Capillary electrophoretic method for the simultaneous determination of nitrate, nitrite and bromate ions in some selected plants

Abstract

BACKGROUND: Teas and traditional herbs are taken as health remedies in most cultures worldwide. Plants often used in the manufacture of teas and herbal remedies can comprise carcinogenic contaminates, such as nitrates, nitrites and bromate ions. In the present study, a quick method was applied for the simultaneous determination of nitrate (NO 3 − ), nitrite (NO 2 −) and bromate (BrO 3 − ) ions in some selected plants and tea samples using capillary electrophoresis and a Box-Behnken response surface design for method optimization.RESULTS: Optimal conditions of 60 mmol L -1 phosphate buffer solution at pH 3.5, a capillary temperature of 40°C, applied voltage of −22.5 kV, total capillary length of 61.5 cm (effective length 53.5 cm) with capillary internal diameter of 50 ∼m, and an injection pressure of 50 mbar for 30 s were selected. The limits of detection for nitrate, bromate and nitrite were 0.49, 1.25 and 0.11 mg L −1 , whereas linearity values were determined as 0.998, 0.996 and 0.999, respectively. CONCLUSION: A quick and easy applicable method was developed and the detection of the anions was achievable within 6 min of analysis. From the studied samples (cherry stalk, a dry mixed herbal tea; cherry stalk, dry sticks; dry pomegranate flower; dry olive leaves; and black tea), dry pomegranate flower (13.10 ± 0.62 mg L −1 ) and cherry stalk, dry mix herbal tea (13.10 ± 0.62 mg L −1 ) had the highest nitrate content.

Capillary electromigration methods for food analysis and Foodomics: Advances and applications in the period March 2021 to March 2023

“…Although pH 3.25 was the optimum value for the separation, another important parameter that was considered in this study was the total analysis time, and pH 3.5 reduced the migration time for both the nitrate and bromate anions. The content of nitrite, nitrate, and bromate ions in the samples was related to farming practices during the growing process of the plants, which means that the level of these hazardous analytes could be reduced with good harvest practices reducing the health risk of consumers [71]. The presence of heavy metals in tea was also an objective of analysis by CE.…”

Fabrication of a Disposable Amperometric Sensor for the Determination of Nitrite in Food

“…Therefore, monitoring whether the amount of nitrite in food is excessive is essential to protect human health, requiring the accurate and timely monitoring of nitrite in food samples. Some methods for the determination of nitrite have been proposed in recent years, such as spectrophotometry [ 4 ], the chemiluminescence method [ 5 ], capillary ion chromatography [ 6 ], capillary electrophoresis [ 7 ], the fluorescence method [ 8 ], the electrochemical sensing method [ 9 ], etc. Particularly, the electrochemical sensing method has attracted more attention because of its simple operation, high sensitivity, and rapid response time.…”

Development of a capillary electrophoresis method based on magnetic solid‐phase extraction for simultaneous and sensitive detection of eight biogenic amines in foods

“…11 Capillary electrophoresis (CE) has emerged as a good candidate in the applications of food science and environmental science due to many benefits, including simple sample preparation without derivatization and highly efficient separation. 12,13 Unfortunately, an important drawback for CE is low sensitivity due to the short optical path. 14 Consequently, searching for a pretreatment procedure capable to further improve sensitivity of CE is receiving widespread attention.…”

“…Nevertheless, generating highly specific and stable antibodies is still an urgent requirement due to the low molecular weight of BAs 11 . Capillary electrophoresis (CE) has emerged as a good candidate in the applications of food science and environmental science due to many benefits, including simple sample preparation without derivatization and highly efficient separation 12,13 . Unfortunately, an important drawback for CE is low sensitivity due to the short optical path 14 .…”