Capillary electrophoretic methods applied to the investigation of peptide complexes

Abstract

This article gives an overview of the applications of capillary electrophoretic methods to investigate the non-covalent interactions of peptides (peptide complexes) with variable middle- and high-molecular-mass receptors (ligands) as well as with small ions and molecules in the period 2007-2014. Different modes of capillary electrophoretic methods, such as mobility shift (vacancy) affinity capillary electrophoresis, multiple injection affinity capillary electrophoresis, partial filling affinity capillary electrophoresis, Hummel-Dryer method, vacancy peak method and (continuous) frontal analysis capillary electrophoresis, are briefly described and their applicability to determination of binding constants of peptide complexes is discussed. In addition, the detailed experimental conditions of individual applications and the values of binding constants of the particular peptide complexes are presented.

Investigation of multivalent interactions between conjugate of quantum dots with c‐Myc peptide tag and the anti‐c‐Myc antibody by capillary electrophoresis with fluorescence detection

“…CE–FL is widely used in biological, environmental, pharmaceutical, toxicology, clinical, and food analysis . A great application potential of CE for analysis of peptides and proteins and for investigation of their complexes has been found . The values were on the same order as those calculated from ELISA assays .…”

In‐depth insight into the methods of plasma protein‐drug interaction studies: Comparison of capillary electrophoresis‐frontal analysis, isothermal titration calorimetry, circular dichroism and equilibrium dialysis

“…Many CE‐based experimental approaches have been developed for the study of biomolecular interactions, utilizing the changes in the mobility of the interaction partners after their association or estimation of the amounts of free and bound partners . They are characterized by several important benefits such as no need for highly purified samples, immobilization, and labelling of any of the interacting partners.…”

Simultaneous monitoring of quantum dots and their assembly and disassembly with PreScission protease using capillary electrophoresis with fluorescence detection

“…In fact, there are several analytical methods for investigating the protein-peptide interaction or antibody-antigen interaction, such as SPR [15,16], ELISA [17,18], isothermal calorimetry (ITC) [19,20], high-performance size-exclusion chromatography (HPSEC) [21,22], microarray [23][24][25][26][27][28], CE [29][30][31], and other methods. Of them, SPR, ELISA, and microarray require immobilization of biomolecules.…”