Capillary electrophoresis: the state-of-the-art in metal speciation studies

Da̧bek-Złotorzyńska, Ewa; Lai, Edward P. C.; Timerbaev, Andrei R.

doi:10.1016/s0003-2670(97)00635-1

Cited by 116 publications

(71 citation statements)

References 86 publications

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“…In recent years, there has been an increasing interest in application of CE for separation and determination of ionic species [24][25][26]. Many speciation analyses were carried out by CE inductively coupled plasma atomic emission spectrometry (ICP-AES) and other hyphenated techniques [27][28][29][30][31][32].…”

Section: Introductionmentioning

confidence: 99%

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Speciation of magnesium in rat plasma using capillary electrophoresis‐inductively coupled plasma‐atomic emission spectrometry

Deng

Zhu

et al. 2008

Electrophoresis

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A new method for speciation analysis of magnesium species and quantification of free magnesium concentrations in rat plasma was developed by on-line coupling of CE with inductively coupled plasma-atomic emission spectrometry (ICP-AES). Baseline separation of seven magnesium species was achieved by using a 120 cm (100 mm internal diameter) fused-silica capillary, a 20 kV separation voltage and a solution of 50 mmol/L NaAc-HAc (pH 5.5) as electrolyte buffer. CE-ICP-AES analysis of a rat plasma sample showed the presence of seven magnesium species, one of which was identified as free Mg 21 ion by spiking a Mg 21 standard; the migration time of the Mg 21 peak in the standard and the spiked sample matched with each other. One protein-bound magnesium species in rat plasma is associated with albumin, and the other three species are combined with globulin. The concentration of free magnesium in the plasma was 14.0 mg/L. The other six magnesium species were estimated to be 4-15 mg/L. RSDs of migration time and peak area for the magnesium species from ten replicates were less than 5%. The developed method was also applied to speciation analysis of magnesium species in spiked plasma samples. The recoveries of the free magnesium species in four samples ranged from 95.8 to 103.8%.

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Section: Introductionmentioning

confidence: 99%

“…CE is a powerful yet relatively simple separation technique for a wide range of samples in environmental, biological and clinical analysis [42][43][44]. Coupling CE to ICP spectrometry, which is a powerful method for metal speciation, further broadens the range of CE applications [45][46][47][48][49][50][51][52].…”

Section: Introductionmentioning

confidence: 99%

Speciation of magnesium in rat plasma using capillary electrophoresis‐inductively coupled plasma‐atomic emission spectrometry

Deng

Zhu

et al. 2008

Electrophoresis

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“…These methods have shown high selectivity, excellent linearity and reproducibility in analysis for MeHg and other forms of Hg. 31 A disadvantage of using ICP as a detector for HPLC is that the mobile phase should be carefully chosen as solvents such as methanol and acetonitrile are not compatible with the argon plasma. 2 Mobile phases which contain high concentrations of salts and buffers can clog the cones of the nebulizer which can produce changes in sensitivity.…”

Section: Introductionmentioning

confidence: 99%

Determination of mercury in selected polluted sediments using HPLC-ICP-MS in Westbank area, Western Cape, South Africa

Roux¹,

Baker²,

Crouch³

2016

S.Afr.j.chem.

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High selectivity, excellent linearity and reproducibility were achieved using HPLC-ICP-MS method for mercury in sediments. The HPLC-ICP-MS method reported previously for mercury in biota analysis was adapted in terms of operational consumables and optimized for the efficient mercury determination in riverbed sediments; a first for South Africa. Results obtained for real environmental samples, were verified by Direct Mercury Analysis (DMA) by an accredited laboratory. The determination of inorganic and organic mercury compounds in sediments was based on the complexation of mercury compounds with cysteine. The separation was achieved on a reversed-phase C 18 column (15 cm × 4.6 mm id) using aqueous 0.1 % w/v L-cysteine·HCl + 0.1 % w/v L-cysteine mobile phase at a flow rate of 0.8 mL min -1 at ambient temperature. The method was found to be suitable for the routine analysis of Hg compounds in sediments as well as aqueous media and was developed to determine the influence of humic substances on the bioavailability of mercury species. The results were comparable and consistent with those determined with a Direct Mercury Analyzer. The detection limit for methylmercury, ethylmercury and inorganic mercury were lower than 10 ng L -1 and recoveries for spiked samples was >90 % . The accuracy of the method was assessed by the analysis of certified reference material (European Reference Material ERMCC580 and Tort-2) and found to be in good agreement. Mercury concentration was determined in sediments collected from the Eerste Kuils River, Westbank area, Kuilsriver, Western Cape, South Africa. Mercury concentration was determined to vary from 2-14 ng g -1 (w.wt).

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“…Fritz et al [5] reported using a complexing electrolyte to separate 27 cations. Thus, CE has made great advances [11][12][13][14]. However, the determination of inorganic ions in beverages is still a challenge due to the large variability in both their identities and concentrations.…”

Section: Introductionmentioning

confidence: 99%

Separation and determination of inorganic cations in beverages by capillary electrophoresis with indirect UV detection

et al. 2008

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Capillary electrophoresis: the state-of-the-art in metal speciation studies

Cited by 116 publications

References 86 publications

Speciation of magnesium in rat plasma using capillary electrophoresis‐inductively coupled plasma‐atomic emission spectrometry

Speciation of magnesium in rat plasma using capillary electrophoresis‐inductively coupled plasma‐atomic emission spectrometry

Determination of mercury in selected polluted sediments using HPLC-ICP-MS in Westbank area, Western Cape, South Africa

Separation and determination of inorganic cations in beverages by capillary electrophoresis with indirect UV detection

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