Captopril, a specific inhibitor of angiotensin converting enzyme, enhances both trypsin and vitellogenin titers in the grey fleshfly<i>Neobellieria bullata</i>

Vandingenen, Anick; Hens, Korneel; Macours, Nathalie; Zhu, Wei; Janssen, Ine; Breuer, Michael; Loof, Arnold De; Huybrechts, Roger

doi:10.1002/arch.1047

Cited by 21 publications

(25 citation statements)

References 24 publications

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“…Since Neb-TMOF is an in vivo substrate for Neb-ACE, the captopril treatment had a direct effect on trypsin activity and vitellogenin concentrations. Vandingenen et al (2001) also reported that captopril fed to female flies caused an increase in the liver meal-induced trypsin peak in the midgut and elevated levels of protein-induced yolk polypeptides in the hemolymph, but oocyte growth was not affected.…”

Section: Introductionmentioning

confidence: 93%

“…Females that had been mated with these ACE-inhibited males showed a dramatic reduction in fecundity (Ekbote et al, 2003a). In addition, Vandingenen et al (2001Vandingenen et al ( , 2002 treated female adults of the grey fleshfly Neobellieria bullata with captopril and studied the in vivo relationship between Neb-TMOF (trypsin modulating oostatic factor) and Neb-ACE. Since Neb-TMOF is an in vivo substrate for Neb-ACE, the captopril treatment had a direct effect on trypsin activity and vitellogenin concentrations.…”

Section: Introductionmentioning

confidence: 99%

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The angiotensin converting enzyme inhibitor captopril reduces oviposition and ecdysteroid levels in Lepidoptera

Vercruysse

Gelman

Raes

et al. 2004

Arch Insect Biochem Physiol

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The role of angiotensin converting enzyme (ACE, peptidyl dipeptidase A) in metamorphic- and reproductive-related events in the Egyptian cotton leafworm, Spodoptera littoralis (Lepidoptera, Noctuidae) was studied by using the selective ACE inhibitor captopril. Although oral administration of captopril had no effect on larval growth, topical administration to new pupae resulted in a large decrease of successful adult formation. Oviposition and overall appearance of adults emerging from treated larvae did not differ significantly from those emerging from non-treated larvae. In contrast, topical or oral administration of captopril to newly emerged adults caused a reduction in oviposition. By evaluating the effect of captopril on ecdysteroid titers and trypsin activity, we revealed an additional physiological role for ACE. Captopril exerted an inhibitory effect on ecdysteroid levels in female but not in male adults. Larvae fed a diet containing captopril exhibited increased trypsin activity. A similar captopril-induced increase in trypsin activity was observed in female adults. In male adults, however, captopril elicited reduced levels of trypsin activity. Our results suggest that captopril downregulates oviposition by two independent pathways, one through ecdysteroid biosynthesis regulation, and the other through regulation of trypsin activity. Apparently, fecundity is influenced by a complex interaction of ACE, trypsin activity, and ecdysteroid levels.

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Section: Introductionmentioning

confidence: 93%

Section: Introductionmentioning

confidence: 99%

The angiotensin converting enzyme inhibitor captopril reduces oviposition and ecdysteroid levels in Lepidoptera

Vercruysse

Gelman

Raes

et al. 2004

Arch Insect Biochem Physiol

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“…These results are in agreement with the general tissue distribu tion of ACE in insects. Although this distribution is not identical in different species, ACE seems to be concen trated in certain tissues, including reproductive tissues, brain, midgut and hemolymph (Cornell et al, 1995;Ekbote et al, 1999;Vandingenen et al, 2001;Vandingenen et al, 2002), suggesting that the enzyme is of physiological importance in these tissues. Indeed, numerous reports substantiate the involvement of insect ACE in reproduction, pro-hormone processing and regu lation of peptide titers (Isaac et al, 1994;Tatei et al, 1995;Wijffels et al, 1996;Lamango et al, 1997).…”

