Caprine toxoplasmosis in Southern Brazil: a comparative seroepidemiological study between the indirect immunofluorescence assay, the enzyme-linked immunosorbent assay, and the modified agglutination test

Fortes, Maira Salomão; Lopes-Mori, Fabiana Maria Ruiz; Caldart, Eloiza Teles; Constantino, Caroline; Evers, Fernanda; Pagliari, Sthefany; Almeida, Jonatas Campos de; Barros, Luiz Daniel de; Freire, Roberta Lemos; Garcia, João Luís; Headley, Selwyn Arlington; Navarro, Italmar Teodorico

doi:10.1007/s11250-017-1450-1

Cited by 21 publications

(6 citation statements)

References 32 publications

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“…Regarding female goats in Egypt, a seropositivity of 46.6% was recorded (Table 2) which was higher than that of Fortes et al (2017) in Brazil (33.3%), Tavakoli Kareshk et al (2017) in Iran (13-30%), Zou et al (2015) in China (17.6%), Issa (2017) in Iraq (13.04%) and Hossain et al (2018) in Bangladesh (6.66%).…”

Section: Discussionmentioning

confidence: 88%

Seroprevalence, isolation, molecular detection and genetic diversity of Toxoplasma gondii from small ruminants in Egypt

et al. 2018

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Toxoplasmosis is an infectious zoonotic disease caused by protozoan Toxoplasma gondii. Detection of T. gondii infection with touchy and particular strategies is a key advance to control and prevent toxoplasmosis. Genotyping can explain the virulence, epidemiology and setting up new methodologies for diagnosis and control in human and animals. The point of this study was to assess the seroprevalence of T. gondii in sheep and goat in Egypt and to comprehend the genetic variety of T. gondii isolates circling in Egypt. Blood samples were gathered from 113 ewes and 95 she-goats from three Egyptian governorates (Cairo, Giza and Al-Sharkia). Also blood and tissue samples were gathered from 193 sheep and 51 goats from Cairo and Giza abattoirs. All samples were assayed serologically utilizing ELISA and OnSite Toxo IgG/IgM Rapid test cassettes (OTRT) tests and the tissue samples of the seropositive animals were digested and microscopically examined then bio-assayed in mice as viability test. All the T. gondii isolates undergo molecular identification using PCR and genotyped utilizing nPCR/RFLP analysis of SAG2 gene. The total seropositivity of live sheep and goat was 47.15 and 39.2% utilizing ELISA and OTRT respectively. Concerning abattoirs, seropositivity, positive microscopic examination, mice viability from sheep samples were 47.1%, 37.3% and 44.1% respectively while that of goats were 45.5%, 33.3% and 48.6% respectively. Eighteen T. gondii isolates were affirmed utilizing PCR. Genotyping confirmed 10 isolates (55.5%) as type II, 6 (33.3%) as type III and 2 (11.1%) as atypical genotypes. Type II and III are the genotypes mostly circling among small ruminants in Egypt and this is most significance for the public health in Egypt.

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Section: Discussionmentioning

confidence: 88%

Seroprevalence, isolation, molecular detection and genetic diversity of Toxoplasma gondii from small ruminants in Egypt

et al. 2018

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“…This result was consistent with previous studies in sheep and goats in which male animals had a lower T. gondii prevalence than the females ( 42 , 43 ). Some studies inferred that higher seroprevalence in females might be associated with their longer life for milk production and reproduction, whereas males are slaughtered for meat supply at an earlier age ( 24 , 44 ). In addition, a previous study indicated that hormone differences may increase their susceptibility to T. gondii ( 45 ).…”

Section: Discussionmentioning

confidence: 99%

Seroprevalence and risk factors of Toxoplasma gondii and Neospora caninum infection in black goats in Yunnan Province, Southwestern China

Xie

Liang

et al. 2022

Front. Vet. Sci.

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Toxoplasma gondii and Neospora caninum are two obligate intracellular protozoan parasites that can cause reproductive failure and production losses. To date, there is no data of T. gondii and N. caninum seroprevalence in black goats in Yunnan Province, southwestern China. In the present study, a total of 734 serum samples were collected from black goats in four different counties of Yunnan Province. 734 and 590 serum samples were examined for antibodies against T. gondii and N. caninum by using MAT and indirect ELISA, respectively. A total of 123 and 76 samples were T. gondii-positive and N. caninum-positive, respectively. The overall seroprevalence of T. gondii in black goats was 16.76% (123/734, 95% CI: 14.06–19.46) with the titer ranged from 1:25 to 1:3200. The seroprevalence of N. caninum was 12.88% (76/590, 95% CI: 10.18–15.58). There was significant difference in seroprevalence of N. caninum in different regions (P < 0.01, χ2 = 30.63) and age groups (P < 0.05, χ2 = 11.85). Significant differences in seroprevalence of T. gondii were observed in different regions (P < 0.05, χ2 = 9.21) and different gender groups (P < 0.01, χ2 = 12.29). Results of seroprevalence of T. gondii and N. caninum indicated that T. gondii and N. caninum were prevalent parasites in black goats in Yunnan Province. This is the first report of seroprevalence of T. gondii and N. caninum in black goats in Yunnan Province. The results of this study indicated that some measures should be taken to control these two parasites and to reduce economic losses to the livestock industry in Yunnan Province.

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“…To calculate the sample size (n), an infinite population was used as a reference using EpiInfo 7.2.3.1. The estimated prevalence were based on the study by Fortes et al (2017), in which T. gondii was estimated to have a prevalence of 33.3%, and an error of 4%, resulting in a sample size of 533 animals, and the study by Figliuolo et al (2004), who estimated a prevalence of 6.4% and an error of 2% for N. caninum, resulting in a sample size of 575 animals.…”

Section: Methodsmentioning

confidence: 99%

Toxoplasma gondii and Neospora caninum infections and factors associated in goats in the Parana state, Southern Brazil

Romanelli

Matos

Ferreira

et al. 2020

Rev. Bras. Parasitol. Vet.

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The aim of this study was to determine the occurrence of anti-Toxoplasma gondii and anti-Neospora caninum antibodies and their associated factors among goats in farms from the Paraná state, South Brazil. The serological analysis was performed by indirect ELISA on 629 goat serum samples collected from 32 farms distributed in five mesorregions from the state. Seropositivity was observed in 30.7% of the animals for T. gondii, 6.3% for N. caninum, and 3.0% for both agents. Final multiple regression models showed that the use of assisted reproduction decreased the chance of seropositivity for T. gondii (PR=0.70) and the slaughtering site on the farm (PR=2.03) increased it. To N. caninum, the supplying concentrated feed to animals (OR=4.80) and animal pre-weaning death (OR=9.96) increased the chance of seropositivity. The variables associated with these seropositivities suggest deficiencies in sanitation and reproductive management. In addition, it recognizes critical points of infection for animal production management.

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Caprine toxoplasmosis in Southern Brazil: a comparative seroepidemiological study between the indirect immunofluorescence assay, the enzyme-linked immunosorbent assay, and the modified agglutination test

Cited by 21 publications

References 32 publications

Seroprevalence, isolation, molecular detection and genetic diversity of Toxoplasma gondii from small ruminants in Egypt

Seroprevalence, isolation, molecular detection and genetic diversity of Toxoplasma gondii from small ruminants in Egypt

Seroprevalence and risk factors of Toxoplasma gondii and Neospora caninum infection in black goats in Yunnan Province, Southwestern China

Toxoplasma gondii and Neospora caninum infections and factors associated in goats in the Parana state, Southern Brazil

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