CAPS1 is involved in hippocampal synaptic plasticity and hippocampus-associated learning

Abstract

Calcium-dependent activator protein for secretion 1 (CAPS1) is a key molecule in vesicular exocytosis, probably in the priming step. However, CAPS1’s role in synaptic plasticity and brain function is elusive. Herein, we showed that synaptic plasticity and learning behavior were impaired in forebrain and/or hippocampus-specific Caps1 conditional knockout (cKO) mice by means of molecular, physiological, and behavioral analyses. Neonatal Caps1 cKO mice showed a decrease in the number of docked vesicles in the hippocampal CA3 region, with no detectable changes in the distribution of other major exocytosis-related molecules. Additionally, long-term potentiation (LTP) was partially and severely impaired in the CA1 and CA3 regions, respectively. CA1 LTP was reinforced by repeated high-frequency stimuli, whereas CA3 LTP was completely abolished. Accordingly, hippocampus-associated learning was severely impaired in adeno-associated virus (AAV) infection-mediated postnatal Caps1 cKO mice. Collectively, our findings suggest that CAPS1 is a key protein involved in the cellular mechanisms underlying hippocampal synaptic release and plasticity, which is crucial for hippocampus-associated learning.

Ca2+‐dependent activator protein for secretion 1 promotes spontaneous recovery in ischemic stroke by regulating BDNF secretion

“…In addition, CAPS1 can regulate the secretion and release of monoamine neurotransmitters and neuropeptides in synaptic vesicles of neuroendocrine cells. In vivo study, using CAPS1 knockout mice, it has shown that CAPS1 is an important protein in regulating the release of synaptic vesicles (Jockusch et al, 2007). Using the BDNF-GFP, study has confirmed that CAPS1 can affect BDNF secretion by regulating the secretion process of synaptic vesicles in hippocampal neurons (Eckenstaler et al, 2016).…”

“…However, the role of CAPS1 in brain function, especially in central nervous system‐related disease, is poorly understood. Recent study has shown that CAPS1 knockout mouse showed impairments in hippocampal long‐term potentiation (LTP), indicating the association of CAPS1 with learning ability (Ishii et al, 2021). We demonstrated for the first time that the expression of CAPS1 increased in the hippocampus after ischemic stroke.…”

Mir155hg Accelerates Hippocampal Neuron Injury in Convulsive Status Epilepticus by Inhibiting Microglial Phagocytosis

“…The Golgi staining was performed as previous study described [24].The isolated hippocampal tissue was xed with agar gel on the ice surface. Place the hippocampal tissue in a vibration section (vertical position, 380µM/piece).…”