Captive maturation studies in Penaeus monodon by GIH silencing using constitutively expressed long hairpin RNA

Das, Rekha; Krishna, Gopal; Priyadarshi, Himanshu; Gireesh‐Babu, P.; Pavan‐Kumar, A.; Rajendran, K. V.; Reddy, A.K.; Makesh, M.; Chaudhari, Aparna

doi:10.1016/j.aquaculture.2015.06.036

Cited by 21 publications

(19 citation statements)

References 33 publications

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“…In this condition the researchers are practically forced to normalize a target gene with any one of the popularly used reference genes. This is very much apparent from most of the literature (Paria et al, 2013; Das et al, 2015; Sharabi et al, 2015). Hence, there is a desperate need for a method, with good sensitivity in both large and small panels to identify a stable reference gene and provide possible correction measures, if no reference gene in the panel exhibits stable expression.…”

Section: Introductionmentioning

confidence: 84%

“…Relative quantification of gene expression has been accepted as the gold standard method in several modern techniques such as quantitative real-time PCR, microarray, and high-throughput sequencing (Grozinger et al, 2007; Wang et al, 2012a; Wang et al, 2012b; Paria et al, 2013; Das et al, 2015; Van Hoeck et al, 2015) whose accuracy depends largely on the stability of the reference gene employed for normalization (Bustin, 2002; Leelatanawit et al, 2012). Ideally, to qualify as stable, a reference gene should express at a constant level in each sample across the groups/categories (treatments, tissues, developmental stages, etc).…”

Section: Introductionmentioning

confidence: 99%

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Analysis of variance, normal quantile-quantile correlation and effective expression support of pooled expression ratio of reference genes for defining expression stability

Priyadarshi¹,

Das

Kumar

et al. 2017

Heliyon

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Identification of a reference gene unaffected by the experimental conditions is obligatory for accurate measurement of gene expression through relative quantification. Most existing methods directly analyze variability in crossing point (Cp) values of reference genes and fail to account for template-independent factors that affect Cp values in their estimates. We describe the use of three simple statistical methods namely analysis of variance (ANOVA), normal quantile-quantile correlation (NQQC) and effective expression support (EES), on pooled expression ratios of reference genes in a panel to overcome this issue. The pooling of expression ratios across the genes in the panel nullify the sample specific effects uniformly affecting all genes that are falsely reflected as instability. Our methods also offer the flexibility to include sample specific PCR efficiencies in estimations, when available, for improved accuracy. Additionally, we describe a correction factor from the ANOVA method to correct the relative fold change of a target gene if no truly stable reference gene could be found in the analyzed panel. The analysis is described on a synthetic data set to simplify the explanation of the statistical treatment of data.

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Section: Introductionmentioning

confidence: 84%

Section: Introductionmentioning

confidence: 99%

Analysis of variance, normal quantile-quantile correlation and effective expression support of pooled expression ratio of reference genes for defining expression stability

Priyadarshi¹,

Das

Kumar

et al. 2017

Heliyon

Self Cite

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“…They observed 3-5 fold enhanced expression of the androgenic gland hormone (AGH) transcript in males, but there was no effect on vitellogenin expression in females. In the destalked animals, however, positive effect on the maturation indicator transcripts was seen in both the sexes, and surpassed the efficiency of the silencing treatment [26].…”

Section: Gene Editing Technologymentioning

confidence: 99%

“…Treerattrakool et al (2011) while working on P. monodon used the technique of RNA interference for inducement of maturation both in wild and captive shrimp and reported that shrimps injected with anti-GIH double stranded (ds) RNA showed advanced maturation [25]. Das et al (2015) reported silencing of gonad inhibiting hormone gene in the eyestalk neuroendocrine complex of the tiger shrimp P. monodon by using the technique of RNA interference. They observed 3-5 fold enhanced expression of the androgenic gland hormone (AGH) transcript in males, but there was no effect on vitellogenin expression in females.…”

Section: Gene Editing Technologymentioning

confidence: 99%

“…This may pave the way to develop an alternative and powerful technique in place of eyestalk ablation with similar impact. Efforts have been made to silence/knockout this gene using the technique of RNA interference by some workers but the success achieved is not as per the expectations and further research is needed in this area [25,26]. Treerattrakool et al (2011) while working on P. monodon used the technique of RNA interference for inducement of maturation both in wild and captive shrimp and reported that shrimps injected with anti-GIH double stranded (ds) RNA showed advanced maturation [25].…”

Section: Gene Editing Technologymentioning

confidence: 99%

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Gene editing (CRISPR-Cas) technology and fisheries sector

Ninawe¹,

Harke²

2017

Can J Biotech

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| P a g e AbstractConsidering the advantages of gene editing technologies, in recent years, emphasis has been given on three main techniques of gene editing i.e. zinc finger nucleases (ZFNs), transcription activator-like effector nucleases (TALENs), and the CRISPR/Cas9 RNA-guided endonuclease system. In all of these three technologies one thing is common i.e. the technology utilizes restriction enzymes to break down the double stranded DNA molecule at a targeted location with the help of another molecule of homologous binding protein or RNA, which is also called as a guide RNA molecule. This targeted breaking and repair of the DNA molecule as per our requirement is generally viewed as a great breakthrough in gene therapy methods. In fisheries sector, in order to promote aquaculture/mariculture activities, aquaculture industries are facing number of challenges, particularly in area of quality and demand of seed production, control of health and disease management, production of quality traits with phenotypically improved varieties, and strengthening of immune system. Several efforts are being made both at public and private sectors to develop scientific technologies to meet these challenges and substantial achievements have also been made. But still these challenges remained as major constraints in hampering the growth of this industry as per the demand and expectations. However, with the advent of now recently developed gene editing techniques, we may have to explore and evaluate the possibilities of applications of this technology as a long lasting solution in addressing at least some of the vital issues of the aquaculture industry particularly in those related to altering targeted gene structure in the species for positive impact.

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2017

Sexual Biology and Reproduction in Crustaceans

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Captive maturation studies in Penaeus monodon by GIH silencing using constitutively expressed long hairpin RNA

Cited by 21 publications

References 33 publications

Analysis of variance, normal quantile-quantile correlation and effective expression support of pooled expression ratio of reference genes for defining expression stability

Analysis of variance, normal quantile-quantile correlation and effective expression support of pooled expression ratio of reference genes for defining expression stability

Gene editing (CRISPR-Cas) technology and fisheries sector

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