CaPrx, a Coffea arabica gene encoding a putative class III peroxidase induced by root-knot nematode infection

Severino, Fabio; Brandalise, Marcos; Costa, Carolina S.; Wilcken, Sílvia Renata Siciliano; Maluf, Mirian Perez; Gonçalves, Wallace; Maia, Ivan de Godoy

doi:10.1016/j.plantsci.2012.04.012

Cited by 20 publications

(9 citation statements)

References 38 publications

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“…Thus, a strong burst in extracellular ROS production by host cell NADPH oxidases and peroxidases accompanies root knot nematode invasion at 12 to 24 h post invasion before declining to lower levels during giant cell formation (Melillo et al, 2006, 2011; Das et al, 2008). During the latter period, peroxidases are expressed in giant cells (Severino et al, 2012) that could regulate intracellular ROS levels to induce cell wall biosynthesis linked with ingrowth wall formation (Andriunas et al, 2012; Xia et al, 2012). Intracellularly-produced ROS in nodule founder cells (Lee et al, 2005) is essential for initiating nodule primordia in developing legume- Rhizobium symbioses (D'Haeze et al, 2003).…”

Section: Does An Auxin-ethylene-ros Signaling Cascade Regulate Phloemmentioning

confidence: 99%

Intersection of transfer cells with phloem biology—broad evolutionary trends, function, and induction

Andriunas¹,

Zhang²,

Xia³

et al. 2013

Front. Plant Sci.

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Transfer cells (TCs) are ubiquitous throughout the plant kingdom. Their unique ingrowth wall labyrinths, supporting a plasma membrane enriched in transporter proteins, provides these cells with an enhanced membrane transport capacity for resources. In certain plant species, TCs have been shown to function to facilitate phloem loading and/or unloading at cellular sites of intense resource exchange between symplasmic/apoplasmic compartments. Within the phloem, the key cellular locations of TCs are leaf minor veins of collection phloem and stem nodes of transport phloem. In these locations, companion and phloem parenchyma cells trans-differentiate to a TC morphology consistent with facilitating loading and re-distribution of resources, respectively. At a species level, occurrence of TCs is significantly higher in transport than in collection phloem. TCs are absent from release phloem, but occur within post-sieve element unloading pathways and particularly at interfaces between generations of developing Angiosperm seeds. Experimental accessibility of seed TCs has provided opportunities to investigate their inductive signaling, regulation of ingrowth wall formation and membrane transport function. This review uses this information base to explore current knowledge of phloem transport function and inductive signaling for phloem-associated TCs. The functional role of collection phloem and seed TCs is supported by definitive evidence, but no such information is available for stem node TCs that present an almost intractable experimental challenge. There is an emerging understanding of inductive signals and signaling pathways responsible for initiating trans-differentiation to a TC morphology in developing seeds. However, scant information is available to comment on a potential role for inductive signals (auxin, ethylene and reactive oxygen species) that induce seed TCs, in regulating induction of phloem-associated TCs. Biotic phloem invaders have been used as a model to speculate on involvement of these signals.

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Section: Does An Auxin-ethylene-ros Signaling Cascade Regulate Phloemmentioning

confidence: 99%

Intersection of transfer cells with phloem biology—broad evolutionary trends, function, and induction

Andriunas¹,

Zhang²,

Xia³

et al. 2013

Front. Plant Sci.

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“…New frontiers for second- and third-generation transgenic plants involve tissue-specific expression driven by specific promoters (Christou et al 2006 ). In a transgenic tobacco root assay, the promoter of a putative peroxidase-encoding gene from C. arabica (CaPrx) driving β-glucuronidase (GUS) expression was active in galls and was induced by root-knot nematode infection after 16 h (Severino et al 2012 ). Recently, promoter regions from an nsLTP (non-specific lipid-transfer protein) type II gene that is specifically expressed in coffee fruits were reported to promote grain-specific expression in transgenic tobacco plants when driving GUS expression, as observed by histochemical and fluorometric GUS assays (Cotta et al 2014 ).…”

Section: Discussionmentioning

confidence: 99%

Seed-Specific Stable Expression of the α-AI1 Inhibitor in Coffee Grains and the In Vivo Implications for the Development of the Coffee Berry Borer

Albuquerque

Bezerra

Romero

et al. 2015

Tropical Plant Biol.

