Capilliposide from Lysimachia capillipes promotes terminal differentiations and reverses paclitaxel resistance in A2780T cells of human ovarian cancer by regulating Fos/Jun pathway

Zhang, Ke; Ying, Han-yue; Zhao, Rugang; Chen, Yuanyuan; Deng, Qinghua

doi:10.1016/j.chmed.2021.09.009

Cited by 6 publications

(5 citation statements)

References 22 publications

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“…Then, the cells were further incubated for 24 h and treated with the apoptosis detection kit of Annexin V-FITC/PI (Meilunbio, Dalian, China) according to the manufacturer′s instructions. The results were determined by a flow cytometer (BD FACSVerse, USA) …”

Section: Materials and Methodsmentioning

confidence: 99%

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Combination Nano-Delivery Systems Remodel the Immunosuppressive Tumor Microenvironment for Metastatic Triple-Negative Breast Cancer Therapy

Bai,

Liu,

You

et al. 2024

Mol. Pharmaceutics

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Triple-negative breast cancer (TNBC) is an aggressive type of breast cancer for which effective therapies are lacking. Targeted remodeling of the immunosuppressive tumor microenvironment (TME) and activation of the body’s immune system to fight tumors with well-designed nanoparticles have emerged as pivotal breakthroughs in tumor treatment. To simultaneously remodel the immunosuppressive TME and trigger immune responses, we designed two potential therapeutic nanodelivery systems to inhibit TNBC. First, the bromodomain-containing protein 4 (BRD4) inhibitor JQ1 and the cyclooxygenase-2 (COX-2) inhibitor celecoxib (CXB) were coloaded into chondroitin sulfate (CS) to obtain CS@JQ1/CXB nanoparticles (NPs). Then, the biomimetic nanosystem MM@P3 was prepared by coating branched polymer poly(β-amino ester) self-assembled NPs with melittin embedded macrophage membranes (MM). Both in vitro and in vivo, the CS@JQ1/CXB and MM@P3 NPs showed excellent immune activation efficiencies. Combination treatment exhibited synergistic cytotoxicity, antimigration ability, and apoptosis-inducing and immune activation effects on TNBC cells and effectively suppressed tumor growth and metastasis in TNBC tumor-bearing mice by activating the tumor immune response and inhibiting angiogenesis. In summary, this study offers a novel combinatorial immunotherapeutic strategy for the clinical TNBC treatment.

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Section: Materials and Methodsmentioning

confidence: 99%

“…The results were determined by a flow cytometer (BD FACSVerse, USA). 41 Wound-Healing Assay. HUVECs at a density of 2 × 10 5 cells/well were seeded in the 6-well plates and then cultured for 24 h. Afterward, a linear wound was created across the middle of the well surface by the pipet tip.…”

Section: Cells Andmentioning

confidence: 99%

Combination Nano-Delivery Systems Remodel the Immunosuppressive Tumor Microenvironment for Metastatic Triple-Negative Breast Cancer Therapy

Bai,

Liu,

You

et al. 2024

Mol. Pharmaceutics

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“…The colony formation assay was determined using a previously described method (Zhang et al. 2022 ). CNE2 cells were seeded in 12-well plates at a density of 3 × 10 5 cells/well and left overnight in an incubator at 37 °C.…”

Section: Methodsmentioning

confidence: 99%

Lucialdehyde B suppresses proliferation and induces mitochondria-dependent apoptosis in nasopharyngeal carcinoma CNE2 cells

Liu

Yu²,

Chen

et al. 2023

Pharmaceutical Biology

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Context Lucialdehyde B (LB), an effective triterpenoid isolated from Ganoderma lucidum (Leyss. ex Fr.) Karst. (Polyproraceae), exerts cytotoxic activity against nasopharyngeal carcinoma CNE2 cells. Objective To investigate the antiproliferative and pro-apoptotic effects of LB on CNE2 cells and explore its underlying mechanisms. Materials and methods LB concentrations of 5–40 μg/mL were used. Cell proliferation was determined using MTT, CFSE, and colony formation assays. LB-induced apoptosis and cell cycle arrest were measured by flow cytometry after 48-h LB treatments. Fluorescence microscopy and flow cytometry were performed to measure the alteration of MMP, mPTP opening, ROS level, and Ca 2+ content in CNE2 cells. Western blotting was performed to evaluate the expression of mitochondrial apoptosis-related and Ras/ERK signaling proteins. Results IC 50 values of LB against CNE2 cells for 24, 48, and 72 h were 25.42 ± 0.87, 14.83 ± 0.93, and 11.60 ± 0.77 μg/mL, respectively. The CFSE assay showed that the cell proliferation index was 12.70 in the LB treatment group and 31.44 in the control group. LB significantly reduced clonogenic capacity, promoted cell apoptosis and induced cell cycle arrest at the G2/M phase. Our observations also revealed that LB induced ROS and calcium aggregation, opening of mPTP, MMP reduction, upregulation of mitochondrial apoptosis-related protein expression and inhibition of Ras/ERK signaling cascades. Discussion LB suppresses proliferation and induces mitochondrial-dependent apoptosis in nasopharyngeal carcinoma CNE2 cells. Conclusions LB may have a potential use as a clinical drug candidate for nasopharyngeal carcinoma treatment.

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“…Bioactive compounds in TCMs such as flavonoids ( Qin et al, 2022 ), saponins ( Zhang, Ying, Zhao, Chen, & Deng, 2022 ), alkaloids ( Feng, Ju, Chen, Li, & Wang, 2022 ), diterpenoids ( Hou, Yao, & Song, 2021 ), and polysaccharides ( Guo et al, 2022 ) are mostly non-volatile and require appropriate separation methods using liquid chromatography (LC). In many cases, conventional 1D-LC is not sufficient to fully separate target components in one run due to its limited peak capacity and separation efficiency, which is insufficient for the analysis of complex TCMs.…”

Section: Mdlc In Tcms Fieldmentioning

confidence: 99%

Gain deeper insights into traditional Chinese medicines using multidimensional chromatography combined with chemometric approaches

Yang,

Zeng,

Wen

et al. 2024

Chinese Herbal Medicines

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Capilliposide from Lysimachia capillipes promotes terminal differentiations and reverses paclitaxel resistance in A2780T cells of human ovarian cancer by regulating Fos/Jun pathway

Cited by 6 publications

References 22 publications

Combination Nano-Delivery Systems Remodel the Immunosuppressive Tumor Microenvironment for Metastatic Triple-Negative Breast Cancer Therapy

Combination Nano-Delivery Systems Remodel the Immunosuppressive Tumor Microenvironment for Metastatic Triple-Negative Breast Cancer Therapy

Lucialdehyde B suppresses proliferation and induces mitochondria-dependent apoptosis in nasopharyngeal carcinoma CNE2 cells

Gain deeper insights into traditional Chinese medicines using multidimensional chromatography combined with chemometric approaches

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