Capillary electrophoresis of catecholamines with laser‐induced fluorescence intensified charge‐coupled device detection

Xiong, Shaoxiang; Han, Huiwan; Zhao, Rui; Chen, Yi; Liu, Guoquan

doi:10.1002/bmc.37

Cited by 36 publications

(25 citation statements)

References 31 publications

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“…Several detection techniques in CE, including LIF [20][21][22], UV [23,24], MS [25], EC [26][27][28], and fluorescence [29], have been developed to determine catecholamines. Although LIF detection has the advantage of high sensitivity, the disadvantages are expensive instrumentation and complicated approach.…”

Section: Introductionmentioning

confidence: 99%

Determination of catecholamines by CE with direct chemiluminescence detection

Liu

Wang

et al. 2007

Electrophoresis

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A rapid and sensitive method to detect three catecholamines, isoprenaline, epinephrine, and dopamine, by CE coupled with direct luminol-potassium periodate chemiluminescence (CL) detection is described. The conditions for CE separation and CL reaction were systematically optimized. Under the optimum conditions, the baseline separation of three catecholamines was achieved within 6.5 min. The LODs obtained in standard solution were 5.3 6 10 -8 mol/L for isoprenaline, 4.7 6 10 -8 mol/L for epinephrine, and 1.5 6 10 -7 mol/L for dopamine. The RSD of the migration time and peak area were less than 1.8 and 3.6% (n = 5), respectively. The present method was applied to the determination of the dopamine in urine samples of cigarette smokers and nonsmokers. The results obtained indicate that there is a close relationship between the content of dopamine in human urine and the amount of cigarettes smoked daily; the level of dopamine in smokers is higher than in nonsmokers.

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Section: Introductionmentioning

confidence: 99%

Determination of catecholamines by CE with direct chemiluminescence detection

Liu

Wang

et al. 2007

Electrophoresis

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“…According to the previous reports [18,21], excellent FITC derivatization could be achieved under weak alkaline conditions. This is because that the amino groups of the catecholamines would easily be deprotonated in alkaline buffer and the FITC fluorescent intensity in alkaline buffer rapidly increased.…”

Section: Effect Of the Ph Of Buffer On Derivatization And Separationmentioning

confidence: 74%

“…Derivatization of catecholamines with FITC was realized according to the procedures described previously [18]. Individual FITC-labeled E and DA solution was prepared by mixing 0.1 mL stock solution of E or DA with 0.5 mL of FITC reagent and diluting to 1.0 mL with 10 mM Na 2 B 4 O 7 /HCl (pH 9.2) buffer, and allowing to stand overnight in the dark at room temperature.…”

Section: Derivatization Proceduresmentioning

confidence: 99%

Separation of catecholamines by microchip electrophoresis with a simple integrated laser-induced fluorescence detector

Li¹,

Cai²,

Lin³

2006

Analytica Chimica Acta

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A simple microchip electrophoresis-laser-induced fluorescence device was constructed and used for separation and determination of catecholamines. On the fabricated glass chip, an extra optical fiber insertion channel, which was perpendicular and extremely close to the separation channel, was directly integrated by nothing operations more than design features on the photomask. The utilization of optical fiber to transmit the excitation light and the integration fiber channel make the fluorescence detection system simple and disposable. For electrophoresis, optimization of separation conditions was investigated for reaching high separation efficiency and sensitivity. A separation efficiency as high as 10 6 theoretical plate numbers could be obtained for the analytes.

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“…The coated PDMS devices were applied to analyze the biochemical sample of epinephrine with LIF detection. Epinephrine was labelled with FITC at a dye-to-epinephrine ratio of 5:2 [30]. A solution of 10 mM Na 2 B 4 O 7 /HCl (pH 9.0) was served as running buffer for the capillary zone electrophoresis separation.…”

Section: Dynamic Coating Of Fluidic Channels and Electrophoresismentioning

confidence: 99%

A polymeric master replication technology for mass fabrication of poly(dimethylsiloxane) microfluidic devices

Lin

et al. 2005

Electrophoresis

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A polymeric master replication technology for mass fabrication of poly(dimethylsiloxane) microfluidic devicesA protocol of producing multiple polymeric masters from an original glass master mold has been developed, which enables the production of multiple poly(dimethylsiloxane) (PDMS)-based microfluidic devices in a low-cost and efficient manner. Standard wetetching techniques were used to fabricate an original glass master with negative features, from which more than 50 polymethylmethacrylate (PMMA) positive replica masters were rapidly created using the thermal printing technique. The time to replicate each PMMA master was as short as 20 min. The PMMA replica masters have excellent structural features and could be used to cast PDMS devices for many times. An integration geometry designed for laser-induced fluorescence (LIF) detection, which contains normal deep microfluidic channels and a much deeper optical fiber channel, was successfully transferred into PDMS devices. The positive relief on seven PMMA replica masters is replicated with regard to the negative original glass master, with a depth average variation of 0.89% for 26 mm deep microfluidic channels and 1.16% for the 90 mm deep fiber channel. The imprinted positive relief in PMMA from master-to-master is reproducible with relative standard deviations (RSDs) of 1.06% for the maximum width and 0.46% for depth in terms of the separation channel. The PDMS devices fabricated from the PMMA replica masters were characterized and applied to the separation of a fluorescein isothiocyanate (FITC)-labeled epinephrine sample.

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Capillary electrophoresis of catecholamines with laser‐induced fluorescence intensified charge‐coupled device detection

Cited by 36 publications

References 31 publications

Determination of catecholamines by CE with direct chemiluminescence detection

Determination of catecholamines by CE with direct chemiluminescence detection

Separation of catecholamines by microchip electrophoresis with a simple integrated laser-induced fluorescence detector

A polymeric master replication technology for mass fabrication of poly(dimethylsiloxane) microfluidic devices

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