSS dispersions with a cross-linker such as (3glycidyloxypropyl)trimethoxysilane (GOPS) helps to ensure strong adhesion of the films to device substrates, it also impacts the morphology and thus electrical properties of the PEDOT:PSS films, which leads to a significant reduction in the performance of OECTs. In this study, we instead functionalize only the surface of the device substrates with GOPS to introduce a silane monolayer before spin-coating the PEDOT:PSS dispersion on the substrate. In all cases, having a GOPS monolayer instead of a blend leads to increased electronic performance metrics, such as three times higher electronic conductivity, volumetric capacitance, and mobility− capacitance product [μC*] value in OECT devices, ultimately leading to a record value of 406 ± 39 F cm −1 V −1 s −1 for amorphous PEDOT:PSS. This increased performance does not come at the expense of operational stability, as both the blend and surface functionalization show similar performance when subjected to pulsed gate bias stress, long-term electrochemical cycling tests, and aging over 150 days. Overall, this study establishes a novel approach to using GOPS as a surface monolayer instead of a blended cross-linker, for achieving high-performance organic mixed ionic−electronic conductors that are stable in water for bioelectronics.

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“…In the culture system, the most easily adherent cells are fibroblasts, followed by glial cells, and finally neurons 22 . PLL encapsulation can improve the adhesion ability of cells 23,24 . Therefore, we assume that PLL has different adhesion ability to different cells and will separate fibroblasts and other heterocytic cells.…”

Section: Discussionmentioning

confidence: 99%

Study of the method of spinal cord neuron culture in Sprague–Dawley rats

Sun

Chang

Eerqing

et al. 2022

Ibrain

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This study aimed to explore the method of culture of spinal cord neurons (SPNs) in vitro and to provide prerequisites for studying the molecular mechanism and pharmacological mechanism of spinal cord injury and repair. The spinal cord tissues of neonatal Sprague–Dawley rats were taken and digested by trypsin, followed by cytarabine (Ara‐C) to inhibit the proliferation of heterogeneous cells, differential velocity adhesion, and natural growth in neuron‐specific medium. Then, the morphology of SPNs was observed. Ara‐C treatment inhibited the growth of heterogeneous cells and the growth of spinal neurons. Using the differential velocity adhesion method, it was found that the adhesion time of heterogeneous cells and SPNs was not significantly different, and it could not separate neurons and heterogeneous cells well. A large number of mixed cells gathered and floated, and died on the 18th day. Compared with the 20th day, the cell viability of the 18th day was better (p < 0.001). The natural growth and culture of SPNs in Neurobasal‐A medium can yield neurons of higher purity and SPNs from the 12th day to the 18th day can be selected for related in vitro cell experiments.

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Capture of cervical exfoliative cells on a glass slide coated by 3-glycidyloxypropyl trimethoxysilane and poly-L-lysine

Cited by 9 publications

References 30 publications

Functional Microarray Platform with Self-Assembled Monolayers on 3C-Silicon Carbide

Functional Microarray Platform with Self-Assembled Monolayers on 3C-Silicon Carbide

Surface Functionalization with (3-Glycidyloxypropyl)trimethoxysilane (GOPS) as an Alternative to Blending for Enhancing the Aqueous Stability and Electronic Performance of PEDOT:PSS Thin Films

Study of the method of spinal cord neuron culture in Sprague–Dawley rats

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