Capsular Polysaccharides Produced by the Bacterial Pathogen Burkholderia pseudomallei

Reckseidler-Zenteno, Shauna L.

doi:10.5772/50116

Cited by 8 publications

(4 citation statements)

References 75 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…B. pseudomallei encodes a highly immunogenic capsular polysaccharide (CPS-I), and a type II O-antigenic polysaccharide (O-PS) moiety of lipopolysaccharide (LPS), both of which are required for serum resistance and virulence (61, 62). We have previously documented a 1-bp loss-of-function indel within the CPS-I gene, wcbR , which occurred prior to retrieval of the first isolate and which persisted throughout the duration of the chronic-carriage infection (14).…”

Section: Resultsmentioning

confidence: 99%

See 1 more Smart Citation

Within-Host Evolution of Burkholderia pseudomallei during Chronic Infection of Seven Australasian Cystic Fibrosis Patients

Viberg

Sarovich

Kidd

et al. 2017

mBio

130

View full text Add to dashboard Cite

Cystic fibrosis (CF) is a genetic disorder characterized by progressive lung function decline. CF patients are at an increased risk of respiratory infections, including those by the environmental bacterium Burkholderia pseudomallei, the causative agent of melioidosis. Here, we compared the genomes of B. pseudomallei isolates collected between ~4 and 55 months apart from seven chronically infected CF patients. Overall, the B. pseudomallei strains showed evolutionary patterns similar to those of other chronic infections, including emergence of antibiotic resistance, genome reduction, and deleterious mutations in genes involved in virulence, metabolism, environmental survival, and cell wall components. We documented the first reported B. pseudomallei hypermutators, which were likely caused by defective MutS. Further, our study identified both known and novel molecular mechanisms conferring resistance to three of the five clinically important antibiotics for melioidosis treatment. Our report highlights the exquisite adaptability of microorganisms to long-term persistence in their environment and the ongoing challenges of antibiotic treatment in eradicating pathogens in the CF lung. Convergent evolution with other CF pathogens hints at a degree of predictability in bacterial evolution in the CF lung and potential targeted eradication of chronic CF infections in the future.

show abstract

Section: Resultsmentioning

confidence: 99%

“…Only CF11 exhibited a mutation in CPS-I, with the latter isolate encoding a 1-bp deletion in wcbA ( BPSL2809 ). WcbA, a capsular export protein, is critical for B. pseudomallei virulence (62). This mutation causes a frameshift at residue A434 that truncates this protein by 70 amino acids and likely abrogates CPS-I production in this strain.…”

Section: Resultsmentioning

confidence: 99%

Within-Host Evolution of Burkholderia pseudomallei during Chronic Infection of Seven Australasian Cystic Fibrosis Patients

Viberg

Sarovich

Kidd

et al. 2017

mBio

130

View full text Add to dashboard Cite

show abstract

“…In sea ice, bacteria may acquire similar protection by producing capsular polysaccharides, as genes involved in transporting and exporting capsular polysaccharide components were more abundant there (Supplementary Table 4). These EPS remain intimately associated with the cell surface, where they can act as an antibiotic (and possible viral) shield, while also aiding in surface attachment and biofilm formation (Reckseidler-Zenteno, 2012) or cryoprotection (Carillo et al, 2015).…”

Section: Elaborate Competitive Strategies At High Cell Densities In Subzero Brinesmentioning

confidence: 99%

Divergent Genomic Adaptations in the Microbiomes of Arctic Subzero Sea-Ice and Cryopeg Brines

2021

View full text Add to dashboard Cite

Subzero hypersaline brines are liquid microbial habitats within otherwise frozen environments, where concentrated dissolved salts prevent freezing. Such extreme conditions presumably require unique microbial adaptations, and possibly altered ecologies, but specific strategies remain largely unknown. Here we examined prokaryotic taxonomic and functional diversity in two seawater-derived subzero hypersaline brines: first-year sea ice, subject to seasonally fluctuating conditions; and ancient cryopeg, under relatively stable conditions geophysically isolated in permafrost. Overall, both taxonomic composition and functional potential were starkly different. Taxonomically, sea-ice brine communities (∼105 cells mL–1) had greater richness, more diversity and were dominated by bacterial genera, including Polaribacter, Paraglaciecola, Colwellia, and Glaciecola, whereas the more densely inhabited cryopeg brines (∼108 cells mL–1) lacked these genera and instead were dominated by Marinobacter. Functionally, however, sea ice encoded fewer accessory traits and lower average genomic copy numbers for shared traits, though DNA replication and repair were elevated; in contrast, microbes in cryopeg brines had greater genetic versatility with elevated abundances of accessory traits involved in sensing, responding to environmental cues, transport, mobile elements (transposases and plasmids), toxin-antitoxin systems, and type VI secretion systems. Together these genomic features suggest adaptations and capabilities of sea-ice communities manifesting at the community level through seasonal ecological succession, whereas the denser cryopeg communities appear adapted to intense bacterial competition, leaving fewer genera to dominate with brine-specific adaptations and social interactions that sacrifice some members for the benefit of others. Such cryopeg genomic traits provide insight into how long-term environmental stability may enable life to survive extreme conditions.

show abstract

“…[1] Thecapsular polysaccharides protect the microorganism from adverse environmental conditions,prevent the activation of the host immune system and improve adhesion to invade the host. [2][3][4] Capsular polysaccharides of pathogens have been used to produce vaccines that are effective in adults, [5,6] but result ineffective in children below 2years of age: [7][8][9] being T-cell-independent antigens,t hey do not induce B-cell memory and do not stimulate the production of immunoglobulin G( IgG). However,aT-cell-dependent response (thus inducing B-cell memory) can be obtained by chemical coupling of polysaccharides to immunogenic proteins.P olysaccharide-protein conjugates are commonly obtained by covalent linkage of reactive precursors to the e-amino groups of surface-exposed lysine residues or other reactive amino acids.…”

mentioning

confidence: 99%

Characterization of the Conjugation Pattern in Large Polysaccharide–Protein Conjugates by NMR Spectroscopy

et al. 2017

View full text Add to dashboard Cite

Carbohydrate-based vaccines are among the safest and most effective vaccines and represent potent tools for prevention of life-threatening bacterial infectious diseases,like meningitis and pneumonia. The chemical conjugation of aw eak antigen to protein as as ource of T-cell epitopes generates aglycoconjugate vaccine that results more immunogenic. Several methods have been used so far to characterize the resulting polysaccharide-protein conjugates.H owever, ar educed number of methodologies has been proposed for measuring the degree of saccharideconjugation at the possible protein sites.Here we show that detailed information on large proteins conjugated with large polysaccharides can be achieved by ac ombination of solution and solid-state NMR spectroscopy. As at est case,alarge protein assembly, l-asparaginase II, has been conjugated with Neisseria meningitidis serogroup Cc apsular polysaccharide and the pattern and degree of conjugation were determined.

show abstract

Capsular Polysaccharides Produced by the Bacterial Pathogen Burkholderia pseudomallei

Cited by 8 publications

References 75 publications

Within-Host Evolution of Burkholderia pseudomallei during Chronic Infection of Seven Australasian Cystic Fibrosis Patients

Within-Host Evolution of Burkholderia pseudomallei during Chronic Infection of Seven Australasian Cystic Fibrosis Patients

Divergent Genomic Adaptations in the Microbiomes of Arctic Subzero Sea-Ice and Cryopeg Brines

Characterization of the Conjugation Pattern in Large Polysaccharide–Protein Conjugates by NMR Spectroscopy

Contact Info

Product

Resources

About