Capsaicin blocks Ca2+ channels in isolated rat trigeminal and hippocampal neurones

Kopanitsa, M. V.; Panchenko, V. A.; Magura, E. I.; Lishko, Polina V.; Krishtal, O. A.

doi:10.1097/00001756-199511270-00016

Cited by 24 publications

(19 citation statements)

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“…Based on our calcium imaging studies, we speculate that Theobroma cacao repression of trigeminal neuron activity involves blockage of the normal physiological increase in intracellular calcium levels in response to KCl and capsaicin. It is well established that the cellular effects of KCl and capsaicin can be mediated by activation of calcium channels (Bell, 1993;Kopanitsa et al, 1995;Brosenitsch et al, 1998;Mohri et al, 2003). Our finding is in agreement with a recent study that reported an inhibitory effect of the flavonols, a class of plant polyphenols, on stimulated interleukin secretion which involved suppression of intracellular calcium levels in cultured mast cells (Kempuraj et al, 2005).…”

Section: Discussionsupporting

confidence: 93%

Repression of calcitonin gene-related peptide expression in trigeminal neurons by a Theobroma cacao extract

Abbey

Patil

Vause

et al. 2008

Journal of Ethnopharmacology

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Ethnopharmacological relevance-Cocoa bean preparations were first used by the ancient Maya and Aztec civilizations of South America to treat a variety of medical ailments involving the cardiovascular, gastrointestinal, and nervous systems. Diets rich in foods containing abundant polyphenols, as found in cocoa, underlie the protective effects reported in chronic inflammatory diseases. Release of calcitonin gene-related peptide (CGRP) from trigeminal nerves promotes inflammation in peripheral tissues and nociception.Aim of the study-To determine whether a methanol extract of Theobroma cacao L. (Sterculiaceae) beans enriched for polyphenols could inhibit CGRP expression, both an in vitro and an in vivo approach was taken.Results-Treatment of rat trigeminal ganglia cultures with depolarizing stimuli caused a significant increase in CGRP release that was repressed by pretreatment with Theobroma cacao extract. Pretreatment with Theobroma cacao was also shown to block the KCl-and capsaicin-stimulated increases in intracellular calcium. Next, the effects of Theobroma cacao on CGRP levels were determined using an in vivo model of temporomandibular joint (TMJ) inflammation. Capsaicin injection into the TMJ capsule caused an ipsilateral decrease in CGRP levels. Theobroma cacao extract injected into the TMJ capsule 24 h prior to capsaicin treatment repressed the stimulatory effects of capsaicin.Conclusions-Our results demonstrate that Theobroma cacao extract can repress stimulated CGRP release by a mechanism that likely involves blockage of calcium channel activity. Furthermore, our findings suggest that the beneficial effects of diets rich in cocoa may include suppression of sensory trigeminal nerve activation.

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Section: Discussionsupporting

confidence: 93%

Repression of calcitonin gene-related peptide expression in trigeminal neurons by a Theobroma cacao extract

Abbey

Patil

Vause

et al. 2008

Journal of Ethnopharmacology

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“…The latter possibility is indirectly supported by the observation that the release of GABA from synaptosomes isolated from the rat hippocampus is suppressed by capsaicin [8]. The earlier described blocking of voltage-activated calcium channels in hippocampal neurons by capsaicin [9] is also in line with this hypothesis. The possibility that capsaicin in our experiments preferentially affects neuronal firing in a subpopulation of GABA-ergic interneurons evoking IPSCs of a larger amplitude is an alternative or an additional way to explain the discrepancy between our results and those obtained earlier [5].…”

Section: Resultssupporting

confidence: 53%

An unexpected effect of capsaicin on spontaneous GABA-ergic IPSCS in hippocampal cell cultures

2006

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Vanilloid receptors 1 (VRs1) expressed in a subpopulation of sensory neurons and responsible for processing of chemical and thermal noxious stimuli were also shown to be expressed in several cerebral structures and to be involved in the regulation of glutamatergic synaptic transmission. In this study, we started to investigate the possibility that VRs1 are also involved in the regulation of GABA-ergic synaptic transmission. For this purpose, the effect of a VR1 agonist, capsaicin, on spontaneous GABA-ergic inhibitory postsynaptic currents (IPSCs) was studied in hippocampal cell cultures using a patch-clamp technique. It was found that capsaicin (10 µM) decreased both the frequency and amplitude of spontaneous IPSCs. This finding suggests the involvement of VRs1 in the regulation of neuronal firing in some GABA-ergic interneurons and in the modulation of the efficacy of GABA-ergic synaptic transmission. However, considering the direction of the effect (a decrease in the IPSC frequency) and lack of its desensitization, the involvement of other receptor(s) also cannot currently be ruled out.

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“…The presence of VR1-expressing neurons in the limbic system is not unexpected either. Capsaicin was shown to block Ca 2ϩ channels in isolated trigeminal and hippocampal neurons with similar potencies (25). Other groups also have detected mRNA for VR1 in the hippocampus by RT-PCR (15).…”

Section: Discussionmentioning

confidence: 99%

Distribution of mRNA for vanilloid receptor subtype 1 (VR1), and VR1-like immunoreactivity, in the central nervous system of the rat and human

Mezey¹

2000

Proceedings of the National Academy of Sciences

384

355

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The cloned vanilloid receptor VR1 has attracted recent attention as a molecular integrator of painful stimuli on primary sensory neurons. The existence of vanilloid-sensitive neurons in the brain is, however, controversial. In this study, we have used an antibody and a complementary RNA probe to explore the distribution of neurons that express VR1 in rat and in certain areas of human brain. In the rat, we observed VR1-expressing neurons throughout the whole neuroaxis, including all cortical areas (in layers 3 and 5), several members of the limbic system (e.g., hippocampus, central amygdala, and both medial and lateral habenula), striatum, hypothalamus, centromedian and paraventricular thalamic nuclei, substantia nigra, reticular formation, locus coeruleus, cerebellum, and inferior olive. VR1-immunopositive cells also were found in the third and fifth layers of human parietal cortex. Reverse transcription-PCR performed with rat VR1-specific primers verified the expression of VR1 mRNA in cortex, hippocampus, and hypothalamus. In the central nervous system, neonatal capsaicin treatment depleted VR1 mRNA from the spinal nucleus of the trigeminal nerve, but not from other areas such as the inferior olive. The finding that VR1 is expressed not only in primary sensory neurons but also in several brain nuclei is of great importance in that it places VRs in a much broader perspective than pain perception. VRs in the brain (and putative endogenous vanilloids) may be involved in the control of emotions, learning, and satiety, just to name a few exciting possibilities.

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Capsaicin blocks Ca2+ channels in isolated rat trigeminal and hippocampal neurones

Cited by 24 publications

References 0 publications

Repression of calcitonin gene-related peptide expression in trigeminal neurons by a Theobroma cacao extract

Repression of calcitonin gene-related peptide expression in trigeminal neurons by a Theobroma cacao extract

An unexpected effect of capsaicin on spontaneous GABA-ergic IPSCS in hippocampal cell cultures

Distribution of mRNA for vanilloid receptor subtype 1 (VR1), and VR1-like immunoreactivity, in the central nervous system of the rat and human

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