Capillary electrophoresis method for the analysis of inorganic anions, organic acids, amino acids, nucleotides, carbohydrates and other anionic compounds

Soga, Tomoyoshi; Imaizumi, Motohiro

doi:10.1002/1522-2683(200109)22:16<3418::aid-elps3418>3.0.co;2-8

Cited by 145 publications

(75 citation statements)

References 37 publications

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“…The solute composition in apoplast and tissue sap (2-4 lL of sap solution) was identified and quantified using an Agilent capillary electrophoresis (CE) system (G1600AX, Agilent Technologies, Germany). The CE analysis was performed according to Soga and Imaizumi (2001). Separations were carried out on fused silica capillaries with 50 lm i.d.…”

Section: Solute Potential Measurementsmentioning

confidence: 99%

Fruit ripening in Vitis vinifera: apoplastic solute accumulation accounts for pre-veraison turgor loss in berries

Wada

Shackel

Matthews

2008

Planta

102

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In Vitis vinifera L. berries, the onset of ripening (known as "veraison") involves loss of turgor (P) in the mesocarp cells. We hypothesized that P loss was associated with an accumulation of apoplastic solutes in mesocarp tissue prior to veraison. Apoplastic sap was extracted from the mesocarp by centrifugation at the appropriate gravity to measure the apoplast solute potential (Psi (s) (A) ) and assay the sap composition. The Psi (s) (A) was about -0.2 MPa early in development, decreased about 1.0 MPa by veraison, and continued to decrease during ripening to almost -4.0 MPa by the end of berry development. Potassium, malate, tartrate, proline, glucose, fructose, and sucrose were quantified in apoplastic sap. The calculated contribution of these solutes was about 50% of the total Psi (s) (A) preveraison, but increased to about 75% as fructose and glucose accumulated during ripening. The contribution of the estimated matric potential to apoplast water potential decreased during development and was only 1.5% postveraison. We conclude that high concentrations of solutes accumulated in the mesocarp apoplast prior to veraison, and that P loss was a direct result of decreased Psi (s) (A) . Because Psi (s) (A) decreased before veraison, our findings suggest that apoplast solutes play an important role in the events of cellular metabolism that lead to the onset of ripening.

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Section: Solute Potential Measurementsmentioning

confidence: 99%

Fruit ripening in Vitis vinifera: apoplastic solute accumulation accounts for pre-veraison turgor loss in berries

Wada

Shackel

Matthews

2008

Planta

102

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“…However, each technique has both advantages and limitations 21 and hence, a combination of different analytical techniques is commonly used. For example, GC-MS 22 , LC-MS [23][24][25] , CE-MS [26][27][28] have been used for analyzing a majority of metabolites.…”

Section: Methodsmentioning

confidence: 99%

Metabolomics - the new "omics" of health care

Vs¹,

PVLN²,

Bitla³

2012

JCSR

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Metabolic diseases are caused by chronic dysregulations of metabolism that differ among individuals due to interactions of the genetic and environmental influences manifesting as a particular phenotype. These are usually diagnosed with the help of individual metabolites which serve as disease biomarkers. However, single biomarker based diagnostics for metabolism-based diseases fails to identify the cause of the alteration of these surrogate markers. This is where metabolomics comes into the clinical picture as it not only helps in assessing the risk of an individual for developing a particular disease but also helps in individualizing therapy based on the metabolic defect. Metabolomics is named in line with other recently developed approaches of diagnostics, i.e., genomics and proteomics. This review provides an overview of the fundamental basic principles, the techniques involved and the applications of this new -omics of healthcare. Keywords: Metabolic disorders, Metabolomics, Health careKiranmayi VS, Srinivasa Rao PVLN, Bitla AR. Metabolomics -the new "omics" of health care. J Clin Sci Res 2012;3:131-7.

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“…The group of applications has also widened markedly. The applications of CE are covering huge number of analytes from inorganic ions [4][5][6][7][8] and organic molecules [9][10][11] to biomolecules such as proteins [12][13][14] and DNA [15][16][17]. The golden era of CE was in 1990s, during the Human Genome Project [18].…”

Section: From Nano-pottery To Modern Analytical Toolsmentioning

confidence: 99%

Capillary Electrophoresis in Nanotechnologies versus Nanotechnologies in Capillary Electrophoresis

Adam¹,

Vaculovičová²

2018

Novel Nanomaterials - Synthesis and Applications

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Nanomaterials are attracting an interest of many researches. All this attention is due the unique physical and chemical properties of nanomaterials differing significantly from the bulk materials mainly due to their size in range of nanometers. Capillary electrophoresis (CE) is a powerful, well-established analytical technique that provides numerous valuable benefits over other separation methods including high-performance liquid chromatography. The connection between CE and nanotechnology can be approached by two strategies: (i) CE analysis of nanomaterials and (ii) nanomaterials for CE improvement. The first perspective focuses on uses of CE as a method for characterization employed during nanomaterial production and modification as well as for monitoring their properties and interactions with other molecules. The second viewpoint deals with applications of nanomaterials for improving CE performance, mainly by enhancing efficiency of separation using nanomaterials as a stationary or pseudo-stationary phase and by enhancing detection sensitivity and/or selectivity in both optical and electrochemical detection. Moreover, applications of nanomaterials for sample preparation before CE analysis will be mentioned. This chapter aims at highlighting the symbiosis of CE and nanotechnology as a combination of modern, progressive field with well-known and reliable analytical method.

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Capillary electrophoresis method for the analysis of inorganic anions, organic acids, amino acids, nucleotides, carbohydrates and other anionic compounds

Cited by 145 publications

References 37 publications

Fruit ripening in Vitis vinifera: apoplastic solute accumulation accounts for pre-veraison turgor loss in berries

Fruit ripening in Vitis vinifera: apoplastic solute accumulation accounts for pre-veraison turgor loss in berries

Metabolomics - the new "omics" of health care

Capillary Electrophoresis in Nanotechnologies versus Nanotechnologies in Capillary Electrophoresis

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