Capillary zone electrophoresis for the characterization of latex particles

Vanhoenacker, Gerd; Goris, Ludwig; Sandra, Pat

doi:10.1002/1522-2683(200107)22:12<2490::aid-elps2490>3.0.co;2-b

Cited by 15 publications

(29 citation statements)

References 34 publications

(23 reference statements)

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“…Among these techniques is capillary electrophoresis (CE) which has been used in the analysis of viruses and bacteria [3,4], liposomes [5], gold colloid [6], and polymer microspheres [7]. Among these particles gold colloid and microspheres are expected to be homogeneous and thus good models to study the electrophoretic behavior of particles in the presence of an electric field.…”

Section: Introductionmentioning

confidence: 78%

“…Multiple reports have clearly confirmed that polystyrene latex microspheres have an intrinsic electrophoretic mobility that makes them amenable to analysis by CE [7,11]. This fact is confirmed in Fig.…”

Section: Electropherograms Of Individually Detected Microspheresmentioning

confidence: 96%

“…1, that shows electropherograms of two buffer systems, borate-SDS and borate, resulting from sampling electrokinetically or by siphoning a few nanoliters of a microsphere suspension containing from one to ten microspheres. As opposed to the previously reported Gaussian-like profiles resulting from the detection of millions of microspheres [7,11], in this report each microsphere is detected individually. In the postcolumn LIF detector, which typically allows for the detection of less than 600 molecules [5], an argon-ion laser focused about 50 mm away from the capillary tip excites each microsphere as it leaves the capillary and is washed away by a sheath flow.…”

Section: Electropherograms Of Individually Detected Microspheresmentioning

confidence: 99%

“…This function increases monotonically between the kR range 1-100. Therefore, both z potential and f(kR) help explain the observed electrophoretic properties of particles with z potential lower than 25 mV and with kR lower than 100 [7].…”

Section: Introductionmentioning

confidence: 95%

“…By minimizing the contribution to broadening related to the analytical procedure, several researchers have reported that electrophoretic heterogeneity dispersion resulting from either size or surface composition is the ultimate limit in band broadening [7,9,11]. Unfortunately, in these reports the conclusions are based on the combined response of millions of particles which typically are convolutions of the instrumental response time and the number of events being detected at a given time.…”

Section: Introductionmentioning

confidence: 99%

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Determination of individual microsphere properties by capillary electrophoresis with laser-induced fluorescence detection

2002

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Capillary electrophoresis with postcolumn laser-induced fluorescence detection was used to individually detect 6.0, 1.0, 0.5, and 0.2 num diameter polystyrene microspheres and individually measure their electrophoretic mobility. The analysis of a nanoliter-size volume from a microsphere suspension results in an electropherogram characterized by several narrow spikes in a well-defined migration time window. Each spike is associated with one microsphere because, when one single microsphere is introduced into the capillary by micromanipulation, the electropherogram has only one spike in the same migration time window. The distributions of individual measurements resulting from an electropherogram were used to evaluate the reproducibility from run to run, observe the effect of sodium dodecyl sulfate (SDS) added to the running buffer, and to investigate the origin of electrophoretic dispersion. As expected from the interactions between microspheres and SDS, the addition of this surfactant to the running buffer narrowed the range and shifted the average electrophoretic mobility to more negative values. After evaluating common sources of broadening in capillary electrophoresis, electrophoretic dispersion was attributed to microsphere heterogeneity. Unlike electropherograms displaying Gaussian-like profiles, the two-dimensional representations of the individual measurements provide a new alternative to evaluate and study electrophoretic-related properties of microspheres.

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Section: Introductionmentioning

confidence: 78%

Section: Electropherograms Of Individually Detected Microspheresmentioning

confidence: 96%

Section: Electropherograms Of Individually Detected Microspheresmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 95%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Determination of individual microsphere properties by capillary electrophoresis with laser-induced fluorescence detection

2002

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Separation and characterization of sub-μm- and μm-sized particles by capillary zone electrophoresis

2002

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The analytical separation and characterization of particles in the size range of sub-microm and microm diameters by capillary zone electrophoresis (CZE) has been reviewed. The theoretical basis, on which the mobility can be interpreted to provide information regarding characteristics of particle surface, has shortly been presented. Particular emphasis was put on the model dependence of that interpretation and the need in most applications to forego the classical idealized model of spherical particles with "smooth" surfaces and to apply more realistic models, which take the "hairy" surface of real particles into account. Some highlights of the literature on the CZE of polystyrene latex microspheres, organic and inorganic colloids, lipoprotein particles, viruses, liposomes, biological membrane vesicles, and biological cells have been discussed. Also summarized are the reports on the particle size dependence of mobility and peak broadening in CZE and on electrophoretic behavior of rodlike particles and particle aggregates. Finally, the effects of neutral polymers in the background electrolyte on particle mobility and peak width are reviewed.

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Separation of cationic polymer particles and characterization of avidin‐immobilized particles by capillary electrophoresis

2006

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Cationic polymer microparticles have received much attention especially in the field of biotechnology, such that their analysis and separation have become important. So far, the separation of cationic polymer particles with different size using CE has not been achieved and the cationic particles migrated as if they are negatively charged, probably due to electrostatic interaction between capillary wall and cationic polymer particles. In this paper, the separation of cationic polymer microparticles by CE was investigated in detail. The separation of cationic particles with different size was achieved in CE by taking into account the interaction between sample particles and the inner surface of capillaries. By employing a poly(vinyl alcohol)-coated capillary, a better size separation of amine-modified latex particles was obtained compared to a Polybrene-coated capillary. It was elucidated that the composition, concentration, and pH of the background solution were also important factors in the separation of colloidal particles to avoid the surface adsorption and the characteristic aggregation of polymer particles. Furthermore, the CE analysis was applied to the characterization of cationic protein-immobilized particles.

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Capillary zone electrophoresis for the characterization of latex particles

Cited by 15 publications

References 34 publications

Determination of individual microsphere properties by capillary electrophoresis with laser-induced fluorescence detection

Determination of individual microsphere properties by capillary electrophoresis with laser-induced fluorescence detection

Separation and characterization of sub-μm- and μm-sized particles by capillary zone electrophoresis

Separation of cationic polymer particles and characterization of avidin‐immobilized particles by capillary electrophoresis

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