Capillary Methods for Drug Analysis

Hendrickx, Ans; Mangelings, Debby; Heyden, Yvan Vander

doi:10.1093/jaoac/94.3.667

Cited by 16 publications

(15 citation statements)

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“…When band broadening is limited, high peak efficiencies, which are important for complex samples, are achieved. Chiral separations also benefit from these increased efficiencies because better separations can be obtained with higher resolutions (Hendrickx et al, 2011a).…”

Section: Introductionmentioning

confidence: 99%

Three versus five micrometer chlorinated polysaccharide‐based packings in chiral capillary electrochromatography: efficiency and precision evaluation

Hendrickx

Klerck

Mangelings

et al. 2013

Biomedical Chromatography

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In an earlier part of this study (performance evaluation) it was observed, for home-made capillary electrochromatography (CEC) columns, that smaller particle diameters do not always generate higher efficiencies. This phenomenon was further examined in this study, evaluating Van Deemter curves. Naphthalene and trans-stilbene oxide were analyzed on four 3 µm and four 5 µm chlorinated polysaccharide-based chiral stationary phases (CSPs) applying voltages ranging from 5 to 30 kV. Neither the 3 nor the 5 µm packings generated systematically the highest efficiencies. The varying column efficiencies were optimized by evaluating nine packing procedures for both 3 and 5 µm CSPs. Again it was observed that smaller particle-size packings were not necessarily beneficial for the efficiency of the CEC analysis. This observation was statistically evaluated. A variability study evaluated different precision estimates related to column packing and replicate measurement conditions. The best columns with the highest efficiencies (for chiral separations) and good precision, that is, the lowest RSD values, were generated by the packing procedure in which an MeOH-slurry and a water rinsing step of 8 h were applied.

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Section: Introductionmentioning

confidence: 99%

Three versus five micrometer chlorinated polysaccharide‐based packings in chiral capillary electrochromatography: efficiency and precision evaluation

Hendrickx

Klerck

Mangelings

et al. 2013

Biomedical Chromatography

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“…It is a hybrid technique because the separation is based on both chromatographic partition and electrophoretic migration. The high numbers of theoretical plates which in principle can be obtained with CEC, because of the flat electroosmotic flow profile (limited band broadening), should be an advantage when a minor peak must be detected in the presence of a major, like in impurity profiling experiments (Hendrickx et al, 2011b). The application domains of CEC are quite similar to those of HPLC and CE, and include amongst others, the determination of drug impurities, active-component assays and chiral separations, of which the latter field is very actively and extensively investigated (Altria et al, 1998;He et al, 2009;Hendrickx et al, 2011b;Liu et al, 2012;Lv et al, 2011;Wu et al, 2011).…”

Section: Introductionmentioning

confidence: 99%

“…The high numbers of theoretical plates which in principle can be obtained with CEC, because of the flat electroosmotic flow profile (limited band broadening), should be an advantage when a minor peak must be detected in the presence of a major, like in impurity profiling experiments (Hendrickx et al, 2011b). The application domains of CEC are quite similar to those of HPLC and CE, and include amongst others, the determination of drug impurities, active-component assays and chiral separations, of which the latter field is very actively and extensively investigated (Altria et al, 1998;He et al, 2009;Hendrickx et al, 2011b;Liu et al, 2012;Lv et al, 2011;Wu et al, 2011). Moreover, compounds of different naturesincluding biomoleculescan be analyzed by CEC and analytes of interest can be determined at therapeutic levels in small sample volumes (Fu et al, 2003;Hendrickx et al, 2011b;Quaglia et al, 2002).…”

Section: Introductionmentioning

confidence: 99%

“…These aim at obtaining a baseline separation for partially separated enantiomers, decreasing the analysis time and/or improving the quality of the separation when a baseline separation has already been achieved, or inducing enantioselectivity when none was seen in the screening. Screening and optimization steps are defined based on experimental data and the literature (Hendrickx et al, 2011b).…”

Section: Introductionmentioning

confidence: 99%

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Three versus five micrometer chlorinated polysaccharide‐based packings in chiral capillary electrochromatography: performance evaluation

Hendrickx

Klerck

Mangelings

et al. 2013

Biomedical Chromatography

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In this study, a test set of 44 nonacidic compounds was analyzed on four 3 µm chlorinated polysaccharide-based chiral stationary phases with cellulose tris (3-chloro-4-methylphenylcarbamate) (Lux Cellulose-2®; LC2), amylose tris (5-chloro-2-methylphenylcarbamate) (Lux Amylose-2®, LA2), cellulose tris (4-chloro-3-methylphenylcarbamate) (Lux Cellulose-4®; LC4) and cellulose tris (3,5-dichlorophenylcarbamate) (Sepapak-5®, Sp5) as selectors. The analysis times, retention factors, efficiencies and enantioselectivities were compared with the results obtained on their 5 µm analogs. All 3 µm packings, except for LA2, individually separated more compounds than their 5 µm analogs. When the cumulative success rates on the 3 and 5 µm packings were considered, it was observed that they were similar for both particle sizes; the combination of three or four 5 µm columns separated one compound more from the considered test set than that of the same number of 3 µm columns. Furthermore, it was observed that the 3 and 5 µm packings showed some complementarity. Only four compounds were not separated on any of the columns, while the use of only either the 3 or 5 µm columns resulted in 10 and nine not-separated compounds, respectively. The analyses on 5 µm LC2 and Sp5 were faster than on their 3 µm analogs. For LC4 the 3 µm packing showed the shortest analysis times and diverse analysis times for both particle sizes were obtained on LA2. Furthermore, three out of four 3 µm packings, that is, LC2, LC4, and Sp5, were found to be more efficient than their 5 µm analogs.

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“…These analyses have been summarized elsewhere, for example, in [25][26][27][28] and are not included in the present review either. For earlier compilations of the use of capillary electromigration methods in drug analysis see, for example, [7,[10][11][12][29][30][31][32][33][34].…”

Section: Introductionmentioning

confidence: 99%

Recent advances in capillary electrophoretic migration techniques for pharmaceutical analysis

et al. 2014

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Since the introduction about 30 years ago, CE techniques have gained a significant impact in pharmaceutical analysis. The present review covers recent advances and applications of CE for the analysis of pharmaceuticals. Both small molecules and biomolecules such as proteins are considered. The applications range from the determination of drug-related substances to the analysis of counterions and the determination of physicochemical parameters. Furthermore, general considerations of CE methods in pharmaceutical analysis are described.

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Capillary Methods for Drug Analysis

Cited by 16 publications

References 0 publications

Three versus five micrometer chlorinated polysaccharide‐based packings in chiral capillary electrochromatography: efficiency and precision evaluation

Three versus five micrometer chlorinated polysaccharide‐based packings in chiral capillary electrochromatography: efficiency and precision evaluation

Three versus five micrometer chlorinated polysaccharide‐based packings in chiral capillary electrochromatography: performance evaluation

Recent advances in capillary electrophoretic migration techniques for pharmaceutical analysis

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