Capillary Electrophoresis/Mass Spectrometry

Identification and quantitation of cis-ketoconazole impurity by capillary zone electrophoresis–mass spectrometry

“…Therefore, CE-ESI-MS was employed for the identification of this impurity in cis-ketoconazole because the possible impurities reported in the European Pharmacopoeia [16] have different masses (trans-ketoconazole of exact mass = 530.1487 g/mol; impurity II of exact mass = 528.1331 g/mol; impurity III of exact mass = 720.2481 g/mol and impurity IV of exact mass = 488.1382 g/mol). In order to obtain a limit of detection (LOD) as low as possible, the volatile BGE was used under achiral conditions because the presence of non-volatile components (e.g., cyclodextrins) in the BGE decreases the sensitivity, increases the background noise and can clog the system [18][19][20]. In addition, under achiral conditions, only one peak was observed for the impurity, providing a better sensitivity than that obtained for the two peaks observed under chiral conditions.…”

Application of capillary electrophoresis-electrospray ionization mass spectometry in the determination of molecular diversity.

“…When In this paper we describe the use of capillary electrophoresis (CE) coupled online with ESI-MS (7,8) to probe the synthesized combinatorial libraries for their diversity. CE generates rapid, high-resolution separations based on differences in the electrophoretic mobilities of charged species in an electric field in small-diameter fused-silica capillaries.…”

Annual Report 2000 Macromolecular Structure and Dynamics

“…It is important therefore to understand the real differences and similarities between DNA damage induced by normal oxidative processes versus low doses of ionizing radiation (Henle et al 1996, Dizdaroglu 1992. Recent dramatic advances in analytical methods (Smith et al 1993) 4-9 are now providing significantly more sensitive capabilities for efficiently and quantitatively detecting and characterizing the distinctive and chemically complex damage products from ionizing radiation (Hofstadler et al 1995).…”