Capsaicin potently blocks <i>Salmonella typhimurium</i> invasion of Vero cells

Ayariga, Joseph A.; Abugri, Daniel A.; Amrutha, Balagopal; Villafañe, Robert

doi:10.1101/2022.01.02.474733

Cited by 7 publications

(1 citation statement)

References 46 publications

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“…As this facultative anaerobe continues to cause serious public health problems, the need to investigate Salmonella has received much scientific scrutiny [61][62][63][64]. In this study, we demonstrated the antibacterial activity of CBD against two relevant pathogenic bacteria, S. typhimurium and S. newington.…”

Section: Discussionmentioning

confidence: 85%

Cannabis sativa CBD Extract Shows Promising Antibacterial Activity against Salmonella typhimurium and S. newington

et al. 2022

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Products derived from Cannabis sativa L. have gained increased interest and popularity. As these products become common amongst the public, the health and potential therapeutic values associated with hemp have become a premier focus of research. While the psychoactive and medicinal properties of Cannabis products have been extensively highlighted in the literature, the antibacterial properties of cannabidiol (CBD) have not been explored in depth. This research serves to examine the antibacterial potential of CBD against Salmonella newington and S. typhimurium. In this study, we observed bacterial response to CBD exposure through biological assays, bacterial kinetics, and fluorescence microscopy. Additionally, comparative studies between CBD and ampicillin were conducted against S. typhimurium and S. newington to determine comparative efficacy. Furthermore, we observed potential resistance development of our Salmonella spp. against CBD treatment.

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Section: Discussionmentioning

confidence: 85%

Cannabis sativa CBD Extract Shows Promising Antibacterial Activity against Salmonella typhimurium and S. newington

et al. 2022

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Proteolytic Analysis of Epsilon 34 Phage Tailspike protein indicates Partial Sensitivity to Proteinase K

Ayariga

Villafañe

2022

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Purified bacteriophage Ɛ34 tailspike protein (Ɛ34 TSP) can bind to Salmonella newington (S. newington) via the binding site of the protein, which is the O antigens of the LPS of the bacterium. We demonstrated elsewhere that purified Ɛ34 TSP possessed bacteria lytic property on S. newington. The Ɛ34 TSP has been shown via computational prediction to consist of parallel β-helices like that of P22 TSP. These protein moieties are among the simplest repetitive structural elements in proteins. There exist extensive research on the folding behavior of β-helix proteins, which also provides insight on how amyloid fibrils are generated since these proteins consist of similar parallel β-helix motifs. One of the most significantly studied system for investigating protein folding is the from the Salmonella bacteriophage P22. The major component of this protein is a right-handed parallel β-helix with 13 rungs. Initial in silico analysis of the Ɛ34 phage TSP indicates similar structural similarity to the P22 TSP. Our previous studies indicated that despite the similarities of the two proteins, P22 TSP shows higher resistance to proteases (e.g. trypsin) and heat compared to Ɛ34 TSP. In this study we further proof that Ɛ34 TSP is partially sensitive to proteinase K, whereas P22 TSP is completely resistant to this protein. Detailed analysis indicates that specific structural motifs of Ɛ34 TSP is insensitive to the protease, whereas other regions of the protein showed susceptibility to it.

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Eco-Friendly Sol–Gel Coatings as Microfouling Barrier for Marine Applications

Diaz Jalaff,

Ortega Cancino,

Altavilla

et al. 2023

Coatings

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Biofouling poses a significant challenge to the marine industry, resulting from the undesirable accumulation of various living organisms, including microorganisms, plants, shellfish, and others, on different surfaces submerged in seawater. The primary approach to address this issue involves the application of antifouling coatings, often incorporating active agents whose environmental impact has raised considerable concerns. In this article, we compare the effects of two natural and environmentally friendly antimicrobial compounds, capsaicin (CP) and Cinnamomum cassia oil (CO), when incorporated into hybrid sol–gel coatings. We tested formulations containing 4% and 8% by weight of each biocide for coating nylon substrates through immersion. This process yielded colorless, uniform surfaces with minimal defects and strong adhesion. We evaluated the antibacterial properties of the pure organic biocides against five bacterial strains commonly found in Chilean microfouling. Both compounds exhibited similar behavior against Gram-positive bacteria, but CP showed 2–5 times greater efficacy than CO against Gram-negative strains. Subsequently, we selected a Gram-negative strain for antibacterial tests on sol–gel/biocide samples. The results indicated that coatings with CP incorporation outperformed those with CO, achieving an efficiency of 99.9%. Lastly, leaching tests demonstrated that coatings with lower biocide concentrations (4%) exhibited a steady and gradual release of organic molecules, with sol–gel/CP 4% emerging as the most promising microfouling barrier coating for marine applications.

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Capsaicin potently blocks Salmonella typhimurium invasion of Vero cells

Cited by 7 publications

References 46 publications

Cannabis sativa CBD Extract Shows Promising Antibacterial Activity against Salmonella typhimurium and S. newington

Cannabis sativa CBD Extract Shows Promising Antibacterial Activity against Salmonella typhimurium and S. newington

Proteolytic Analysis of Epsilon 34 Phage Tailspike protein indicates Partial Sensitivity to Proteinase K

Eco-Friendly Sol–Gel Coatings as Microfouling Barrier for Marine Applications

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