“…For the ACE experiments, an adapted home-made CE apparatus [26] equipped with a UV photometric detector monitoring absorbance at 206 nm was used. The ACE separations were performed in the internally uncoated fused silica capillary with an outer polyimide coating, a total/effective length of 306/200 mm, an id/od of 50/375 m. The separations were carried out at ambient temperature, 23-26 • C. A Clarity chromatography and electrophoresis station (DataApex, Prague, CR) was used for data acquisition and evaluation, and the Origin 6.1 program (OriginLab Corp., Northampton, MA, USA) was employed for the non-linear regression analysis.…”