Capillary zone electrophoretic separations of proteins in polyethylene glycol-modified capillaries

Bruin, Gerard; Chang, Jiang; Kuhlman, R.H.; Zegers, K.; Kraak, J.C.; Poppe, H.

doi:10.1016/s0021-9673(00)94190-6

Cited by 282 publications

(81 citation statements)

References 11 publications

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“…It is, however, still a matter of discussion, which is reflected by the number of contradictory contributions published on the subject. In some of them, the "overall" mobility (including electroosmosis) is measured as a function of buffer concentration [8,9]. An exponential decrease with increasing ionic strength was found, attributed mainly to a change in electroosmosis.…”

Section: Introductionmentioning

confidence: 99%

Ionic strength and charge number correction for mobilities of multivalent organic anions in capillary electrophoresis

Friedl

Reijenga

Kenndler

1995

Journal of Chromatography A

126

105

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An empirical expression was derived for the actual mobility, ix, valid in an extended range of ionic strength, I, between 0.001 and 0.1 mol/1, using a three-parameter exponential fit for the ratio of actual and absolute mobilities (ix0 at I = 0), which consists of two variables: ionic strength of the buffer and charge number, z, of the solute. The charge numbers of the solute anions (21 aromatic sulfonates) ranged between 1 and 6, and the ionic strength was varied in the range mentioned using a sodium acetate buffer. The mobilities were determined at 25.0°C in a coated capillary with suppressed electroosmotic flow. The resulting fitting equation, valid under these conditions, is ix/ix0 = exp(-0.77X zz/). The difference between measured mobilities and mobilities calculated from a tenfold higher or lower ionic strength, using this equation, is within 5% for all experiments. For multivalent ions, ix values can be derived from ixo with comparable accuracy.

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Section: Introductionmentioning

confidence: 99%

Ionic strength and charge number correction for mobilities of multivalent organic anions in capillary electrophoresis

Friedl

Reijenga

Kenndler

1995

Journal of Chromatography A

126

105

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“…The use of polymeric coatings has led to high efficiency and reproducible protein separations preventing the surface adsorptions. Polymers ranging from highly hydrophobic to highly hydrophilic were used for this purpose to obtain efficient separations of proteins in CE [1][2][3][4][5][6][7][8][9][10][11][12][13][14][15][16].…”

Section: Introductionmentioning

confidence: 99%

Selectivity change in the separation of proteins and peptides by capillary electrophoresis using high-molecular-mass polyethyleneimine

Cifuentes

Poppe

Kraak

et al. 1996

Journal of Chromatography B: Biomedical Sciences and Applicatio

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“…normal peak shapes appear, there is no overloading and the undesired adsorption can be neglected. Similar effects occur in the separation of proteins (because of denaturation and adsorption) [2], and in capillary electrophoresis (sample overloading and adsorption) [3,4].…”

Section: Introductionmentioning

confidence: 83%

Linearization of column responses by correlation chromatography. Separation improvement and reduction of undesired adsorption effects

Louwerse

Smit

1994

Chromatographia

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SummaryExperiments and simulations prove that correlation chromatography can greatly reduce the disadvantage of a non-linear response of the chromatographic column. A factor that has been accepted as being an important source of error in correlation or multiplex chromatography, has been shown not to be. Separations affected, improve dramatically when correlation chromatography is used, and a substantial amount of correlation noise only arises when there is a large difference in separation between a conventional chromatogram and a correlogram. A model has been developed for simulating these nonlinearities. It is shown that, especially for correlation chromatography, the simulation results match the practical measurements very well.

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Capillary zone electrophoretic separations of proteins in polyethylene glycol-modified capillaries

Cited by 282 publications

References 11 publications

Ionic strength and charge number correction for mobilities of multivalent organic anions in capillary electrophoresis

Ionic strength and charge number correction for mobilities of multivalent organic anions in capillary electrophoresis

Selectivity change in the separation of proteins and peptides by capillary electrophoresis using high-molecular-mass polyethyleneimine

Linearization of column responses by correlation chromatography. Separation improvement and reduction of undesired adsorption effects

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