Capillary Zone Electrophoresis Analysis of Anti-Epileptic Drugs

Abstract

This paper describes the development of a simple, reliable, reproducible, and fast method, based on capillary zone electrophoresis with diode array detection, for the separation and determination of two antiepileptic drugs. Phenytoin (DPH) and phenobarbital (PB) were separated in 4 min by using a carrier containing glycine 50 mM, pH 10.5. The limit of detection was 0.1 µg mL -1 for both compounds. Satisfactory reproducibility was obtained for migration times (<1.4%) and corrected peak areas (<6.9%) in the analysis of pharmaceutical formulations. The average recoveries obtained, which ranged between 100 and 108%, testify to the accuracy of the proposed method.

Computer aided-molecular design and synthesis of a high selective molecularly imprinted polymer for solid-phase extraction of furosemide from human plasma

“…HPLC methods have been the main approach [9][10][11][12][13][14][15]. Other techniques reported are monolithic silica rod liquid chromatography [16], micellar liquid chromatography [17], capillary electrophoresis [1,18,19], titrimetry [20], spectrophotometry [21][22][23], diffuse reflectance spectroscopy [24] and chemiluminescence [25]. All of these methods need a pre-extraction step, usually solid-phase extraction (SPE), for clean-up of matrix complexity prior to quantitative analysis.…”

FI-chemiluminometric study of thiazides by on-line photochemical reaction

“…However, as HCT often is combined with other drugs, methods of separation (chromatography [13,14] and electrophoresis [15,16]) have been suggested for its determination, just as multicomponent analysis based on derivative or multiwavelength spectrophotometry [17][18][19]. Methods devoted to obtain the dissolution profiles of hydrochlorothiazide [20] or the simultaneous dissolution profiles of this drug and captopril [21], have been also presented.…”

Validation of non-aqueous capillary electrophoresis for simultaneous determination of four tricyclic antidepressants in pharmaceutical formulations and plasma samples

“…As a matter of fact, it might be attributed to the relative limited number of validated quantitative analytical methods in CE. The analysis of highly hydrophobic and structurally similar compounds by CE often requires the use of special additives like surfactants or complexing agents in order to improve the separation selectivity [10][11][12][13]. Non-aqueous capillary electrophoresis (NACE) [14][15][16][17][18] has emerged as an alternative to obtain high-resolution separations of hydrophobic drugs without using the above-mentioned additives.…”