Capsulorhexis: Pearls and pitfalls

Mohammadpour, Mehrdad; Erfanian, Reza; Karimi, Nasser

doi:10.1016/j.sjopt.2011.10.007

Cited by 29 publications

(16 citation statements)

References 73 publications

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“…During cataract surgery, a circular segment of the anterior lens capsule is cut open in a procedure called anterior capsulorhexis (Mohammadpour et al ., 2012). The lens fiber mass is then emulsified using a probe inserted through this opening and suctioned out in a procedure commonly known as phacoemulsification.…”

Section: Introductionmentioning

confidence: 99%

AGEs in human lens capsule promote the TGFβ2‐mediated EMT of lens epithelial cells: implications for age‐associated fibrosis

et al. 2016

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SummaryProteins in basement membrane (BM) are long‐lived and accumulate chemical modifications during aging; advanced glycation endproduct (AGE) formation is one such modification. The human lens capsule is a BM secreted by lens epithelial cells. In this study, we have investigated the effect of aging and cataracts on the AGE levels in the human lens capsule and determined their role in the epithelial‐to‐mesenchymal transition (EMT) of lens epithelial cells. EMT occurs during posterior capsule opacification (PCO), also known as secondary cataract formation. We found age‐dependent increases in several AGEs and significantly higher levels in cataractous lens capsules than in normal lens capsules measured by LC‐MS/MS. The TGFβ2‐mediated upregulation of the mRNA levels (by qPCR) of EMT‐associated proteins was significantly enhanced in cells cultured on AGE‐modified BM and human lens capsule compared with those on unmodified proteins. Such responses were also observed for TGFβ1. In the human capsular bag model of PCO, the AGE content of the capsule proteins was correlated with the synthesis of TGFβ2‐mediated α‐smooth muscle actin (αSMA). Taken together, our data imply that AGEs in the lens capsule promote the TGFβ2‐mediated fibrosis of lens epithelial cells during PCO and suggest that AGEs in BMs could have a broader role in aging and diabetes‐associated fibrosis.

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Section: Introductionmentioning

confidence: 99%

AGEs in human lens capsule promote the TGFβ2‐mediated EMT of lens epithelial cells: implications for age‐associated fibrosis

et al. 2016

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“…In conventional capsulorhexis, the capsular edge may tear away toward periphery due to centripetal zonular forces, which adversely affects the success of capsulorhexis. To eliminate these forces in such instances, several prevention and correction methods are recommended [25][26][27][28] . Because of the round structure of cerclage wire used in Group 2, the instrument did not have enough contact area with the lens surface and the capsule was torn towards the periphery mostly in the direction of 8 to 10 o'clock, most probably due to insufficient pressure against the capsular surface.…”

Section: Discussionmentioning

confidence: 99%

Yeni Bir El Aleti Kullanarak Bimanuel Kapsüloreksis: Koyun Gözünde Deneysel Çalışma

Karadayı¹,

Gültekin²,

Aytaç³

2018

Kafkas Univ Vet Fak Derg

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The purpose of this study was to design a cheap and easy-to-use hand tool that allows a better-centered capsulorhexis of consistent shape and size for inexperienced and experienced surgeons in cataract surgery. Forty-five eyes of dead adult sheep from a local slaughterhouse were grouped as; group 1, the continuous curvilinear capsulorhexis (CCC) group (n: 15); group 2, the cerclage wire group (n: 15); and group 3, the polylactic acid (PLA) hand tool group (n: 15). In group 1, one corneal incision was made. In groups 2 and 3, two incisions were made at 3:00 and 11:00 o'clock directions. Methylene blue was used for capsular staining. After injecting viscoelastic into the anterior chamber and the capsule was punctured, the devices were inserted at 3:00 o'clock. All capulorhexes were performed using the Utrata forceps following the internal contour of the devices. As a result of the study, capsulorhexes were successfully performed in 9 of 15 eyes in group 1; 2 of 15 eyes in group 2; and 12 of 15 eyes in group 3. Statistically, the correlation between the distributions of groups 1 and 2 (χ²=7.033, P=0.008); and for groups 1 and 3 (χ²=6.171, P=0.002) were found to be significant. This study showed that CCC was performed using the newly designed PLA hand tool more successfully than the classical method by the beginning surgeon.

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“…The rest of the lens is formed of terminally differentiated and elongated fiber cells, which are rich in proteins known as crystallins. During cataract surgery, a circular portion of the anterior capsule of the lens is excised (capsulorhexis), which allows surgeons to access the cataractous fiber material, emulsify, and suction it out of the lens, leaving behind a hollow bag of lens capsule . An intraocular lens (IOL) is implanted in the capsular bag.…”

Section: Introductionmentioning

confidence: 99%

The role of HIF‐1α in the TGF‐β2‐mediated epithelial‐to‐mesenchymal transition of human lens epithelial cells

Nahomi

Nagaraj

2018

J of Cellular Biochemistry

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Human lens epithelial cells (HLE) undergo mesenchymal transition and become fibrotic during posterior capsule opacification (PCO), which is a frequent complication after cataract surgery. TGF-β2 has been implicated in this fibrosis. Previous studies have focused on the role of hypoxia-inducible factor-1α (HIF-1α) in fibrotic diseases, but the role of HIF-1α in the TGF-β2-mediated fibrosis in HLE is not known. TGF-β2 treatment (10 ng/mL, 48 h) increased the HIF-1α levels along with the EMT markers in cultured human lens epithelial cells (FHL124 cells). The increase in HIF-1α corresponded to an increase in VEGF-A in the culture medium. However, exogenous addition of VEGF-A (up to 10 ng/mL) did not alter the EMT marker levels in HLE. Addition of a prolyl hydroxylase inhibitor, dimethyloxalylglycine (DMOG, up to 10 µM), enhanced the levels of HIF-1α, and secreted VEGF-A but did not alter the EMT marker levels. However, treatment of cells with a HIF-1α translational inhibitor, KC7F2, significantly reduced the TGF-β2-mediated EMT response. This was accompanied by a reduction in the ERK phosphorylation and nuclear translocation of Snail and Slug. Together, these data suggest that HIF-1α is important for the TGF-β2-mediated EMT of human lens epithelial cells.

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Capsulorhexis: Pearls and pitfalls

Cited by 29 publications

References 73 publications

AGEs in human lens capsule promote the TGFβ2‐mediated EMT of lens epithelial cells: implications for age‐associated fibrosis

AGEs in human lens capsule promote the TGFβ2‐mediated EMT of lens epithelial cells: implications for age‐associated fibrosis

Yeni Bir El Aleti Kullanarak Bimanuel Kapsüloreksis: Koyun Gözünde Deneysel Çalışma

The role of HIF‐1α in the TGF‐β2‐mediated epithelial‐to‐mesenchymal transition of human lens epithelial cells

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