Capillary electrophoresis with laser-induced native fluorescence detection for profiling body fluids

Paquette, Donald M.; Sing, Robert; Banks, Peter; Waldron, Karen C.

doi:10.1016/s0378-4347(98)00118-2

Cited by 49 publications

(24 citation statements)

References 33 publications

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“…In order to quantitatively determine the concentrations of the three identified analytes, we spiked the standards to one of the urine sample. Using the regression lines listed in Table 3, the concentrations of TA, 5-HT, and Trp were calculated, with the results of 0.50 6 0.02, 0.49 6 0.04, and 74 6 2, respectively, which are in good agreement with the reported data [48,49]. Other conditions are the same as in Fig.…”

Section: Urine Analysissupporting

confidence: 83%

Silica nanoparticles for separation of biologically active amines by capillary electrophoresis with laser‐induced native fluorescence detection

2005

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This paper describes the analysis of biologically active amines by capillary electrophoresis (CE) in conjunction with laser-induced native fluorescence detection. In order to simultaneously analyze amines and acids as well as to achieve high sensitivity, 10 mM formic acid solutions (pH < 4.0) containing silica nanoparticles (SiNPs) were chosen as the background electrolytes. With increasing SiNP concentration, the migration times for seven analytes decrease as a result of increase in electroosmotic flow (EOF) and decrease in their electrophoretic mobilities against EOF. A small EOF generated at pH 3.0 reveals adsorption of SiNPs on the deactivated capillary wall. The decreases in electrophoretic mobilities with increasing SiNP concentration up to 0.3x indicate the interactions between the analytes and the SiNPs. Having a great sensitivity (the limits of detection at a signal-to-noise ratio (S/N) = 3 of 0.09 nM for tryptamine (TA)), high efficiency, and excellent reproducibility (less than 2.4% of the migration times), this developed method has been applied to the analysis of urinal samples with the concentrations of 0.50 +/- 0.02 microM, 0.49 +/- 0.04 microM, and 74 +/- 2 microM for TA, 5-hydroxytryptamine, and tryptophan, respectively. The successful examples demonstrated in this study open up a possibility of using functional nanoparticles for the separation of different analytes by CE.

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Section: Urine Analysissupporting

confidence: 83%

Silica nanoparticles for separation of biologically active amines by capillary electrophoresis with laser‐induced native fluorescence detection

2005

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“…In order to quantitatively determine the concentrations of the identified analytes, we spiked the standards to one of the urine sample. The results are listed in Table 5, which are comparable and are both in a good agreement with the reported data [39,40]. The concentration of TA was not determined when using the (PLL-SiO 2 NP) 3 capillary, mainly because of the corresponding peak was not well resolved from another peak.…”

Section: Urine Analysissupporting

confidence: 89%

Capillary electrophoretic separation of biologically active amines and acids using nanoparticle‐coated capillaries

et al. 2008

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This manuscript describes dynamic coating of capillaries with poly(L-lysine) (PLL) and silica nanoparticles (SiO 2 NPs) and use of the as-prepared capillaries for the separation of biogenic amines and acids by CE in conjunction with LIF detection. The directions of EOF are controlled by varying the outmost layer of the capillaries with PLL and SiO 2 NPs, respectively. Over the pH range 3.0-5.0, the (PLL-SiO 2 NP) n -PLL capillaries have an EOF toward the anodic end and are more suitable for the separation of acids with respect to speed, while the (PLL-SiO 2 NP) n capillaries have an EOF toward the cathodic end and are more suitable for the separation of biogenic amines regarding speed and sensitivity. The separations of standard solutions containing five amines and two acids by CE with LIF detection using (PLL-SiO 2 NP) 2 -PLL and (PLL-SiO 2 NP) 3 capillaries were accomplished within 10 and 7 min, providing plate numbers of 3.8 and 5.0610 4 plates/m for 5-hydroxytryptamine (5-HT), respectively. The LODs for 5-HT and 5-hydroxyindole-3-acetic acid (5-HIAA) are 32 and 2 nM and 0.2 and 1.5 nM when using the (PLL-SiO 2 NP) 2 -PLL and (PLL-SiO 2 NP) 3 capillaries, respectively. Identification and quantification of 5-HIAA, homovanillic acid, and DL-vanillomandelic acid in urine samples from a male before and after drinking green tea were tested to validate practicality of the present approach. The results show that the (PLLSiO 2 NP) 2 -PLL capillary provides greater resolving power, while the (PLL-SiO 2 NP) 3 capillary provides better sensitivity, higher efficiency, and longer durability for the separation of the amines and acids.

