Capillary electrophoretic profiling and pattern recognition analysis of urinary nucleosides from uterine myoma and cervical cancer patients

Kim, Kyoung-Rae; La, Sookie; Kim, Ahrrum; Kim, Jung-Han; Liebich, H.M.

doi:10.1016/s0378-4347(00)00585-5

Cited by 46 publications

(41 citation statements)

References 20 publications

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“…The mean levels of 14 nucleosides in normal urines have been established (Table 5). The results were well correlated with those obtained by other authors [14,18,19,23]. Fig.…”

Section: Resultssupporting

confidence: 91%

“…Several methodologies, including gas chromatography (GC) [3], reversed-phase high-performance liquid chromatography (RP-HPLC) [4,5,9,[15][16][17][19][20][21], capillary electrophoresis (CE) [7][8][9]22,23] and immunoassays [6,[24][25][26] have been used for the analysis of urinary nucleosides. Although immunoassays allow rapid analysis of hundreds of samples a day, the lack of specificity is of concern in evaluating the accuracy of the quantitative data.…”

Section: Introductionmentioning

confidence: 99%

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Determination of urinary nucleosides by direct injection and coupled-column high-performance liquid chromatography

Zheng

Yang

et al. 2005

Journal of Chromatography B

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“…The mean levels of 14 nucleosides in normal urines have been established (Table 5). The results were well correlated with those obtained by other authors [14,18,19,23]. Fig.…”

Section: Resultssupporting

confidence: 91%

Section: Introductionmentioning

confidence: 99%

Determination of urinary nucleosides by direct injection and coupled-column high-performance liquid chromatography

Zheng

Yang

et al. 2005

Journal of Chromatography B

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“…Each urine sample was analyzed in triplicate, and the results are summarized in Table 3. In the report from Kim and co-workers, 9 who used capillary electrophoresis with a photodiode array detector, urinary concentrations of pseudouridine and 3-methyluridine in healthy subjects were given as 14.80 and 0.29 nmol/mmol creatinine, respectively. These values are similar to our results (13.47 and 0.39 nmol/mmol creatinine, respectively).…”

Section: Application Of the Methodsmentioning

confidence: 99%

“…[1][2][3][4][5] Various methods have been developed for quantifying modified nucleosides, based on an enzyme-linked immunosorbent assay (ELISA), 6,7 32 P-post-labelling, 8 capillary electrophoresis, 9,10 HPLC with photodiode array detection 11,12 or ECD, 13 and GC/MS. [14][15][16] However, GC/MS analysis faced additional problems in the hydrolysis and derivatization steps.…”

mentioning

confidence: 99%

A rapid and sensitive method for quantitation of nucleosides in human urine using liquid chromatography/mass spectrometry with direct urine injection

Lee

Jung

Kim

et al. 2004

Rapid Comm Mass Spectrometry

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Oxidized nucleosides are biochemical markers for tumors, aging, and neurodegenerative diseases. However, during the last decade, the analytical methods for nucleosides by gas chromatography/ mass spectrometry (GC/MS) and high-performance liquid chromatography (HPLC) with singleparameter detectors like electron-capture detection (ECD) have not been sufficiently rapid or reliable to detect nucleosides in urine and to analyze clinical samples. It has been reported (Dudley et al., Rapid Commun Mass Spectrom. 2000; 14: 1200) that liquid chromatography with electrospray mass spectrometry (LC/ESI-MS) is more specific and sensitive for analysis of nucleosides than HPLC with conventional detectors; however, this method required complex extraction steps. In the present work a direct LC/ESI-MS method for nucleosides without extraction of urine samples has been developed. Analysis of nucleosides using positive-ion mode with selected reaction monitoring effectively eliminated potential interferences from endogenous constituents of the urine. This highly selective and sensitive method made it possible to analyze urinary nucleosides with a lower limit of quantitation of 0.2 nmol/mL. The method has been validated, with both excellent linearity and reproducibility, in the calibration range from 0.2-400 nmol/mL. The correlation coefficients of the calibration curves were higher than 0.987. The coefficients of variation were in the range 0.03-14.92% (inter-day) and 0.54-14.39% (intra-day), respectively. Copyright # 2004 John Wiley & Sons, Ltd.Oxidized nucleosides represent excellent biomarkers for determining the extent of damage in genetic material, which has long been of interest in understanding the mechanism of aging, neurodegenerative diseases, and carcinogenesis. [1][2][3][4][5] Various methods have been developed for quantifying modified nucleosides, based on an enzyme-linked immunosorbent assay (ELISA), 6,7 32 P-post-labelling, 8 capillary electrophoresis, 9,10 HPLC with photodiode array detection 11,12 or ECD, 13 and GC/MS. 14-16 However, GC/MS analysis faced additional problems in the hydrolysis and derivatization steps. 17 HPLC/ECD analysis has limitations in that the requirement for electroactive components meant that the method was mainly applicable to the detection of only 8-oxodeoxyguanosine. 18 Due to their chemical characteristics in HPLC analysis, various kinds of nucleosides could not be analyzed using just one kind of detector.To overcome these problems, in the present work, we developed an analytical method for determining many kinds of nucleosides at one time using LC/MS with ESI (electrospray ionization) detection. We confirmed that mass spectrometric detection with ESI is more specific and sensitive than any other HPLC detection method. We also developed a direct analytical method for nucleosides without extracting urine samples. This approach is both more convenient and more efficient in conducting studies with large sample numbers, e.g., a clinical study, than analytical methods involving complex extrac...

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“…According to numerous reports, the detection limits (S/N = 3) of nucleosides could reach from 1.7 to 12.2 mM [41,48,56], and the resolution could be improved to 0.9 between 5-methyluridine and 3-methyluridine under optimum MEKC conditions [41]. The sensitivities for different cancer determinations were exciting in the reports: the diagnostic accuracy for thyroid cancer patients was at a ca.…”

Section: Modified Nucleosidesmentioning

confidence: 99%

Recent developments in the determination of urinary cancer biomarkers by capillary electrophoresis

Liu

et al. 2004

Electrophoresis

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Investigation of effective biomarkers for cancers is currently a popular area of study in clinical and cancer researches, because it can potentially lead to pre-cancer screening or pre-cancer diagnosis and may provide useful information on cancer type and the disease's stage of progression. More and more biochemical or chemical fluid components of the human body such as urine, blood, and cerebrospinal fluid have been considered to contain biomarkers, which are useful in cancer researches, pre-cancer diagnosis, and cancer follow-ups during or after cancer treatment. Several modern analytical techniques, such as gas chromatography (GC), high-performance liquid chromatography (HPLC), capillary electrophoresis (CE), and other separation techniques as well as hyphenated techniques, have been extensively used in study of cancer biomarkers. Among these techniques, CE is considered to be a highly efficient and practical analytical technique because of the small sample volume requirement and its wide separation versatility, ranging from small inorganic molecules to large biomolecules. This review discusses the latest developments involving biomarkers and their analysis by CE, including a discussion of instrumental conditions, method developments, and data analysis.

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Capillary electrophoretic profiling and pattern recognition analysis of urinary nucleosides from uterine myoma and cervical cancer patients

Cited by 46 publications

References 20 publications

Determination of urinary nucleosides by direct injection and coupled-column high-performance liquid chromatography

Determination of urinary nucleosides by direct injection and coupled-column high-performance liquid chromatography

A rapid and sensitive method for quantitation of nucleosides in human urine using liquid chromatography/mass spectrometry with direct urine injection

Recent developments in the determination of urinary cancer biomarkers by capillary electrophoresis

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