Section: Discussionmentioning

confidence: 98%

“…ACE activity measurements were based on the method pro posed by Ryan et al (1977) and modified as in Vandingenen et al (2001). Typically, reactions were conducted in a final volume of 100 pl in HEPES (see above) buffer.…”

Section: Ace Activity Assaymentioning

confidence: 99%

“…Although we have indicated that the Neobellieria bullata trypsin modulating oostatic factor (NebTMOF) is a putative in vivo substrate for Neb ACE (Vandingenen et al, 2001), the exact physiological relevance of insect ACE remains obscure Veelaert et al, 1999;Seinsche et al, 2000) . Cloning and/or purification of ACE from the fruitfly Drosophila melanogaster (AnCE) (Cornell et al, 1995) , the housefly Musca domestica (Lamango et al, 1996) , the buffalo fly Haematobia irritans exigua (HieACE) (Wijffels et al, 1996), and the silk worm Bombyx mori (BmACER or ACE-related) (Quan et al, 2001) have contributed to the molecular and biochemical characterization of insect ACE, which is composed of only one domain and shares structural and enzymatic properties with tACE and the C-domain of sACE.…”

Section: Introductionmentioning

confidence: 98%

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Isolation of angiotensin converting enzyme from testes of Locusta migratoria (Orthoptera)

Macours¹,

Vandingenen²,

Gielens³

et al. 2003

Eur. J. Entomol.

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Abstract. By means of a tracer assay using a labeled synthetic angiotensin converting enzyme (ACE) substrate hippurylglycylglycine, we have detected high ACE activity in the testes of the African migratory locust, Locusta migratoria. Lower, but significant, ACE activity was observed in midgut and hemolymph. In a two-step purification procedure involving anion exchange and gel permeation chromatography, we have purified LomACE from the locust testes. The enzyme of approximately 80 kDa shows substantial amino-acid sequence homology with ACE from both vertebrate and invertebrate origin. The ACE identity of the purified enzyme was further confirmed by cDNA cloning of the Locusta ACE fragment, which, after in silico translation, revealed a mature protein of 623 amino acids with a large structural similarity to other known ACE proteins.

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Effect of ace inhibitors and TMOF on growth, development, and trypsin activity of larval Spodoptera littoralis

Lemeire

Borovsky

Camp

et al. 2008

Arch Insect Biochem Physiol

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Angiotensin converting enzyme (ACE) is a zinc metallopeptidase capable of cleaving dipeptide or dipeptideamide moieties at the C-terminal end of peptides. ACE is present in the hemolymph and reproductive tissues of insects. The presence of ACE in the hemolymph and its broad substrate specificity suggests an important role in processing of bioactive peptides. This study reports the effects of ACE inhibitors on larval growth in the cotton leafworm Spodoptera littoralis. Feeding ACE inhibitors ad lib decreased the growth rate, inhibited ACE activity in the larval hemolymph, and down-regulated trypsin activity in the larval gut. These results indicate that S. littoralis ACE may influence trypsin biosynthesis in the larval gut by interacting with a trypsin-modulating oostatic factor (TMOF). Injecting third instar larvae with a combination of Aea-TMOF and the ACE inhibitor captopril, down-regulated trypsin biosynthesis in the larval gut indicating that an Aea-TMOF gut receptor analogue could be present. Injecting captopril and enalapril into newly molted fifth instar larvae stopped larval feeding and decreased weight gain. Together, these results indicate that ACE inhibitors are efficacious in stunting larval growth and ACE plays an important role in larval growth and development.

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Captopril, a specific inhibitor of angiotensin converting enzyme, enhances both trypsin and vitellogenin titers in the grey fleshflyNeobellieria bullata

Cited by 21 publications

References 24 publications

The angiotensin converting enzyme inhibitor captopril reduces oviposition and ecdysteroid levels in Lepidoptera

The angiotensin converting enzyme inhibitor captopril reduces oviposition and ecdysteroid levels in Lepidoptera

Isolation of angiotensin converting enzyme from testes of Locusta migratoria (Orthoptera)

Effect of ace inhibitors and TMOF on growth, development, and trypsin activity of larval Spodoptera littoralis

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