Capillary electrophoresis of heparin and other glycosaminoglycans using a polyamine running electrolyte

Loegel, Thomas N.; Trombley, John D.; Taylor, Richard T.; Danielson, Neil D.

doi:10.1016/j.aca.2012.09.019

Cited by 17 publications

(15 citation statements)

References 17 publications

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“…and . The results suggest that the migration time increase with increase of concentration of EDA, which may be due to the ion‐pair formation between EDA and DS, CS, lowering the effective negative charge, and slowing the GAG movement through the capillary . EOF decreased in the concentration range of 50–200 mM, which may be due to the coating of EDA on the surface of the capillary and effectively creating charge on the surface of the silica .…”

Section: Resultsmentioning

confidence: 93%

“…At present, the buffer systems for analysis of GAGs have phosphate, borate, Tris, and polyamine; among them the polyamine as running electrolyte can obtain better result. Reference showed the optimum separation conditions of CS and DS is 50 mM EDA, 18.7 mM phosphate, pH 4.5, capillary temperature of 34.5°C, and a voltage of −30 kV, using a 50 μm × 51.5 cm effective capillary length. Based on the reference, the separation conditions were further optimized.…”

Section: Resultsmentioning

confidence: 99%

“…(iv) The interaction between EDA and analytes and the internal of capillary. The conductivity test in reference showed that EDA and CS and DS could form the ion pair and slow the GAG movement through the capillary, EDA with positive charge could coat on the surface of the capillary and decrease the EOF. In summary, the improvement in separation efficiency was a collective result of a modified EOF, an ion‐exchange mechanism , and a reduction in the molecular movement.…”

Section: Resultsmentioning

confidence: 99%

“…Recently, Danielson et al. developed a CE method using polyamine as running electrolyte for the analysis of intact DS and CS, but peak shape and resolution of DS and CS are not satisfactory.…”

Section: Introductionmentioning

confidence: 99%

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Gold nanomaterials based pseudostationary phases in capillary electrophoresis: A brand‐new attempt at chondroitin sulfate isomers separation

Zhao

Zhou

et al. 2015

Electrophoresis

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In this work, a CE method with bare gold nanorods (GNRs) based pseudostationary phase was developed and applied for the separation of chondroitin sulfate (CS) isomers, CS, and dermatan sulfate (DS). The separation efficiency was investigated by varying the experimental parameters such as concentration and pH of the BGE, separation voltage, internal diameter of capillary, different size, and morphology of gold nanomaterials. Results showed that different size and morphology of gold nanomaterials had different effects on the separation of CS and DS. The best separation of CS and DS was achieved in the BGE composed of aqueous 150 mmol/L (mM) ethylenediamine + 20 mM sodium dihydrogen phosphate + 30% v/v GNRs, pH 4.5, at the separation voltage of -10 kV. Capillary was 59.2 cm in length (effective length 49 cm), 50 μm id capillary thermostated at 25°C. CE with bare GNRs used as pseudostationary phase was shown to be a suitable technique for the separation of CS and DS mixtures with wider peaks. RSD of migration time and peak area of CS and DS were 0.13, 0.14 and 0.86, 1.07%, respectively.

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Section: Resultsmentioning

confidence: 93%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Gold nanomaterials based pseudostationary phases in capillary electrophoresis: A brand‐new attempt at chondroitin sulfate isomers separation

Zhao

Zhou

et al. 2015

Electrophoresis

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“…Because of the possibility of injecting relatively large sample volumes and high concentrations of heparin, OSCS could be detected down to the 0.1% level, despite the weak UV absorbance of these sulfated glycosaminoglycans. Loegel et al [57] developed a CE method for the separation of heparin, dermatan sulfate, chondroitin sulfate (or hyaluronan), and the impurity of OSCS using polyamine-containing electrolyte, ethylenediamine (EDA). It was possibly one of the first CE methods which successfully made use of a 50 m ID fused silica capillary for analysis of OSCS impurity in heparin samples.…”

Section: Capillary Electrophoresismentioning

confidence: 99%

Determination of Strong Acidic Drugs in Biological Matrices: A Review of Separation Methods

Xie

et al. 2014

Chromatography Research International

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Strong acidic drugs are a class of chemical compounds that normally have high hydrophilicity and large negative charges, such as organophosphatic compounds and organosulphonic compounds. This review focuses on sample preparation and separation methods for this group of compounds in biological matrices in recent years. A wide range of separation techniques, especially chromatographic method, are presented and critically discussed, which include liquid chromatography (e.g., ion-pair and ion-exchange chromatography), capillary electrophoresis (CE), and other types. Due to the extremely low concentration level of target analytes as well as the complexity of biological matrices, sample pretreatment methods, such as dilute and shoot methods, protein precipitation (PP), liquid-liquid extraction (LLE), solid-phase extraction (SPE), degradation, and derivatization strategy, also play important roles for the development of successful analytical methods and thus are also discussed.

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Proteoglycans and Acidic Polysaccharides Analysis

Lauder

2015

Encyclopedia of Analytical Chemistry

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The study of the structure and function of proteoglycans (PGs) and glycosaminoglycans (GAGs) has assumed an increasing significance as the wide range of important biological processes in which they play central roles has been elucidated. As studies of GAG structure expand in scope, and detail, the full structural diversity of these polymers is being uncovered. Significant heterogeneity can be seen but relationships with development, age, and pathology are being uncovered, which place these macromolecules centrally in these processes. Available methodologies allow detailed analysis of GAGs, but careful attention to the sample requirements of these methods and to their limitations is required to ensure the quality and reliability of the data derived is assured. Important developments, including mass spectrometry approaches, will take the field forward but well‐established methods for the isolation and characterization of these complex molecules continue to have a hugely important role to play in structural and functional studies. This article examines a wide range of methodologies for the isolation and characterization of the PGs and GAGs in which recent developments in the field are reported along with well‐established methods for the characterization of entire GAG chains or discrete features within those.

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Capillary electrophoresis of heparin and other glycosaminoglycans using a polyamine running electrolyte

Cited by 17 publications

References 17 publications

Gold nanomaterials based pseudostationary phases in capillary electrophoresis: A brand‐new attempt at chondroitin sulfate isomers separation

Gold nanomaterials based pseudostationary phases in capillary electrophoresis: A brand‐new attempt at chondroitin sulfate isomers separation

Determination of Strong Acidic Drugs in Biological Matrices: A Review of Separation Methods

Proteoglycans and Acidic Polysaccharides Analysis

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