Capillary electrophoresis with electrospray mass spectrometry detection for low-molecular-mass compounds

Wheat, Thomas E.; Lilley, Kim A.; Banks, J. Fred

doi:10.1016/s0021-9673(97)00372-5

Cited by 42 publications

(30 citation statements)

References 13 publications

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“…Ion trap Sheath liquid a) FASS, field-amplified sample stacking in the presence of siphoning [326] Basic dendrimers CZE Magnetic sector Sheath liquid [327] Low-molecular-mass compounds CZE Single quadrupole Sheath liquid [328] Small carboxylic acids CZE Single quadrupole Sheath liquid [329] a-and b-chains of hemoglobin of a single intact red blood cell CZE TOF In-column electrode [330] Trace impurities of peptides and alkaloids CZE Triple quadrupole Make-up flow [331] Amino acids CZE Single quadrupole Sheath liquid…”

Section: ] Capillary Isotachophoresis Esi-ms (Citp-esi-ms) A)mentioning

confidence: 99%

Capillary electrophoresis – mass spectrometry: 15 years of developments and applications

Schmitt‐Kopplin¹,

Frommberger²

2003

Electrophoresis

281

232

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Capillary electrophoresis -mass spectrometry: 15 years of developments and applicationsSince its introduction in 1987, capillary electrophoresis-mass spectrometry (CE-MS) has developed to a well accepted multidimensional analytical approach complementary and/or competitive to classical MS-hyphenated separation techniques. The threefold combination of rapid developments of an exceptional separation technique, of selective mass detection possibilities, and of very mild ionization modes first allowed these progresses. This article shows the CE specificities that need to be well controlled/known, compared to classical and more routinely used liquid chromatography in the light of its coupling to MS. The major trends and developments over the last 15 years and most of the reviews and applications found in ISI Web of science and publisher databases are presented in a tabulated way. The reader can thus rapidly find existing CE-MS analysis techniques in his field of research and application (forensics, environment, bioanalytics, pharmaceutics, and metabolites).

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Section: ] Capillary Isotachophoresis Esi-ms (Citp-esi-ms) A)mentioning

confidence: 99%

Capillary electrophoresis – mass spectrometry: 15 years of developments and applications

Schmitt‐Kopplin¹,

Frommberger²

2003

Electrophoresis

281

232

View full text Add to dashboard Cite

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“…On the contrary, a sensitivity decrease (factor about 2) was observed in the presence of formic acid. In contrast to ESI, where an acid in the sheath liquid is mandatory to assist positive ionization [56,[59][60][61], the presence of a protic solvent in the sheath liquid appeared sufficient for photoionization.…”

Section: Sheath Liquid Compositionmentioning

confidence: 99%

Coupling CE with atmospheric pressure photoionization MS for pharmaceutical basic compounds: Optimization of operating parameters

Schappler¹,

Guillarme²,

Prat³

et al. 2007

Electrophoresis

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The use of CE coupled with MS (CE-MS) has evolved as a useful tool to analyze charged species in small sample volumes. Because of its sensitivity, versatility and ease of implementation, the ESI interface is currently the method of choice to hyphenate CE to MS. An alternative can be the atmospheric pressure photoionization (APPI) source, however, numerous parameters must be optimized for its coupling to CE. After evaluation of the sheath liquid composition and the CE capillary outlet position, an experimental design methodology was assessed for optimizing other ionization source parameters, such as sheath liquid flow rate, drying gas flow rate and temperature, nebulizing gas pressure, vaporizer temperature, and capillary voltage. For this purpose, a fractional factorial design (FFD) was selected as a screening procedure to identify factors which significantly influence sensitivity and efficiency. A face-centered central composite design (CCD) was then used to predict and optimize sensitivity, taking into account the most relevant variables. Sensitivity was finally evaluated with the optimized conditions and height-to-noise ratios (H/N) around 10 were achieved for an injection of 200 ng/mL of each analyte.

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“…This is related to the ability of CE to separate complex mixtures of analytes while sample consumption is only minor [20]. In addition, high separation efficiency with up to 10 6 theoretical plates allows for rapid separations within a couple of minutes in most cases [21]. High efficiency and mass sensitivity is promoted by the absence of Eddy-diffusion and mass transfer, both factors that contribute to peak broadening in HPLC [19,22].…”

mentioning

confidence: 99%

Advances in the analysis of proteins and peptides by capillary electrophoresis with matrix‐assisted laser desorption/ionization and electrospray‐mass spectrometry detection

2005

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High throughput, outstanding certainty in peptide/protein identification, exceptional resolution, and quantitative information are essential pillars in proteome research. Capillary electrophoresis (CE) coupled to mass spectrometry (MS) has proven to meet these requirements. Soft ionization techniques, such as matrix-assisted laser desorption/ionization (MALDI) and electrospray ionization (ESI), have paved the way for the story of success of CE-MS in the analysis of biomolecules and both approaches are subject of discussion in this article. Meanwhile, CE-MS is far away from representing a homogeneous field. Therefore the review will cover a vast area including the coupling of different modes of CE (capillary zone electrophoresis, capillary isoelectric foscusing, capillary electrochromatography, micellar electrokinetic chromatography, nonaqueous capillary electrophoresis) to MS as well as on-line preconcentration techniques (transient capillary isotachophoresis, solid-phase extraction, membrane preconcentration) applied to compensate for restricted detection sensitivity. Special attention is given to improvements in interfacing, namely addressing nanospray and coaxial sheath liquid design. Peptide mapping, collision-induced dissociation with subsequent tandem MS, and amendments in mass accuracy of instruments improve information validity gained from MS data. With 2-D on-line coupling of liquid chromatography (LC) and CE a further topic will be discussed. A special section is dedicated to recent attempts in establishing CE-ESI-MS in proteomics, in the clinical and diagnostic field, and in the food sector.

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Capillary electrophoresis with electrospray mass spectrometry detection for low-molecular-mass compounds

Cited by 42 publications

References 13 publications

Capillary electrophoresis – mass spectrometry: 15 years of developments and applications

Capillary electrophoresis – mass spectrometry: 15 years of developments and applications

Coupling CE with atmospheric pressure photoionization MS for pharmaceutical basic compounds: Optimization of operating parameters

Advances in the analysis of proteins and peptides by capillary electrophoresis with matrix‐assisted laser desorption/ionization and electrospray‐mass spectrometry detection

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