Capillary zone electrophoresis of soil humic acid fractions obtained by coupling size-exclusion chromatography and polyacrylamide gel electrophoresis

Cavani, Luciano; Ciavatta, Claudio; Trubetskaya, O. E.; Reznikova, O. I.; Afanas'eva, G. V.; Trubetskoj, Oleg

doi:10.1016/s0021-9673(02)01737-5

Cited by 17 publications

(6 citation statements)

References 24 publications

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“…At present, various techniques such as chromatographic techniques based on molecular size (e.g., size exclusion chromatography and gel permeation chromatography), ultrafiltration, reversed-phase highperformance liquid chromatography (RP-HPLC), capillary electrophoresis and flow field flow fractionation have been used to separate and characterize HAs [15][16][17]. Additionally, the structure properties such as polarity and aromaticity of the obtained HAs fractions are diverse, and thus their photoinductive activities would be different from each other.…”

Section: Introductionmentioning

confidence: 99%

Effects of humic acid fractions with different polarities on photodegradation of 2,4-D in aqueous environments

Quan

et al. 2008

Front. Environ. Sci. Eng. China

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Four fractions (A, B, C, and D) of humic acids (HAs) were separated based on the polarity from weak to strong. UV-vis absorption and Fourier transform infrared spectroscopy (FTIR) analysis show that the fractions C and D possessed more aromatic C5C content. The influences of HAs and their fractions on the photolysis were investigated by the photodegradation of 2,4-D solutions under simulated solar light irradiation. The degradation rate of 2,4-D was found to decrease in the presence of bulk HAs or their fractions especially at high HAs concentration. The fractions of strong polarity C and D retarded the degradation rate more than the fractions of weak polarity A and B. This could be attributed to the different absorption intensity of the four HAs fractions in the order of D > C . A . B, and the stronger p-p electron donor-acceptor interactions between the strong polar fractions and 2,4-D.

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Section: Introductionmentioning

confidence: 99%

Effects of humic acid fractions with different polarities on photodegradation of 2,4-D in aqueous environments

Quan

et al. 2008

Front. Environ. Sci. Eng. China

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“…[8]. A difficult problem is also the sorption of HM onto uncoated silica capillaries, and for that reason relatively high sample concentrations or long injection times are usually needed.…”

Section: Introductionmentioning

confidence: 99%

Comparative study for separation of aquatic humic substances by capillary zone electrophoresis using uncoated, polymer coated and gel-filled capillaries

Peuravuori¹,

Lepane²,

Lehtonen³

et al. 2004

Journal of Chromatography A

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“…The pattern of humic substances mainly depends on sample structure, buffer composition and other experimental conditions. In the case of the fused-silica capillary, humic acid samples afford characteristic regions in the electropherogram: a spike (low molecular compounds) and a wide ''humic hump'' (sometimes with poorly defined shoulders) from heterogeneous matter with poorly defined structure [31][32][33]. However, analysis of humic substances in the bare fused-silica capillary is complicated due to the adsorption of the sample on the capillary wall.…”

Section: Humic Acidsmentioning

confidence: 99%

CE separation of various analytes of biological origin using polyether ether ketone capillaries and contactless conductivity detection

2009

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The development of efficient and sensitive analytical methods for the separation, identification and quantification of complex biological samples is continuously a topic of high interest in biological science. In the present study, the possibility of using a polyether ether ketone (PEEK) capillary for the CE separation of peptides, proteins and other biological samples was examined. The performance of the tubing was compared with that of traditional silica capillaries. The CE analysis was performed using contactless conductivity detection (C(4)D), which eliminated any need for the detection window and was suitable for the detection of optically inactive compounds. In the PEEK capillary the cathodic EOF was low and of excellent stability even at extremes pH. In view of this fast biological anions were analyzed using an opposite end injection technique without compromising separation. A comparison of the performances of fused-silica and polymer capillaries during the separation of model sample mixtures demonstrated the efficiency and separation resolution of the latter to be higher and the reproducibility of the migration times and peak areas is better. Furthermore, PEEK capillaries allowed using simple experimental conditions without any complicated modification of the capillary surface or use of an intricate buffer composition. The PEEK capillaries are considered as an attractive alternative to the traditional fused-silica capillaries and may be used for the analysis of complex biological mixtures as well as for developing portable devices.

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Capillary zone electrophoresis of soil humic acid fractions obtained by coupling size-exclusion chromatography and polyacrylamide gel electrophoresis

Cited by 17 publications

References 24 publications

Effects of humic acid fractions with different polarities on photodegradation of 2,4-D in aqueous environments

Effects of humic acid fractions with different polarities on photodegradation of 2,4-D in aqueous environments

Comparative study for separation of aquatic humic substances by capillary zone electrophoresis using uncoated, polymer coated and gel-filled capillaries

CE separation of various analytes of biological origin using polyether ether ketone capillaries and contactless conductivity detection

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