Capillary electrophoretic immunoassay for alpha-fetoprotein with chemiluminescence detection

Liu, Yanming; Mu, Hai-Bei; Zheng, Yan-Li; Wang, Chengquan; Chen, Yonghong; Li, Furong; Wang, Junhua; Cheng, Jie

doi:10.1016/j.jchromb.2007.04.033

Cited by 30 publications

(12 citation statements)

References 37 publications

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“…The basic design of the CE-CL system has been previously described [29]. Briefly, a high-voltage supply (0-30 kV, Shanghai Institute of Applied Physics, Chinese Academy of Sciences, China) was used to drive the electrophoresis.…”

Section: Instrumentationmentioning

confidence: 99%

Highly sensitive capillary electrophoretic immunoassay of rheumatoid factor in human serum with gold nanoparticles enhanced chemiluminescence detection

et al. 2014

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A new CE-based immunoassay method for the determination of rheumatoid factor was developed using chemiluminescent reaction of luminol and hydrogen peroxide catalyzed by gold nanoparticles (AuNPs). In this method, AuNPs were synthesized and conjugated with anti-RF (antibody, Ab) to form tagged Ab (AuNPs-Ab, Ab*), which subsequently linked to limited amount of RF (antigen, Ag) to produce Ab*-Ag complex by a noncompetitive immunoreaction. AuNPs were used to label antibody and amplify chemiluminescent signal. Under the optimized conditions, the mixture of free Ab* and Ab*-Ag complex was well separated and detected. This method yields a wide linear range of 0.01-20 μg/mL with a correlation coefficient of 0.997, and the detection limit of RF reaches 5.95 ng/mL (ca. 6.0 pmol/L, S/N = 3). The proposed method was successfully applied for the quantification of RF in human sera from patients with rheumatoid arthritis. This highly sensitive and selective method could be developed into a promising and useful technique for biological molecules determination in clinical analysis.

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Section: Instrumentationmentioning

confidence: 99%

Highly sensitive capillary electrophoretic immunoassay of rheumatoid factor in human serum with gold nanoparticles enhanced chemiluminescence detection

et al. 2014

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“…Hydrogen peroxide is still very used as oxidant in the luminol reaction, in spite of the previously mentioned disadvantages, in conjunction with a catalyst such as a metal ion [97][98][99] or horseradish peroxidase (HRP) [100][101][102][103][104][105][106][107][108]. The preferred metal ions to catalyze the luminol reaction are Co(II) and Cu(II) and both direct and indirect detections are possible.…”

Section: Clinical and Pharmaceutical Applicationsmentioning

confidence: 99%

“…This method allows the baseline separation of HRP complexes from free HRP in less than 3 min, and can be successfully applied to arteriosclerosis pathology research. Similar CEIA methodologies using HRP and p-iodophenol have been used for quantification of CA125 in human sera from health controls and patients associated with ovarian cancer [101], to determine tumor markers, such as a-fetoprotein [104] or carcinoembryonic antigen [108] in human sera samples, to quantify in human serum a luteinizing hormone which control the menstrual cycle [105], or for the determination of clenbuterol in human urine [102] or hepatitis B surface antigen and antibody in human serum [107]. The enhancement effect of p-iodophenylboronic acid can be affected by the presence of mono-and disaccharides in the running buffer and this fact has been used to the molecular recognition of these saccharides [103,112].…”

Section: Clinical and Pharmaceutical Applicationsmentioning

confidence: 99%

Advances and analytical applications in chemiluminescence coupled to capillary electrophoresis

2010

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Advances and analytical applications in chemiluminescence coupled to capillary electrophoresisThe present review presents the state of the art of the developments, key strategies and analytical applications of chemiluminescence detection coupled to CE (CE-CL). Different parts considering the most common CL systems have been included, such as the tris(2,2 0 -bipyridine)ruthenium(II) system, the luminol and derivatives reaction, the peroxyoxalate CL or direct oxidations. New advances in homemade configurations and applications in different fields such as clinical, pharmaceutical, environmental and food analysis have been included. The focus is on studies which appeared from 2000 to the end of 2009.

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“…Since there is no need for an external energy supply, CL can provide higher sensitivity and simplicity compared with other detections, and it is uniquely suited to on-line detection for CE. CE combined CL (CE-CL) has been successfully used as an attractive scheme for the analysis of metal ions [7][8], organic substances [9][10][11][12], amino acids [13][14], and antigen-antibody complexes [15][16][17][18]. Typically, the CL systems consist of CL reagent, oxidant and catalyst.…”

Section: Introductionmentioning

confidence: 99%

Electrogenerated ferrate(VI) for CE–chemiluminescence detection to determine benzenediol isomers

Zhang

et al. 2010

J of Separation Science

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Based on the quenched chemiluminescence intensity of the luminol reaction sensitized by ferrate(VI) in alkaline medium, a novel CE with on-line inhibited method for the simultaneous analysis of benzenediol isomers was developed. The ferrate(VI) solution was freshly prepared by electrochemical technique before electrophoresis. The parameters influencing detection and separation were carefully investigated. Baseline separation of benzenediols including catechol, resorcinol, and hydroquinone was achieved in less than 8 min with 5.0 mmol/L sodium tetraborate and 2.0 mmol/L luminol at an applied voltage of 18 kV. The LODs (S/N=3) for catechol, resorcinol and hydroquinone were determined to be 8.5 x 10(-9), 8.0 x 10(-7) and 6.5 x 10(-9) mol/L, respectively. Finally, the proposed method was applied for phenolic compounds in hair dye.

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Capillary electrophoretic immunoassay for alpha-fetoprotein with chemiluminescence detection

Cited by 30 publications

References 37 publications

Highly sensitive capillary electrophoretic immunoassay of rheumatoid factor in human serum with gold nanoparticles enhanced chemiluminescence detection

Highly sensitive capillary electrophoretic immunoassay of rheumatoid factor in human serum with gold nanoparticles enhanced chemiluminescence detection

Advances and analytical applications in chemiluminescence coupled to capillary electrophoresis

Electrogenerated ferrate(VI) for CE–chemiluminescence detection to determine benzenediol isomers

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