Capillary Zone Electrophoresis of some organic acids in milk whey

Abstract

This paper describes a method for analysing some acids in milk whey by Capillary Electrophoresis.After eliminating of whey proteins by ultrafiltration, the whey underwent electrophoretic separation in the presence of anodic electrophoretic flow. The following analytes were detected:citric, orotic, uric and hippuric acids. A procedure is described for sample preparation and the operating conditions for electrophoretic capillary separation established. Finally, orptic acid is quantitatively determined

Recent advances in the application of capillary electromigration methods for food analysis

“…Similar approach was used by Buiarelli et al [111] to analyze citric, orotic, uric and hippuric acids in whey, concluding that CE is a quick and easy technique for determination of milk whey acids. CE-UV also provided the simultaneous determination of arsenite, arsenate, monomethylarsonic acid, dimethylarsinic acid, selenate, selenite, selenocystine, selenomethionine and selenocystamine at 195 nm [112].…”

“…Analysis of small ions by CE is also an active area in food analysis [108][109][110][111][112][113][114][115][116][117][118][119]. In general, two main problems have to be solved when analyzing small ions by CE.…”

A review of the analytical methods to determine organic acids in grape juices and wines

“…In recent years, liquid chromatography has widely replaced by capillary electrophoresis for the determination of organic acids in very different matrix as clinical samples García & Barbas, 2003;García, Barbas, Aguilar, & Castro, 1998), environmental samples (Ahumada, Mendoza, Escudero, Mossert, & Ascar, 2001;Li, Huang, & Shan, 2003;Naidu & Chen, 2001;Wang, Qu, Shan, & Lin, 2003;Xu, Chen, Yu, & Tang, 2001) or food samples (Buiarelli, Cartoni, Coccioli, & Jasionowska, 2003;Cortacero-Ramírez, Segura-Carretero, Hernáiz-Bermú dez de Castro, & Fernández-Gutiérrez, 2005;Galli & Barbas, 2004;Izco, Tormo, & Jiménez-Flores, 2002a, Izco, Tormo, & Jiménez-Flores, 2002bKlampfl, 1999;Roselló , Galiana-Balaguer, Herrero-Martínez, Maquieira, & Nuez, 2002;Saavedra, García, & Barbas, 2000;Saavedra, Rupérez, & Barbas, 2001;Xiao-Yun, Jian-De, & Yao-Zu, 1997). This great development is because capillary electrophoresis is a technique with a lot of advantages like high resolution, simplicity and automation, short analysis times, low consumption of reagents and samples, and minimum preparation of sample even in complex matrices.…”

Determination of organic acids by CE and CEC methods

“…Therefore, a substantial number of publications dealing with this topic can be found in the literature, as can be seen from recent review articles [5][6][7]. An overview of CE applications in this field, published since 2003, is given in Table 1. CE and CEC methods for the determination of organic acids in a variety of beverages including fruit juices [27][28][29][30][31][32], beer [32,33], wines [27,30,[34][35][36][37][38], milk [39], and coffee [40,41] have been reported in the recent literature. Using a CZE method with coelectroosmotic conditions, Mato et al [27] succeeded in the simultaneous analysis of 16 organic acids in beverages in less than 3.5 min.…”

“…For this purpose the pyroglutamate and the caffeine content of different types coffee (including several varieties of lyophilized coffee) were determined using an MEKC method. Buiarelli et al [39] described the analysis of several organic acids including orotic acid an important precursor of uracil and cytosine in the biosynthesis of pyrimidinic nucleotides in milk whey. Their method includes a protocol for sample preparation and electrophoretic separation of milk samples.…”