Capsular Polysaccharides of Lactobacillus spp.: Theoretical and Practical Aspects of Simple Visualization Methods

Oleksy, Magdalena; Klewicka, Elżbieta

doi:10.1007/s12602-017-9295-5

Cited by 8 publications

(5 citation statements)

References 44 publications

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“…The value of each protein band was estimated using the TotalLab Analysis Software (ver.1.0.1) and the UVP GelDoc-Ite gel documentation system. The integrity of the LAG capsule was assessed by light microscopy following staining with crystal violet/copper sulfate (Anthony's stain) consisting of crystal violet as the primary stain and 20% copper sulfate solution as a decolorizing solution and counter stain [48]. Finally, the LAG surface negativity was examined using nigrosine staining, performed by mixing a small drop of nigrosine solution (10% w/v) with a small drop of bacterial or ghost suspension near the end of a glass slide.…”

Section: Characterizationmentioning

confidence: 99%

Prodigiosin-Functionalized Probiotic Ghosts as a Bioinspired Combination Against Colorectal Cancer Cells

Saleh

Mahmoud

Eltaher

et al. 2022

Probiotics & Antimicro. Prot.

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Lactobacillus acidophilus ghosts (LAGs) with the unique safety of a probiotic, inherent tropism for colon cells, and multiple bioactivities offer promise as drug carriers for colon targeting. Our objective was to evaluate LAGs functionalized with prodigiosin (PG), apoptotic secondary bacterial metabolite, as a bioinspired formulation against colorectal cancer (CRC). LAGs were prepared by a chemical method and highly purified by density gradient centrifugation. LAGs were characterized by microscopic and staining techniques as relatively small-sized uniform vesicles (≈1.6 µm), nearly devoid of cytoplasmic and genetic materials and having a negatively charged intact envelope. PG was highly bound to LAGs envelope, generating a physiologically stable bioactive entity (PG-LAGs), as verified by multiple microscopic techniques and lack of PG release under physiological conditions. PG-LAGs were active against HCT116 CRC cells at both the cellular and molecular levels. Cell viability data highlighted the cytotoxicity of PG and LAGs and LAGs-induced enhancement of PG selectivity for HCT116 cells, anticipating dose reduction for PG and LAGs. Molecularly, expression of the apoptotic caspase 3 and P53 biomarkers in HCT116 intracellular proteins was significantly upregulated while that of the anti-apoptotic Bcl-2 (B-cell lymphoma 2) was downregulated by PG-LAGs relative to PG and 5-fluorouracil. PG-LAGs provide a novel bacteria-based combination for anticancer biomedicine. Graphical abstract

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Section: Characterizationmentioning

confidence: 99%

Prodigiosin-Functionalized Probiotic Ghosts as a Bioinspired Combination Against Colorectal Cancer Cells

Saleh

Mahmoud

Eltaher

et al. 2022

Probiotics & Antimicro. Prot.

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“…Figure 2 shows a decolorized halo around the bacteria corresponding to the capsule produced by L. rhamnosus P14. Several studies were based on this observation for screening EPS‐producers, indicating the presence of bacterial capsules around Streptococcus thermophilus , Lactobacillus acidophilus , Lactobacillus casei , and others (Oleksy & Klewicka, 2017; Pachekrepapol et al., 2017). Therefore, the determination of colony character and polysaccharide type helps us to choose the correct application for the bacteria and its EPS.…”

Section: Resultsmentioning

confidence: 93%

Investigation on exopolysaccharide production by Lacticaseibacillus rhamnosus P14 isolated from Moroccan raw cow's milk

Akhtach

Tabia

Bricha

et al. 2021

Journal of Food Science

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Twenty‐four strains were isolated from 50 samples of raw cow's milk originated from different regions of Morocco. After different screening methods, one strain was selected as the highest exopolysaccharide (EPS)‐producing isolate and was identified by 16S rDNA sequencing as Lacticaseibacillus rhamnosus P14. Moreover, the EPS‐producing ability, bacterial growth, and pH of the medium were monitored. The optimization of culture conditions indicated that the high yield of EPS was 685.14 mg/L obtained at 42°C, with lactose as a carbon source. The characterization study showed that the purified EPS consisted of one main fraction that contained 97.67% of carbohydrates. Furthermore, the EPS was identified as a homogeneous polysaccharide, mainly composed of glucose. These results demonstrated the high EPS production ability of the selected L. rhamnosus P14, representing a promising candidate to improve the textural and sensory properties of fermented food.

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“…Additionally, the regular background can interfere with the image of the capsules. If the film is pressed too thin, the capsules may become flattened, distorted, and possibly disintegrated [44].…”

Section: Discussionmentioning

confidence: 99%

Efficient Screening and Enhanced Exopolysaccharide Production by Functional Lactic Acid Bacteria (LAB) in Lactose Supplemented Media

I. E. Pintado,

C. R. Barbosa,

Pintado

et al. 2024

Applied Microbiology: Theory ＆ Technology

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Exopolysaccharides (EPS) produced by lactic acid bacteria (LAB) can be considered as natural biological thickeners that have attracted considerable attention in the food industry. This study aimed to evaluate and select potentially EPS-producing strains LAB and to assess the influence of carbon source and aeration on EPS production. Nine LAB strains were assessed as potential EPS producers, and Rahnella aquatilis ATCC 55046 was employed as the positive control strain for EPS. The compaction test and the observation of viscous colonies in a solid medium did not yield sufficient evidence for the presence of EPS. The assessment of capsules through staining provided evidence of EPS presence only for Rahnella aquatilis ATCC 55046. The EPS yield was subsequently assessed in De-Man Rogosa and Sharpe (MRS) broth medium supplemented with 2% (w/w) fructose (MRS-f) or lactose (MRS-l), as well as in whey (Whey) and whey supplemented with 2% (w/w) lactose (Whey-l). The EPS production in the various culture media under study ranged from 194 to 1,187 mg of EPS/g of polymer dry mass (PDM). These results suggest that the culture medium and carbon sources had an impact on the EPS production of the different strains. Bifidobacterium animalisBb12 achieved the highest EPS production in MRS-f. In the case of MRS-l, the control strain recorded the highest EPS value, along with Lactobacillus acidophilus LAC-1. Regarding Whey, Lentilactobacillus Kefir NCFB 2753 exhibited the highest EPS production, while in Whey-l, Lacticaseibacillus paracasei LCS-1 emerged as the top performer in terms of EPS production. This suggests that certain strains exhibit potential for use in the production of novel fermented EPS products, whether dairy or non-dairy.

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Capsular Polysaccharides of Lactobacillus spp.: Theoretical and Practical Aspects of Simple Visualization Methods

Cited by 8 publications

References 44 publications

Prodigiosin-Functionalized Probiotic Ghosts as a Bioinspired Combination Against Colorectal Cancer Cells

Prodigiosin-Functionalized Probiotic Ghosts as a Bioinspired Combination Against Colorectal Cancer Cells

Investigation on exopolysaccharide production by Lacticaseibacillus rhamnosus P14 isolated from Moroccan raw cow's milk

Efficient Screening and Enhanced Exopolysaccharide Production by Functional Lactic Acid Bacteria (LAB) in Lactose Supplemented Media

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