Capillary Zone Electrophoresis of Serum Proteins: Effects of Changed Analytical Conditions

Abstract

Analytical conditions in a system for capillary zone electrophoresis (Beckman Paragon CZE 2000) were originally selected to allow serum protein separation into five discrete protein zones, corresponding to those of conventional clinical electrophoresis. To improve the system's performance, new analytical conditions have been made available. We compared the two sets of conditions ("new" = y; "old" = x) for possible variations of results caused by the change. One hundred thirteen serum samples, covering wide intervals of values, were assayed on two twin instruments working under the old and the new conditions; results were assessed statistically and graphically. Possible clinical significance of differences was checked by comparison with the biological variation-based quality specifications for bias. Statistically significant (y-x) differences were observed for the alpha1-, alpha2- and beta-globulin zones; clinically significant differences were observed for all the zones, with the exception of the gamma-globulin zone. Therefore, old/new regression equations were calculated, whose reliability was assured by the wide interval of values, by the large sample size, and by the low dispersion of single values around the mean concordance estimates. Such equations may be used to convert "old" into "new" reference values, and for the intercomparison of patient results obtained under different analytical conditions.

High‐density lipoproteins contribute to the α‐1‐globulin zone in capillary electrophoresis of human serum proteins

“…CZE assays were carried out with two twin Paragon CZE 2000 instruments (Beckman Coulter, Milano, Italy), a dedicated system for clinical serum protein assay, based on a seven-capillary separation apparatus and 214 nm monitoring of the effluent. The two instruments were equipped with the software release 1.6 [14]. The instruments were fed the total protein values of the assayed sera, for automatic calculation of the zones' protein mass concentration, in g/L.…”

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