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“…For example, the measurement of urinary 5-HIAA is important to diagnose carcinoid tumors. By applying a standard addition method, we estimated the concentrations of 5-HT and 5-HIAA, on the basis of the peak heights, are 0.15 nM (6.0% RSD) and 0.5 nM (5.4% RSD), respectively, which are in good agreement with the literature [35,44]. Table 2 provides great enhancement in the sensitivity for the amines but needs a longer separation time.…”

Section: Analysis Of Urinesupporting

confidence: 82%

Discontinuous electrolyte systems for improved detection of biologically active amines and acids by capillary electrophoresis with laser-induced native fluorescence detection

Hsieh

Chang

2005

Electrophoresis

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On-line concentration and separation of biologically active amines and acids by capillary electrophoresis (CE) in conjunction with laser-induced fluorescence using an Nd:YAG laser at 266 nm under discontinuous conditions is presented. The suitable conditions for simultaneous analysis of amines and acids were: samples were prepared in a solution (pH* 3.1) consisting of 10 mM citric acid, 89% acetonitrile (ACN), and water; a capillary was filled with 1.5 M Tris-borate (TB) buffer (pH 10.0); and the anodic vial contained PTG10 buffer (pH* 9.0) that consists of 50 mM propanoic acid, Tris, 10% glycerol, and water. After injecting a large-volume sample, amines and acids were separately stacked at the front (cathodic side) and back (anodic side) of the acidic sample zone, mainly because of changes in their electrophoretic mobilities as a result of changes in pH, viscosity, and electric field when high voltage was applied. When the sample was injected at 15 kV for 360 s, the concentration limits of detection (LODs) for 5-hydroxytryptamine (5-HT) and 5-hydroxyindole-3-acetic acid (5-HIAA) were 0.27 and 0.31 nM, respectively, which are about 400- and 800-fold sensitivity improvements when compared to those injected at 1 kV for 10 s. For the analysis of amines, samples were prepared in 100 mM citric acid (pH* 1.8) containing 89% ACN and both the capillary and anodic vial were filled with 400 mM PTG20 (propanoic acid, Tris, 20% glycerol, and water) at pH* 4.5. Using a large injection volume (15 kV for 360 s), we achieved concentration LODs of 17 pM and 0.3 nM for tryptamine and epinephrine, which are about 5200- and 14,000-fold sensitivity improvements, respectively, in comparison with those injected at 1 kV for 10 s. The features of simplicity (no sample pretreatment), rapidity (12 min), and sensitivity for identification of amines and acids of interest in urine samples show diagnostic potential of the two approaches developed in this study.

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Capillary electrophoresis with laser-induced native fluorescence detection for profiling body fluids

Cited by 49 publications

References 33 publications

Silica nanoparticles for separation of biologically active amines by capillary electrophoresis with laser‐induced native fluorescence detection

Silica nanoparticles for separation of biologically active amines by capillary electrophoresis with laser‐induced native fluorescence detection

Capillary electrophoretic separation of biologically active amines and acids using nanoparticle‐coated capillaries

Discontinuous electrolyte systems for improved detection of biologically active amines and acids by capillary electrophoresis with laser-induced native fluorescence detection

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