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Genetic transformation of coffee (Coffea spp.), the second most traded commodity worldwide, is an alternative approach to introducing features that cannot be introgressed by traditional crossings. The transgenic stability, heritability and quantitative and spatial expression patterns of the seed-specific promoter phytohemagglutinin (PHA-L) from Phaseolus vulgaris were characterized in genetically modified C. arabica expressing the α-amylase inhibitor-1 (α-AI1) gene. The α-AI1 inhibitor shows considerable activity toward digestive enzymes of the coffee berry borer (CBB) Hypothenemus hampei. This insect pest expends its life cycle almost entirely in coffee berries. Transgene containment in the fruit is important to meeting food and environmental safety requirements for releasing genetically modified (GM) crops. PCR analysis of T2 coffee plants showed a Mendelian single-copy segregation pattern. Ectopic transgene expression was only detected in coffee grains, as demonstrated by reverse transcription-PCR analysis of different plant tissues. An intense immunocytochemical signal associated with α-AI1 protein expression was localized to endospermic cells. In addition, a delay in the larval development of CBB was observed after challenging transgenic coffee seeds with the insect. These results indicate that the PHA-L promoter might be a useful tool in coffee for the seed-specific expression of genes related to coffee bean productivity, quality and pest protection. The biotechnological applicability of the α-AI1 gene for controlling CBB is also discussed. This work is the first report showing a seed-specific transgene expression in coffee plants.Electronic supplementary materialThe online version of this article (doi:10.1007/s12042-015-9153-0) contains supplementary material, which is available to authorized users.

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“…In coffee, most studies evaluating organ/tissue-specific promoters have focused on seed-and fruit-specific genes (Marraccini et al 1999;Cotta et al 2014). Other examples include the promoter regions of genes involved in stress and defense responses (Brandalise et al 2009;Severino et al 2012;Petitot et al 2013;Nobres et al 2016;Alves et al 2017), in light-regulated carbon fixation (Marraccini et al 2003) and in caffeine biosynthesis (Satyanarayana et al 2005). In practice, however, the 35S promoter is still the preferred choice for the construction of expression cassettes employed in coffee biotechnology (Mishra and Slater 2012).…”

Section: Electronic Supplementary Materialsmentioning

confidence: 99%

Identification of a seed maturation protein gene from Coffea arabica (CaSMP) and analysis of its promoter activity in tomato

Quintero

Pinto

Barsalobres-Cavallari

et al. 2018

Plant Cell Rep

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A seed maturation protein gene (CaSMP) from Coffea arabica is expressed in the endosperm of yellow/green fruits. The CaSMP promoter drives reporter expression in the seeds of immature tomato fruits. In this report, an expressed sequence tag-based approach was used to identify a seed-specific candidate gene for promoter isolation in Coffea arabica. The tissue-specific expression of the cognate gene (CaSMP), which encodes a yet uncharacterized coffee seed maturation protein, was validated by RT-qPCR. Additional expression analysis during coffee fruit development revealed higher levels of CaSMP transcript accumulation in the yellow/green phenological stage. Moreover, CaSMP was preferentially expressed in the endosperm and was down-regulated during water imbibition of the seeds. The presence of regulatory cis-elements known to be involved in seed- and endosperm-specific expression was observed in the CaSMP 5'-upstream region amplified by genome walking (GW). Additional histochemical analysis of transgenic tomato (cv. Micro-Tom) lines harboring the GW-amplified fragment (~ 1.4 kb) fused to uidA reporter gene confirmed promoter activity in the ovule of immature tomato fruits, while no activity was observed in the seeds of ripening fruits and in the other organs/tissues examined. These results indicate that the CaSMP promoter can be used to drive transgene expression in coffee beans and tomato seeds, thus representing a promising biotechnological tool.

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CaPrx, a Coffea arabica gene encoding a putative class III peroxidase induced by root-knot nematode infection

Cited by 20 publications

References 38 publications

Intersection of transfer cells with phloem biology—broad evolutionary trends, function, and induction

Intersection of transfer cells with phloem biology—broad evolutionary trends, function, and induction

Seed-Specific Stable Expression of the α-AI1 Inhibitor in Coffee Grains and the In Vivo Implications for the Development of the Coffee Berry Borer

Identification of a seed maturation protein gene from Coffea arabica (CaSMP) and analysis of its promoter activity in tomato

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