Capillary electrophoretic determination of selenocyanate and selenium and tellurium oxyanions in bacterial cultures

Abstract

Experiments were also designed to detect the biological production of selenocyanate in Se-amended bacterial cultures using IC-ICP-MS. A selenium-resistant bacterium, 130404, when amended with 1.0 mM selenate biologically produced selenocyanate in replicate cultures grown to stationary phase. The amounts produced were statistically significantly different than the amounts of SeCN -produced in sterile but warmed growth media. The headspace gases above growing cultures with or without metalloid amendments were analyzed using gas chromatography. Organo-selenium iii compounds that were detected were methaneselenol, dimethyl selenide, dimethyl selenenyl sulfide, dimethyl diselenenyl sulfide, dimethyl diselenide. Two late eluting peaks were observed in the same cultures that were not characterized but can reasonably be inferred to be dimethyl diselenenyl sulfide and dimethyl triselenide.

Element Speciation Analysis Using Capillary Electrophoresis: Twenty Years of Development and Applications

“…Typically, a quaternary ammonium compound, such as cetyltrimethylammonium chloride (CTAC), ,, hydroxide, ,,, or bromide, ,,,,, is introduced as an additive to the running electrolyte used. Others that have proven useful are tetradecyltrimethylammonium bromide ,,,,,,, or hydroxide, ,,,,,, dodecyltrimethylammonium bromide, , hexamethonium bromide , or hydroxide, , octadecyltrimethylammonium hydroxide, etc. , It should be noted that in general these EOF modifiers are not always inert with respect to the anionic analytes. ,, By interacting with them, they may induce not only alterations in the resolution (by an ion-exchange mechanism) but also compromise the separation efficiency, cause signal suppression, and even interfere with some detection modes. Therefore, amines can be used instead of the surfactants.…”

Detection of Selenocyanate in Biological Samples by HPLC with Fluorescence Detection Using König Reaction

“…Some relevant analytical methods have been reported. 1,[5][6][7] Anan et al used inductively coupled plasma mass spectrometry (ICP-MS) to detect SeCN − in mammalian cells but the method is time-and cost-intensive. On the other hand, Vasuki et al have reported that the König reaction, which is used for the determination of cyanide and thiocyanate, [8][9][10] is applicable to SeCN − detection.…”

Inorganic species analysis by CE – An overview for 2007–2008

“…Large‐volume sample stacking provided CE‐UV a similar detectability that allowed for quantification of phenylarsenic species in the case of aqueous extracts from heavily contaminated soils 51. A CE‐UV system enabling simultaneous separation of oxyanions of selenium and tellurium (and another environmentally important Se anion, selenocyanate) has been applied to monitor the bioconversion of metalloid‐containing anions in live cultures of two different bacteria 52.…”